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Protein

Riboflavin synthase

Gene

ribC

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The relatively low activity of this enzyme suggested that 6,7-dimethyl-8-ribityllumazine might not be its natural substrate.

Catalytic activityi

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.

Cofactori

Enzyme regulationi

Inhibited by EDTA.

Pathway:iriboflavin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribH)
  2. Riboflavin synthase (ribC)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-587-MONOMER.
BRENDAi2.5.1.9. 3256.
UniPathwayiUPA00275; UER00405.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin synthase (EC:2.5.1.9)
Gene namesi
Name:ribC
Ordered Locus Names:MTBMA_c05840
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Riboflavin synthasePRO_0000134862Add
BLAST

Interactioni

Subunit structurei

Homooligomer.

Protein-protein interaction databases

STRINGi79929.MTBMA_c05840.

Structurei

3D structure databases

ProteinModelPortaliQ59587.
SMRiQ59587. Positions 3-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DMRL synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000227234.
KOiK00793.
OMAiGIVDTTF.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
InterProiIPR002180. DMRL_synthase.
IPR006399. Ribfl_synth_arc.
[Graphical view]
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF015750. Ribfl_synth_arc. 1 hit.
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR01506. ribC_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVGICDTT FARYDMGGAA IDEIKKHATG IKIIRRTVPG IKDLPVACKK
60 70 80 90 100
LIEEEGCEMV MALGMPGPEE KDKVCAHEAS TGLIQAQLMT NTHILEVFVH
110 120 130 140 150
EDEEDDPEDL KVLADNRARE HAQNLIMMLF RPERLTRDAG MGMREGKPDV

GPL
Length:153
Mass (Da):16,969
Last modified:November 1, 1996 - v1
Checksum:iCAAE34FDA348F534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94292 Genomic DNA. Translation: CAA63959.1.
CP001710 Genomic DNA. Translation: ADL58179.1.
RefSeqiWP_013295403.1. NC_014408.1.
YP_003849492.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL58179; ADL58179; MTBMA_c05840.
GeneIDi9704292.
KEGGimmg:MTBMA_c05840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94292 Genomic DNA. Translation: CAA63959.1.
CP001710 Genomic DNA. Translation: ADL58179.1.
RefSeqiWP_013295403.1. NC_014408.1.
YP_003849492.1. NC_014408.1.

3D structure databases

ProteinModelPortaliQ59587.
SMRiQ59587. Positions 3-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi79929.MTBMA_c05840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL58179; ADL58179; MTBMA_c05840.
GeneIDi9704292.
KEGGimmg:MTBMA_c05840.

Phylogenomic databases

HOGENOMiHOG000227234.
KOiK00793.
OMAiGIVDTTF.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00405.
BioCyciMMAR79929:GH5J-587-MONOMER.
BRENDAi2.5.1.9. 3256.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
InterProiIPR002180. DMRL_synthase.
IPR006399. Ribfl_synth_arc.
[Graphical view]
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF015750. Ribfl_synth_arc. 1 hit.
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR01506. ribC_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum."
    Eberhardt S., Korn S., Lottspeich F., Bacher A.
    J. Bacteriol. 179:2938-2943(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-42 AND 129-151.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiRISC_METTM
AccessioniPrimary (citable) accession number: Q59587
Secondary accession number(s): D9PVD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.