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Q59605 (MTB5_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NgoBV

Short name=M.NgoBV
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NgoBV
Short name=M.NgoV
Gene names
Name:ngoBVM
Synonyms:dcmE
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Modification methylase NgoBV
PRO_0000087897

Regions

Domain4 – 423420SAM-dependent MTase C5-type

Sites

Active site801 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59605 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DFB0377B3E21480E

FASTA42347,571
        10         20         30         40         50         60 
MQQIKFIDLF SGMSGIRKGF EQACRKQSVA CECVFTSEIK PAALEVLKQN YPDEVPYGDI 

        70         80         90        100        110        120 
TKIETGDIPD FDILLAGFPC QAFSFAGKRL GFEDTRGTLF FDVARILKAK KPKGFILENV 

       130        140        150        160        170        180 
EGLVTHDRKD STQKIGRTLT VILETLEALG YYVSWKVLNA KEFGIPQNRK RIYLTGSLKS 

       190        200        210        220        230        240 
KPDLSFETSP SPKLKNILES GLPTESSPFI KKLLKKFPPS ELYGKSVKDK RGGKNNIHSW 

       250        260        270        280        290        300 
DIELKGAVTE EEKQLLNILL KERRKKNGLQ KIGIDWMDGM PLTKAQISTF YKHPDLQNIL 

       310        320        330        340        350        360 
DSLTDKGYLV LEHPKQKIGG QRIKDESLPK GYNIVSGKKS FEINKILDPN DVAPTLFAMD 

       370        380        390        400        410        420 
MEHLFVVDNG GLRTLTGKEG LRLFGYPDDY PFDIPKKDRC DLLGNTVAVP VIKAVSERLL 


HTL 

« Hide

References

[1]"Restriction and modification systems of Neisseria gonorrhoeae."
Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.
Gene 157:19-22(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: WR302.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43735 Genomic DNA. Translation: AAA86268.1.

3D structure databases

ProteinModelPortalQ59605.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3608. M.NgoBV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB5_NEIGO
AccessionPrimary (citable) accession number: Q59605
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries