Q59579 (FWDC_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C
EC=1.2.99.5

Alternative name(s):
Tungsten-containing formylmethanofuran dehydrogenase II subunit C

Gene names

Name:	fwdC
Ordered Locus Names:	MTBMA_c01440

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize formylmethanofuran. This enzyme is oxygen-labile.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Tungsten.
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.
Subunit structure	This enzyme is composed of seven subunits fwdA (65 kDa), fwdB (53 kDa), fwdC (31 kDa), fwdD (15 kDa), fwdE, fwdF and fwdG By similarity.
Induction	By growth on tungsten or molybdenum under anaerobic conditions.
Sequence similarities	Belongs to the fwdC/fmdC family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Tungsten
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C		PRO_0000144197
Regions
	Repeat	80 – 92	13	1
	Repeat	99 – 111	13	2
	Repeat	118 – 130	13	3
	Repeat	144 – 156	13	4
	Repeat	163 – 175	13	5
	Repeat	182 – 194	13	6
	Repeat	201 – 213	13	7
	Region	80 – 213	134	7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-[IL]-X-[IV]-X-G
Experimental info
	Sequence conflict	223	1	K → T in CAA61214. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59579 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: DBCEC802ACFEC476	FASTA	270	28,582
	10 20 30 40 50 60 MSEIILTPKE QPEVPLEAPN IKPDVFAGKS IDEIKNIQIM YGNEVVKLGD FFEVSGEPAD 70 80 90 100 110 120 AASDIKIIID GDVYNTKRIG QEMTAGEILV KGNVNMYVGA GMKGGRITVE GNAASWAGQD 130 140 150 160 170 180 MRGGELEILG NAADYVGSSY RGDWRGMSGG VITVHGNAGN EIGEYMNGGK IIIKGDVNIM 190 200 210 220 230 240 PGIHMNNGLI IIEGNAVARV GGEMAGGTII VKGMIQEFLP GFKYLGVEKD IEVNGETFPG 250 260 270 AFYKFEGDHA IKGAKGIVYA AVGCNGHIEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide." Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R. Eur. J. Biochem. 234:910-920(1995) [PubMed: 8575452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87970 Genomic DNA. Translation: CAA61214.1. CP001710 Genomic DNA. Translation: ADL57753.1.
RefSeq	YP_003849066.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.8.1.1. Na+- or H+-pumping formyl methanofuran dehydrogenase (FMF-DH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9703849.
GenomeReviews	Gene locus MTBMA_c01440 in contig CP001710_GR.
KEGG	mmg:MTBMA_c01440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GNADMYV.
Family and domain databases
InterPro	IPR017550. Formylmethanofuran_DH_suC. IPR002489. Glu_synth_asu_C. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
KO	K00202.
Pfam	PF01493. GXGXG. 1 hit. [Graphical view]
SUPFAM	SSF69336. Glu_synthase_C. 1 hit.
TIGRFAMs	TIGR03122. One_C_dehyd_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FWDC_METTM

Accession

Primary (citable) accession number: Q59579
Secondary accession number(s): D9PU55, O08493

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 30, 2010
Last modified:	November 16, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program