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Protein

Modification methylase NgoBV

Gene

ngoBVM

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3608. M.NgoBV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NgoBV (EC:2.1.1.37)
Short name:
M.NgoBV
Alternative name(s):
Cytosine-specific methyltransferase NgoBV
Short name:
M.NgoV
Gene namesi
Name:ngoBVM
Synonyms:dcmE
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Modification methylase NgoBVPRO_0000087897Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi528354.NGFG_01121.

Structurei

3D structure databases

ProteinModelPortaliQ59605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 423420SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQIKFIDLF SGMSGIRKGF EQACRKQSVA CECVFTSEIK PAALEVLKQN
60 70 80 90 100
YPDEVPYGDI TKIETGDIPD FDILLAGFPC QAFSFAGKRL GFEDTRGTLF
110 120 130 140 150
FDVARILKAK KPKGFILENV EGLVTHDRKD STQKIGRTLT VILETLEALG
160 170 180 190 200
YYVSWKVLNA KEFGIPQNRK RIYLTGSLKS KPDLSFETSP SPKLKNILES
210 220 230 240 250
GLPTESSPFI KKLLKKFPPS ELYGKSVKDK RGGKNNIHSW DIELKGAVTE
260 270 280 290 300
EEKQLLNILL KERRKKNGLQ KIGIDWMDGM PLTKAQISTF YKHPDLQNIL
310 320 330 340 350
DSLTDKGYLV LEHPKQKIGG QRIKDESLPK GYNIVSGKKS FEINKILDPN
360 370 380 390 400
DVAPTLFAMD MEHLFVVDNG GLRTLTGKEG LRLFGYPDDY PFDIPKKDRC
410 420
DLLGNTVAVP VIKAVSERLL HTL
Length:423
Mass (Da):47,571
Last modified:November 1, 1996 - v1
Checksum:iDFB0377B3E21480E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43735 Genomic DNA. Translation: AAA86268.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43735 Genomic DNA. Translation: AAA86268.1.

3D structure databases

ProteinModelPortaliQ59605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi528354.NGFG_01121.

Protein family/group databases

REBASEi3608. M.NgoBV.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Restriction and modification systems of Neisseria gonorrhoeae."
    Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.
    Gene 157:19-22(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WR302.

Entry informationi

Entry nameiMTB5_NEIGO
AccessioniPrimary (citable) accession number: Q59605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.