Reviewed, UniProtKB/Swiss-Prot Q59606 (MTF7_NEIGO)

Names and origin

Protein names

Recommended name:
    Modification methylase NgoFVII
      Short name=M.NgoFVII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase NgoFVII
      Short name=M.NgoVII

Gene names

Name:	ngoFVIIM
Synonyms:	dcmG

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Modification methylase NgoFVII		PRO_0000087898
Sites
	Active site	88	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59606-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6210BA089D906894	FASTA	374	42,073
	10 20 30 40 50 60 MLSKQISNLN SSSNKPKILS LFSGCGGLDL GFHQAGCETV WANDFSHWAC ESFRKNIGDV 70 80 90 100 110 120 IVEGDIEQIN PNDPTIPDCD IILGGFPCQD FSMIWKQPGL EGERGNLYKS FLRFVNAKKP 130 140 150 160 170 180 KVFVAENVKG LLTANKKKAI QQIITDFENC GYYVQANVYN FAEFGVPQFR ERVLIVGVRL 190 200 210 220 230 240 DTGFDFRHPE PTHNETGENG LKPYVTAGQA ISNIPQNASN NELLKISDKT RRMLELIPEG 250 260 270 280 290 300 GNFTDIPKDH PLYVKGMISH VYRRMHRNEP SKTIIAAGGG GTWGYHFPEP RAFTNRERAR 310 320 330 340 350 360 LQSFPDDFEF VGSTTEVRRQ IGNAVPPQGV VELAKSILPI FSDNYEKVDL HEKLVEEKEI 370 LFHDRLSKIR GGKQ

References

[1]	"Restriction and modification systems of Neisseria gonorrhoeae." Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A. Gene 157:19-22(1995) [PubMed: 7607490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1291C.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43736 Genomic DNA. Translation: AAA86270.1.
PIR	T10165.
3D structure databases
HSSP	HSSP built from PDB template 1DCT based on UniProtKB P20589.
ModBase	Search...
Protein family/group databases
REBASE	3611. M.NgoFVII.
Enzyme and pathway databases
BRENDA	2.1.1.37. 588.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTF7_NEIGO

Accession

Primary (citable) accession number: Q59606

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)