Q59587 (RISC_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
EC=2.5.1.9

Gene names

Name:	ribC
Ordered Locus Names:	MTBMA_c05840

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanothermobacter marburgensis

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The relatively low activity of this enzyme suggested that 6,7-dimethyl-8-ribityllumazine might not be its natural substrate.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by EDTA.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Homooligomer.
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Riboflavin synthase		PRO_0000134862

Sequences

	Sequence		Length	Mass (Da)
	Q59587 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CAAE34FDA348F534	FASTA	153	16,969
	10 20 30 40 50 60 MIKVGICDTT FARYDMGGAA IDEIKKHATG IKIIRRTVPG IKDLPVACKK LIEEEGCEMV 70 80 90 100 110 120 MALGMPGPEE KDKVCAHEAS TGLIQAQLMT NTHILEVFVH EDEEDDPEDL KVLADNRARE 130 140 150 HAQNLIMMLF RPERLTRDAG MGMREGKPDV GPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum." Eberhardt S., Korn S., Lottspeich F., Bacher A. J. Bacteriol. 179:2938-2943(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-42 AND 129-151. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94292 Genomic DNA. Translation: CAA63959.1. CP001710 Genomic DNA. Translation: ADL58179.1.
RefSeq	YP_003849492.1. NC_014408.1.
3D structure databases
ProteinModelPortal	Q59587.
SMR	Q59587. Positions 3-152.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADL58179; ADL58179; MTBMA_c05840.
GeneID	9704292.
KEGG	mmg:MTBMA_c05840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000227234.
KO	K00793.
OMA	IVDTTFA.
ProtClustDB	PRK06455.
Enzyme and pathway databases
BioCyc	MMAR79929:GH5J-587-MONOMER.
BRENDA	2.5.1.9. 3256.
UniPathway	UPA00275; UER00405.
Family and domain databases
Gene3D	3.40.50.960. 1 hit.
InterPro	IPR002180. DMRL_synthase. IPR006399. Ribfl_synth_arc. [Graphical view]
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF015750. Ribfl_synth_arc. 1 hit.
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR01506. ribC_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISC_METTM

Accession

Primary (citable) accession number: Q59587
Secondary accession number(s): D9PVD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program