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Q59606 (MTF7_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NgoFVII

Short name=M.NgoFVII
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NgoFVII
Short name=M.NgoVII
Gene names
Name:ngoFVIIM
Synonyms:dcmG
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Modification methylase NgoFVII
PRO_0000087898

Regions

Domain16 – 344329SAM-dependent MTase C5-type

Sites

Active site881 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59606 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6210BA089D906894

FASTA37442,073
        10         20         30         40         50         60 
MLSKQISNLN SSSNKPKILS LFSGCGGLDL GFHQAGCETV WANDFSHWAC ESFRKNIGDV 

        70         80         90        100        110        120 
IVEGDIEQIN PNDPTIPDCD IILGGFPCQD FSMIWKQPGL EGERGNLYKS FLRFVNAKKP 

       130        140        150        160        170        180 
KVFVAENVKG LLTANKKKAI QQIITDFENC GYYVQANVYN FAEFGVPQFR ERVLIVGVRL 

       190        200        210        220        230        240 
DTGFDFRHPE PTHNETGENG LKPYVTAGQA ISNIPQNASN NELLKISDKT RRMLELIPEG 

       250        260        270        280        290        300 
GNFTDIPKDH PLYVKGMISH VYRRMHRNEP SKTIIAAGGG GTWGYHFPEP RAFTNRERAR 

       310        320        330        340        350        360 
LQSFPDDFEF VGSTTEVRRQ IGNAVPPQGV VELAKSILPI FSDNYEKVDL HEKLVEEKEI 

       370 
LFHDRLSKIR GGKQ 

« Hide

References

[1]"Restriction and modification systems of Neisseria gonorrhoeae."
Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.
Gene 157:19-22(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1291C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43736 Genomic DNA. Translation: AAA86270.1.
PIRT10165.

3D structure databases

ProteinModelPortalQ59606.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3611. M.NgoFVII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTF7_NEIGO
AccessionPrimary (citable) accession number: Q59606
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries