Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q59603 (MTB1_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NgoBI

Short name=M.NgoBI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NgoBI
Short name=M.NgoI
Gene names
Name:ngoBIM
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Modification methylase NgoBI
PRO_0000087896

Regions

Domain2 – 302301SAM-dependent MTase C5-type

Sites

Active site711 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59603 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: F0549A5259D1A414

FASTA31735,949
        10         20         30         40         50         60 
MYKTIDLFSG IGGIRLGFEK YGCTNVFSSE WDKYARQVYE ANFGEKPFGD INGIDPSDIP 

        70         80         90        100        110        120 
DHDILLAGFP CQPFSIAGKG LGFEDTRGTL FFNIAEILKT KQPKAFLLEN VKRLTTHDSG 

       130        140        150        160        170        180 
RTFRIVLETL KQLGYTVYFK VLNTLDFGLP QKRERIYIVG FSDNIPFYFP EPINQYRPLG 

       190        200        210        220        230        240 
ELLENDRDVE PSYFLSDTLK QKRLAALKKA PPTPSIWHEN IGGNVSALPY SCALRAGGSY 

       250        260        270        280        290        300 
NYLVVNGVRR LTGREMLRLQ GFPDDFEINI PYSQVRKVAG NSVSVPVIEA TRKICSLLFP 

       310 
ARSNKKGNWI YWRQDDA 

« Hide

References

[1]"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
Stein D.C., Gunn J.S., Piekarowicz A.
Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: WR302.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U42459 Genomic DNA. Translation: AAB03206.2.

3D structure databases

ProteinModelPortalQ59603.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3607. M.NgoBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_NEIGO
AccessionPrimary (citable) accession number: Q59603
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries