Q59586 (RECF_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication and repair protein recF

Gene names

Name:	recF
Ordered Locus Names:	Rv0003, MT0003
ORF Names:	MTCY10H4.01

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP By similarity. HAMAP MF_00365
Subcellular location	Cytoplasm Potential HAMAP MF_00365.
Sequence similarities	Belongs to the recF family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW single-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	DNA replication and repair protein recF HAMAP MF_00365		PRO_0000196435
Regions
	Nucleotide binding	30 – 37	8	ATP Potential
Experimental info
	Sequence conflict	245	1	I → T in AAK44226. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q59586 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 461D6480438ADBD7	FASTA	385	42,212
	10 20 30 40 50 60 MYVRHLGLRD FRSWACVDLE LHPGRTVFVG PNGYGKTNLI EALWYSTTLG SHRVSADLPL 70 80 90 100 110 120 IRVGTDRAVI STIVVNDGRE CAVDLEIATG RVNKARLNRS SVRSTRDVVG VLRAVLFAPE 130 140 150 160 170 180 DLGLVRGDPA DRRRYLDDLA IVRRPAIAAV RAEYERVLRQ RTALLKSVPG ARYRGDRGVF 190 200 210 220 230 240 DTLEVWDSRL AEHGAELVAA RIDLVNQLAP EVKKAYQLLA PESRSASIGY RASMDVTGPS 250 260 270 280 290 300 EQSDIDRQLL AARLLAALAA RRDAELERGV CLVGPHRDDL ILRLGDQPAK GFASHGEAWS 310 320 330 340 350 360 LAVALRLAAY QLLRVDGGEP VLLLDDVFAE LDVMRRRALA TAAESAEQVL VTAAVLEDIP 370 380 AGWDARRVHI DVRADDTGSM SVVLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis." Salazar L., Fsihi H., De Rossi E., Riccardi G., Rios C., Cole S.T., Takiff H.E. Mol. Microbiol. 20:283-293(1996) [PubMed: 8733228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92504 Genomic DNA. Translation: CAA63259.1. BX842572 Genomic DNA. Translation: CAB02424.1. AE000516 Genomic DNA. Translation: AAK44226.1.
PIR	S70984.
RefSeq	NP_214517.1. NC_000962.2. NP_334412.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q59586.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003667; EBMYCP00000003667; EBMYCG00000003665. EBMYCT00000070923; EBMYCP00000068982; EBMYCG00000070918.
GeneID	887089. 922431.
GenomeReviews	Gene locus MT0003 in contig AE000516_GR. Gene locus Rv0003 in contig AL123456_GR.
KEGG	mtc:MT0003. mtu:Rv0003.
PATRIC	18121730. VBIMycTub22151_0003.
TIGR	MT0003.
Organism-specific databases
TubercuList	Rv0003.
Phylogenomic databases
GeneTree	EBGT00050000017189.
HOGENOM	HBG645124.
OMA	GQQRTLV.
PhylomeDB	Q59586.
ProtClustDB	PRK00064.
Family and domain databases
HAMAP	MF_00365. RecF. [Tree]
InterPro	IPR001238. DNA-binding_RecF. IPR018078. DNA-binding_RecF_CS. IPR003395. RecF/RecN/SMC. [Graphical view]
KO	K03629.
Pfam	PF02463. SMC_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00611. Recf. 1 hit.
PROSITE	PS00617. RECF_1. 1 hit. PS00618. RECF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECF_MYCTU

Accession

Primary (citable) accession number: Q59586
Secondary accession number(s): P77896

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program