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Protein

Modification methylase NgoBI

Gene

ngoBIM

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3607. M.NgoBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NgoBI (EC:2.1.1.37)
Short name:
M.NgoBI
Alternative name(s):
Cytosine-specific methyltransferase NgoBI
Short name:
M.NgoI
Gene namesi
Name:ngoBIM
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Modification methylase NgoBIPRO_0000087896Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ59603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 302301SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKTIDLFSG IGGIRLGFEK YGCTNVFSSE WDKYARQVYE ANFGEKPFGD
60 70 80 90 100
INGIDPSDIP DHDILLAGFP CQPFSIAGKG LGFEDTRGTL FFNIAEILKT
110 120 130 140 150
KQPKAFLLEN VKRLTTHDSG RTFRIVLETL KQLGYTVYFK VLNTLDFGLP
160 170 180 190 200
QKRERIYIVG FSDNIPFYFP EPINQYRPLG ELLENDRDVE PSYFLSDTLK
210 220 230 240 250
QKRLAALKKA PPTPSIWHEN IGGNVSALPY SCALRAGGSY NYLVVNGVRR
260 270 280 290 300
LTGREMLRLQ GFPDDFEINI PYSQVRKVAG NSVSVPVIEA TRKICSLLFP
310
ARSNKKGNWI YWRQDDA
Length:317
Mass (Da):35,949
Last modified:April 30, 2000 - v2
Checksum:iF0549A5259D1A414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42459 Genomic DNA. Translation: AAB03206.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42459 Genomic DNA. Translation: AAB03206.2.

3D structure databases

ProteinModelPortaliQ59603.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3607. M.NgoBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
    Stein D.C., Gunn J.S., Piekarowicz A.
    Biol. Chem. 379:575-578(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WR302.

Entry informationi

Entry nameiMTB1_NEIGO
AccessioniPrimary (citable) accession number: Q59603
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: April 30, 2000
Last modified: January 6, 2015
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.