Reviewed, UniProtKB/Swiss-Prot Q59634 (HYSA_PROAC)

Names and origin

Protein names

Recommended name:
    Hyaluronate lyase
    EC=4.2.2.1
Alternative name(s):
    Hyaluronidase
      Short name=HYase

Gene names

Ordered Locus Names:

PPA0380

Organism

Propionibacterium acnes [Complete proteome] [HAMAP]

Taxonomic identifier

1747 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Propionibacteriaceae › Propionibacterium

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine.
Subunit structure	Monomer.
Subcellular location	Secreted › cell wall.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the polysaccharide lyase 8 family.
Sequence caution	The sequence AAA51650.1 differs from that shown. Reason: Frameshift at positions 649 and 779.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hyaluronate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal
	Chain	33 – 813	781	Hyaluronate lyase		PRO_0000024930
Experimental info
	Sequence conflict	204	1	A → G in AAA51650. Ref.1
	Sequence conflict	304	1	V → D in AAA51650. Ref.1
	Sequence conflict	405 – 425	21	RPVPE…RLVHR → PRPGVVDAELLRVHGPSRPA in AAA51650. Ref.1
	Sequence conflict	454	1	S → Y in AAA51650. Ref.1
	Sequence conflict	649 – 657	9	VTHHDRPVA → GHPPRSSSP in AAA51650. Ref.1
	Sequence conflict	688 – 694	7	ATVQAVR → GNRPGRA in AAA51650. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59634-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 0C4316E7890D4D1D	FASTA	813	88,130
	10 20 30 40 50 60 MFGTPSRRTF LTASALSAMA LAASPTVTDA IAAPGPDSWS ALCERWIDII TGRRAARTSD 70 80 90 100 110 120 PRARAIIAKT DRKVAEILTD LVSGSSRQTV LISADLRKEQ SPFITKTARA IESMACAWAT 130 140 150 160 170 180 PGSSYHKDPE ILSACIEGLR DFCRLRYNPS QDEYGNWWDW EDGASRAVAD VMCILHDVLP 190 200 210 220 230 240 PEVMSAAAAG IDHFIPDPWF QQPASVKPTA NPVQPVVSTG ANRMDLTRAV MCRSIATGDE 250 260 270 280 290 300 KRLRHAVDGL PDAWRVTTEG DGFRADGGFI QHSHIPYTGG YGDVLFSGLA MLFPLVSGMR 310 320 330 340 350 360 FDIVESARKA FHDQVERGFI PVMYNGQILD DVRGRSISRI NESAAMHGIS IARAMLMMAD 370 380 390 400 410 420 ALPTHRAEQW RGIVHGWMAR NTFDHLSEPS TLVDISLFDA AAKARPVPES STPSYFASMD 430 440 450 460 470 480 RLVHRTADWL ITVSNCSDRI AWYEYGNGEN EWASRTSQGM RYLLLPGDMG QYEDGYWATV 490 500 510 520 530 540 DYSAPTGTTV DSTPLKRAVG ASWAAKTPTN EWSGGLASGS WSAAASHITS QDSALKARRL 550 560 570 580 590 600 WVGLKDAMVE LTTDVTTDAS RAITVVEHRK VASSSTKLLV DGNRVSSATS FQNPRWAHLD 610 620 630 640 650 660 GVGGYVFATD TDLSADVATR KGTWIDVNPS RKVKGADEVI ERAYASLHVT HHDRPVAWAL 670 680 690 700 710 720 LPTASRSHTM ALATRPGVEP FTVLRNDATV QAVRSAGALL TKDPTVVTTL AFWKPATCGG 730 740 750 760 770 780 VAVNRPALVQ TRESANQMEV VIVEPTQKRG SLTVTIEGSW KVKTADSHVD VSCENAAGTL 790 800 810 HVDTAGLGGQ SVRVTLARQV TQTPSGGGRH DRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the hyaluronate lyase gene from Propionibacterium acnes." Steiner B.M., Romero-Steiner S., Cruce D., George R. Can. J. Microbiol. 43:315-321(1997) [PubMed: 9115089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The complete genome sequence of Propionibacterium acnes, a commensal of human skin." Brueggemann H., Henne A., Hoster F., Liesegang H., Wiezer A., Strittmatter A., Hujer S., Duerre P., Gottschalk G. Science 305:671-673(2004) [PubMed: 15286373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KPA171202 / DSM 16379.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15927 Genomic DNA. Translation: AAA51650.1. Frameshift. AE017283 Genomic DNA. Translation: AAT82132.1.
RefSeq	YP_055090.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL8. Polysaccharide Lyase Family 8.
Genome annotation databases
GeneID	2932015.
GenomeReviews	Gene locus PPA0380 in contig AE017283_GR.
KEGG	pac:PPA0380.
NMPDR	fig|267747.1.peg.367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59634.
OMA	Q59634. SRINESA.
Enzyme and pathway databases
BioCyc	PACN267747:PPA0380-MON.
BRENDA	4.2.2.1. 39123.
Family and domain databases
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR012970. Lyase_8_alpha_N. IPR004103. Lyase_8_C. IPR003159. Lyase_8_central. IPR012329. Lyase_8_N. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:2.60.220.10. Lyase_8_C. 1 hit. G3DSA:1.50.10.100. Lyase_8_N. 1 hit.
Pfam	PF02278. Lyase_8. 1 hit. PF02884. Lyase_8_C. 1 hit. PF08124. Lyase_8_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYSA_PROAC

Accession

Primary (citable) accession number: Q59634
Secondary accession number(s): Q6AAT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 27, 2004
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)