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Q59603

- MTB1_NEIGO

UniProt

Q59603 - MTB1_NEIGO

Protein

Modification methylase NgoBI

Gene

ngoBIM

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (01 May 2000)
      Previous versions | rss
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    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3607. M.NgoBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase NgoBI (EC:2.1.1.37)
    Short name:
    M.NgoBI
    Alternative name(s):
    Cytosine-specific methyltransferase NgoBI
    Short name:
    M.NgoI
    Gene namesi
    Name:ngoBIM
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Modification methylase NgoBIPRO_0000087896Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ59603.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 302301SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59603-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKTIDLFSG IGGIRLGFEK YGCTNVFSSE WDKYARQVYE ANFGEKPFGD    50
    INGIDPSDIP DHDILLAGFP CQPFSIAGKG LGFEDTRGTL FFNIAEILKT 100
    KQPKAFLLEN VKRLTTHDSG RTFRIVLETL KQLGYTVYFK VLNTLDFGLP 150
    QKRERIYIVG FSDNIPFYFP EPINQYRPLG ELLENDRDVE PSYFLSDTLK 200
    QKRLAALKKA PPTPSIWHEN IGGNVSALPY SCALRAGGSY NYLVVNGVRR 250
    LTGREMLRLQ GFPDDFEINI PYSQVRKVAG NSVSVPVIEA TRKICSLLFP 300
    ARSNKKGNWI YWRQDDA 317
    Length:317
    Mass (Da):35,949
    Last modified:May 1, 2000 - v2
    Checksum:iF0549A5259D1A414
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42459 Genomic DNA. Translation: AAB03206.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42459 Genomic DNA. Translation: AAB03206.2 .

    3D structure databases

    ProteinModelPortali Q59603.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3607. M.NgoBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
      Stein D.C., Gunn J.S., Piekarowicz A.
      Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: WR302.

    Entry informationi

    Entry nameiMTB1_NEIGO
    AccessioniPrimary (citable) accession number: Q59603
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3