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Protein

Fimbrial protein

Gene

pilA

Organism
Myxococcus xanthus (strain DK 1622)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Makes up the pili which are long filamentous (less than 10 nm thick and up to 10 um long) polarly located appendages. They are required for social (S) motility.1 Publication

Enzyme and pathway databases

BioCyciMXAN246197:GIWU-5740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fimbrial protein
Alternative name(s):
Pilin
Gene namesi
Name:pilA
Ordered Locus Names:MXAN_5783
OrganismiMyxococcus xanthus (strain DK 1622)
Taxonomic identifieri246197 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus
Proteomesi
  • UP000002402 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • pilus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491K → E: Pilus-minus S phenotype. 1 Publication
Mutagenesisi193 – 1931G → D: Pilus-minus S phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1212By similarityPRO_0000246780Add
BLAST
Chaini13 – 220208Fimbrial proteinPRO_0000246781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131N-methylphenylalaninePROSITE-ProRule annotation

Keywords - PTMi

Methylation

Expressioni

Inductioni

Expression of pilA seems to be negatively regulated by the accumulation of pilin at a cell end. This may be sensed by the PilR-PilS two-component system.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi246197.MXAN_5783.

Structurei

3D structure databases

ProteinModelPortaliQ59589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N-Me-Phe pilin family.Curated

Phylogenomic databases

eggNOGiENOG4106855. Bacteria.
ENOG410XUCS. LUCA.
HOGENOMiHOG000115547.
KOiK02650.
OMAiWVIAGFE.
OrthoDBiEOG6NWBPW.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR028188. Pilin_PilA.
[Graphical view]
PfamiPF13633. N_methyl_3. 1 hit.
PF14245. Pilin_PilA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVSRFNPRN RGFTLIELMI VVAIIGILAA IAIPNFIKFQ ARSKQSEAKT
60 70 80 90 100
NLKALYTAQK SFFSEKDRYS DFANEIGFAP ERGNRYGYRV SAAAGDCEVR
110 120 130 140 150
NAADLPVPAA GVPCISNDSF RFGANSAIDD PTPVVARFVP QGAAGWNTTL
160 170 180 190 200
GVQPTIADCP NCNFFAGARG NADNEATFDD WVIAGFEGSG QVGPCSEAGN
210 220
VASGTPYNTR NDVACDGAAQ
Length:220
Mass (Da):23,388
Last modified:November 1, 1996 - v1
Checksum:i20B7B699C74DE3DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39904 Genomic DNA. Translation: AAB40882.1.
CP000113 Genomic DNA. Translation: ABF90139.1.
PIRiS70530.
RefSeqiWP_011555734.1. NC_008095.1.

Genome annotation databases

EnsemblBacteriaiABF90139; ABF90139; MXAN_5783.
KEGGimxa:MXAN_5783.
PATRICi22654085. VBIMyxXan43560_5687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39904 Genomic DNA. Translation: AAB40882.1.
CP000113 Genomic DNA. Translation: ABF90139.1.
PIRiS70530.
RefSeqiWP_011555734.1. NC_008095.1.

3D structure databases

ProteinModelPortaliQ59589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246197.MXAN_5783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF90139; ABF90139; MXAN_5783.
KEGGimxa:MXAN_5783.
PATRICi22654085. VBIMyxXan43560_5687.

Phylogenomic databases

eggNOGiENOG4106855. Bacteria.
ENOG410XUCS. LUCA.
HOGENOMiHOG000115547.
KOiK02650.
OMAiWVIAGFE.
OrthoDBiEOG6NWBPW.

Enzyme and pathway databases

BioCyciMXAN246197:GIWU-5740-MONOMER.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR028188. Pilin_PilA.
[Graphical view]
PfamiPF13633. N_methyl_3. 1 hit.
PF14245. Pilin_PilA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and functional evidence that type IV pili are required for social gliding motility in Myxococcus xanthus."
    Wu S.S., Kaiser D.
    Mol. Microbiol. 18:547-558(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF LYS-49 AND GLY-193.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DK 1622.
  3. "Regulating pilin expression reveals a threshold for S motility in Myxococcus xanthus."
    Jelsbak L., Kaiser D.
    J. Bacteriol. 187:2105-2112(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION, INDUCTION.

Entry informationi

Entry nameiPILA_MYXXD
AccessioniPrimary (citable) accession number: Q59589
Secondary accession number(s): Q1D0A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Pilin assembly limits pilus production and S motility. This limitation could be linked to the high frequency of reversal of gliding direction observed in M.xanthus. Reversal implies loss of pili at the leading pole of the cell and commissioning of new pili assembly at the other pole.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.