Q59603 (MTB1_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase NgoBI
Short name=M.NgoBI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase NgoBI
Short name=M.NgoI

Gene names

Name:

ngoBIM

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Modification methylase NgoBI		PRO_0000087896
Sites
	Active site	71	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59603 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: F0549A5259D1A414	FASTA	317	35,949
	10 20 30 40 50 60 MYKTIDLFSG IGGIRLGFEK YGCTNVFSSE WDKYARQVYE ANFGEKPFGD INGIDPSDIP 70 80 90 100 110 120 DHDILLAGFP CQPFSIAGKG LGFEDTRGTL FFNIAEILKT KQPKAFLLEN VKRLTTHDSG 130 140 150 160 170 180 RTFRIVLETL KQLGYTVYFK VLNTLDFGLP QKRERIYIVG FSDNIPFYFP EPINQYRPLG 190 200 210 220 230 240 ELLENDRDVE PSYFLSDTLK QKRLAALKKA PPTPSIWHEN IGGNVSALPY SCALRAGGSY 250 260 270 280 290 300 NYLVVNGVRR LTGREMLRLQ GFPDDFEINI PYSQVRKVAG NSVSVPVIEA TRKICSLLFP 310 ARSNKKGNWI YWRQDDA

References

[1]	"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems." Stein D.C., Gunn J.S., Piekarowicz A. Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WR302.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U42459 Genomic DNA. Translation: AAB03206.2.
3D structure databases
ProteinModelPortal	Q59603.
ModBase	Search...
Protein family/group databases
REBASE	3607. M.NgoBI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_NEIGO

Accession

Primary (citable) accession number: Q59603

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program