DNA-directed RNA polymerase subunit beta - Neisseria meningitidis serogroup B

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	NMB0132

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	1392 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.
Sequence caution	The sequence AAF40591.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Antibiotic resistance Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1392

1392

DNA-directed RNA polymerase subunit beta HAMAP MF_01321

PRO_0000047930

Natural variations

Natural variant

549

1

S → P Rifampicin resistant.

Experimental info

Sequence conflict

2

1

N → S in CAA91164. Ref.1

Sequence conflict

28

1

L → I in CAA91164. Ref.1

Sequence conflict

224 – 252

29

ILDIF…DRLKG → NLGYFLRQRNVLFVFKRCSN RFGRRPSES in CAA91164. Ref.1

Sequence conflict

284

1

N → L in CAA91164. Ref.1

Sequence conflict

350 – 360

11

AYISNTLRTDE → VISPIPCVRMK in CAA91164. Ref.1

Sequence conflict

378

1

Missing in CAA91164. Ref.1

Sequence conflict

648

1

A → G in CAA91164. Ref.1

Sequence conflict

706

1

A → P in CAA91164. Ref.1

Sequence conflict

717 – 718

2

VP → SA in CAA91164. Ref.1

Sequence conflict

748 – 749

2

GG → A in CAA91164. Ref.1

Sequence conflict

818

1

F → L in CAA91164. Ref.1

Sequence conflict

836 – 837

2

GY → VN in CAA91164. Ref.1

Sequence conflict

1190 – 1192

3

YNG → SR in CAA91164. Ref.1

Sequence conflict

1201

1

A → S in CAA91164. Ref.1

Sequence conflict

1244 – 1246

3

DDP → EDA in CAA91164. Ref.1

Sequence conflict

1373

1

F → L in CAA91164. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q59622 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 92AA331A597898F1
Show »

FASTA

1,392

155,710

        10         20         30         40         50         60 
MNYSFTEKKR IRKSFAKREN VLEVPFLLAT QIDSYAKFLQ LENAFDKRTD DGLQAAFNSI 

        70         80         90        100        110        120 
FPIVSHNGYA RLEFVHYTLG EPLFDIPECQ LRGITYAAPL RARIRLVILD KEASKPTVKE 

       130        140        150        160        170        180 
VRENEVYMGE IPLMTPSGSF VINGTERVIV SQLHRSPGVF FEHDKGKTHS SGKLLFSARI 

       190        200        210        220        230        240 
IPYRGSWLDF EFDPKDLLYF RIDRRRKMPV TILLKALGYN NEQILDIFYD KETFYLSSNG 

       250        260        270        280        290        300 
VQTDLVADRL KGETAKVDIL DKEGNVLVAK GKRITAKNIR DITNAGLTRL DVEPESLLGK 

       310        320        330        340        350        360 
ALAADLIDSE TGEVLASAND EITEELLAKF DINGVKEITT LYINELDQGA YISNTLRTDE 

       370        380        390        400        410        420 
TAGRQAARVA IYRMMRPGEP PTEEAVEQLF NRLFFSEDSY DLSRVGRMKF NTRTYEQKLS 

       430        440        450        460        470        480 
EAQQNSWYGR LLNETFAGAA DKGGYVLSVE DIVASIATLV ELRNGHGEVD DIDHLGNRRV 

       490        500        510        520        530        540 
RSVGELTENQ FRSGLARVER AVKERLNQAE SENLMPHDLI NAKPVSAAIK EFFGSSQLSQ 

       550        560        570        580        590        600 
FMDQTNPLSE VTHKRRVSAL GPGGLTRERA GFEVRDVHPT HYGRVCPIET PEGPNIGLIN 

       610        620        630        640        650        660 
SLSVYARTND YGFLETPYRR VIDGKVTEEI DYLSAIEEGR YVIAQANADL DSDGNLIGDL 

       670        680        690        700        710        720 
VTCREKGETI MATPDRVQYM DVATGQVVSV AASLIPFLEH DDANRALMGA NMQRQAVPCL 

       730        740        750        760        770        780 
RPEKPMVGTG IERSVAVDSA TAIVARRGGV VEYVDANRVV IRVHDDEATA GEVGVDIYNL 

       790        800        810        820        830        840 
VKFTRSNQST NINQRPAVKA GDVLQRGDLV ADGASTDFGE LALGQNMTIA FMPWNGYNYE 

       850        860        870        880        890        900 
DSILISEKVA ADDRYTSIHI EELNVVARDT KLGAEDITRD IPNLSERMQN RLDESGIVYI 

       910        920        930        940        950        960 
GAEVEAGDVL VGKVTPKGET QLTPEEKLLR AIFGEKASDV KDTSLRMPTG MSGTVIDVQV 

       970        980        990       1000       1010       1020 
FTREGIQRDK RAQSIIDSEL KRYRLDLNDQ LRIFDNDAFD RIERMIVGQK ANGGPMKLAK 

      1030       1040       1050       1060       1070       1080 
GSEITTEYLA GLPSRHDWFD IRLTDEDLAK QLELIKVSLQ QKREEADELY EIKKKKLTQG 

      1090       1100       1110       1120       1130       1140 
DELQPGVQKM VKVFIAIKRR LQAGDKMAGR HGNKGVVSRI LPVEDMPYMA DGRPVDIVLN 

      1150       1160       1170       1180       1190       1200 
PLGVPSRMNI GQILEVHLGW AAKGIGERID RMLKEQRKAG ELREFLNRLY NGSGKKEDLD 

      1210       1220       1230       1240       1250       1260 
ALTDEEIIEL ASNLRKGASF ASPVFDGAKE SEIREMLNLA YPSDDPEVEK LGFNDSKTQI 

      1270       1280       1290       1300       1310       1320 
TLYDGRSGEA FDRKVTVGVM HYLKLHHLVD EKMHARSTGP YSLVTQQPLG GKAQFGGQRF 

      1330       1340       1350       1360       1370       1380 
GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYENIVK GEHKIDAGMP ESFNVLVKEI 

      1390 
RSLGLDIDLE RY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rifampicin resistance in Neisseria meningitidis: evidence from a study of sibling strains, description of new mutations and notes on population genetics." Nolte O.J. J. Antimicrob. Chemother. 39:747-755(1997) [PubMed: 9222044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT RIFAMPICIN RESISTANT. Strain: BNCV / Serogroup B.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54353 Genomic DNA. Translation: CAA91164.1. AE002098 Genomic DNA. Translation: AAF40591.1. Different initiation.
PIR	A81236. T30824.
RefSeq	NP_273190.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q59622.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009127; EBNEIP00000008747; EBNEIG00000009127.
GeneID	902240.
GenomeReviews	Gene locus NMB0132 in contig AE002098_GR.
KEGG	nme:NMB0132.
NMPDR	fig\|122586.1.peg.124.
PATRIC	20355283. VBINeiMen85645_0171.
TIGR	NMB0132.
Phylogenomic databases
GeneTree	EBGT00050000021504.
HOGENOM	HBG285547.
OMA	VDARDTK.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0132-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_NEIMB

Accession

Primary (citable) accession number: Q59622

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents