Q59568 (PPGK_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyphosphate glucokinase
EC=2.7.1.63

Alternative name(s):
Polyphosphate--glucose phosphotransferase

Gene names

Name:	ppgK
Ordered Locus Names:	Rv2702, MT2776
ORF Names:	MTCY05A6.23

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. GTP, UTP and CTP can replace ATP as phosphoryl donor.
Catalytic activity	(Phosphate)(n) + D-glucose = (phosphate)(n-1) + D-glucose 6-phosphate.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	Was identified as a high-confidence drug target.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glucokinase activity Inferred from direct assay Ref.1. Source: MTBBASE polyphosphate-glucose phosphotransferase activity Inferred from direct assay Ref.1. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Polyphosphate glucokinase		PRO_0000058536
Regions
	Nucleotide binding	24 – 29	6	ATP Potential
Natural variations
	Natural variant	140	1	N → S in CDC 1551. Ref.3
Experimental info
	Sequence conflict	203	1	I → T in AAC43638. Ref.1
	Sequence conflict	203	1	I → T in AAK47091. Ref.3
	Sequence conflict	238	1	R → C in AAC43638. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59568 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 2F4AFAFAAC3C01E5	FASTA	265	27,429
	10 20 30 40 50 60 MTSTGPETSE TPGATTQRHG FGIDVGGSGI KGGIVDLDTG QLIGDRIKLL TPQPATPLAV 70 80 90 100 110 120 AKTIAEVVNG FGWRGPLGVT YPGVVTHGVV RTAANVDKSW IGTNARDTIG AELGGQQVTI 130 140 150 160 170 180 LNDADAAGLA ETRYGAGKNN PGLVVLLTFG TGIGSAVIHN GTLIPNTEFG HLEVGGKEAE 190 200 210 220 230 240 ERAASSVKEK NDWTYPKWAK QVIRVLIAIE NAIWPDLFIA GGGISRKADK WVPLLENRTP 250 260 VVPAALQNTA GIVGAAMASV ADTTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis." Hsieh P.-C., Shenoy B.C., Samols D., Phillips N.F.B. J. Biol. Chem. 271:4909-4915(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT SER-140. Strain: CDC 1551 / Oshkosh.
[4]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U44834 Genomic DNA. Translation: AAC43638.1. BX842580 Genomic DNA. Translation: CAB09462.1. AE000516 Genomic DNA. Translation: AAK47091.1. AL123456 Genomic DNA. Translation: CCP45500.1.
PIR	A70531.
RefSeq	NP_217218.1. NC_000962.3. NP_337277.1. NC_002755.2. YP_006516146.1. NC_018143.1.
3D structure databases
ProteinModelPortal	Q59568.
SMR	Q59568. Positions 35-257.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv2702.
Proteomic databases
PRIDE	Q59568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47091; AAK47091; MT2776.
GeneID	13319429. 887313. 925524.
KEGG	mtc:MT2776. mtu:Rv2702. mtv:RVBD_2702.
PATRIC	18127846. VBIMycTub22151_3022.
Organism-specific databases
TubercuList	Rv2702.
Phylogenomic databases
eggNOG	COG1940.
HOGENOM	HOG000150086.
KO	K00886.
OMA	AANVDKG.
ProtClustDB	CLSK792017.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-6062.
Family and domain databases
InterPro	IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPGK_MYCTU

Accession

Primary (citable) accession number: Q59568
Secondary accession number(s): L0TAD9, O07204

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 15, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program