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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961Phosphate1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 766ATP2 Publications
Nucleotide bindingi102 – 1054ATP2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2723-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecAUniRule annotation
Alternative name(s):
Recombinase AUniRule annotation
Gene namesi
Name:recAUniRule annotation
Ordered Locus Names:MSMEG_2723, MSMEI_2656
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential in the absence of DNA damage, its deletion renders cells much more sensitive to DNA damaging agents such as UV light, ionizing radiation, alkylating agents and DNA gyrase inhibitors and desiccation-induced DSBs. 5-fold decrease in survival, loss of HR, no change in NHEJ (non-homologous end-joining) or single-strand annealing DSB repair.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi196 – 1961Q → A, E or N: Loss of residue movement, loss of switch function in crystal structures. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Protein RecAPRO_0000122767Add
BLAST

Proteomic databases

PRIDEiQ59560.

Interactioni

Subunit structurei

Polymerizes non-specifically on ssDNA to form filaments. Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-215717.
STRINGi246196.MSMEG_2723.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2316Combined sources
Beta strandi25 – 284Combined sources
Helixi47 – 526Combined sources
Beta strandi54 – 596Combined sources
Beta strandi62 – 698Combined sources
Beta strandi70 – 734Combined sources
Helixi74 – 8714Combined sources
Beta strandi92 – 987Combined sources
Helixi103 – 1086Combined sources
Helixi113 – 1153Combined sources
Beta strandi117 – 1193Combined sources
Helixi124 – 13512Combined sources
Turni136 – 1383Combined sources
Beta strandi141 – 1466Combined sources
Helixi148 – 1503Combined sources
Helixi154 – 1574Combined sources
Beta strandi162 – 1643Combined sources
Helixi168 – 18720Combined sources
Beta strandi190 – 1967Combined sources
Beta strandi200 – 2023Combined sources
Beta strandi205 – 2073Combined sources
Beta strandi211 – 2133Combined sources
Helixi214 – 2207Combined sources
Beta strandi222 – 23312Combined sources
Beta strandi235 – 2384Combined sources
Beta strandi241 – 25414Combined sources
Beta strandi259 – 2657Combined sources
Beta strandi268 – 2703Combined sources
Helixi272 – 28211Combined sources
Beta strandi285 – 2895Combined sources
Beta strandi292 – 2954Combined sources
Beta strandi298 – 3036Combined sources
Helixi304 – 31310Combined sources
Helixi315 – 32713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UBCX-ray3.80A1-349[»]
1UBEX-ray3.30A1-349[»]
1UBFX-ray3.50A1-349[»]
1UBGX-ray3.50A1-349[»]
2G88X-ray3.20A1-349[»]
2ODNX-ray3.10A1-349[»]
2ODWX-ray3.30A1-349[»]
2OE2X-ray3.45A1-349[»]
2OEPX-ray3.10A1-349[»]
2OESX-ray3.50A1-349[»]
2OFOX-ray3.16A1-349[»]
2ZR0X-ray3.00A1-349[»]
2ZR7X-ray3.60A1-349[»]
2ZR9X-ray2.50A1-349[»]
2ZRAX-ray3.10A1-349[»]
2ZRBX-ray3.25A1-349[»]
2ZRCX-ray3.10A1-349[»]
2ZRDX-ray3.10A1-349[»]
2ZREX-ray2.90A1-349[»]
2ZRFX-ray3.00A1-349[»]
2ZRGX-ray3.50A1-349[»]
2ZRHX-ray3.20A1-349[»]
2ZRIX-ray3.30A1-349[»]
2ZRJX-ray2.60A1-349[»]
2ZRKX-ray3.20A1-349[»]
2ZRLX-ray3.70A1-349[»]
2ZRMX-ray2.80A1-349[»]
2ZRNX-ray3.30A1-349[»]
2ZROX-ray2.90A1-349[»]
2ZRPX-ray3.30A1-349[»]
ProteinModelPortaliQ59560.
SMRiQ59560. Positions 1-330.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59560.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 755Phosphate binding1 Publication

Sequence similaritiesi

Belongs to the RecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C68. Bacteria.
COG0468. LUCA.
HOGENOMiHOG000264120.
KOiK03553.
OMAiPPFKEAH.
OrthoDBiPOG091H01OP.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQAPDREK ALELAMAQID KNFGKGSVMR LGEEVRQPIS VIPTGSISLD
60 70 80 90 100
VALGIGGLPR GRVIEIYGPE SSGKTTVALH AVANAQAAGG IAAFIDAEHA
110 120 130 140 150
LDPEYAKKLG VDTDSLLVSQ PDTGEQALEI ADMLVRSGAL DIIVIDSVAA
160 170 180 190 200
LVPRAEIEGE MGDSHVGLQA RLMSQALRKM TGALNNSGTT AIFINQLREK
210 220 230 240 250
IGVMFGSPET TTGGKALKFY ASVRLDVRRI ETLKDGTDAV GNRTRVKVVK
260 270 280 290 300
NKVSPPFKQA EFDILYGQGI SREGSLIDMG VEHGFIRKSG SWFTYEGEQL
310 320 330 340
GQGKENARKF LLENTDVANE IEKKIKEKLG IGAVVTAEAD DVLPAPVDF
Length:349
Mass (Da):37,301
Last modified:November 1, 1997 - v1
Checksum:iD85D21F5BFC5A020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99208 Genomic DNA. Translation: CAA67597.1.
CP000480 Genomic DNA. Translation: ABK74334.1.
CP001663 Genomic DNA. Translation: AFP39124.1.
RefSeqiWP_003894107.1. NZ_CP009494.1.
YP_887057.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74334; ABK74334; MSMEG_2723.
AFP39124; AFP39124; MSMEI_2656.
GeneIDi4532488.
KEGGimsb:LJ00_13540.
msg:MSMEI_2656.
msm:MSMEG_2723.
PATRICi18077876. VBIMycSme59918_2690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99208 Genomic DNA. Translation: CAA67597.1.
CP000480 Genomic DNA. Translation: ABK74334.1.
CP001663 Genomic DNA. Translation: AFP39124.1.
RefSeqiWP_003894107.1. NZ_CP009494.1.
YP_887057.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UBCX-ray3.80A1-349[»]
1UBEX-ray3.30A1-349[»]
1UBFX-ray3.50A1-349[»]
1UBGX-ray3.50A1-349[»]
2G88X-ray3.20A1-349[»]
2ODNX-ray3.10A1-349[»]
2ODWX-ray3.30A1-349[»]
2OE2X-ray3.45A1-349[»]
2OEPX-ray3.10A1-349[»]
2OESX-ray3.50A1-349[»]
2OFOX-ray3.16A1-349[»]
2ZR0X-ray3.00A1-349[»]
2ZR7X-ray3.60A1-349[»]
2ZR9X-ray2.50A1-349[»]
2ZRAX-ray3.10A1-349[»]
2ZRBX-ray3.25A1-349[»]
2ZRCX-ray3.10A1-349[»]
2ZRDX-ray3.10A1-349[»]
2ZREX-ray2.90A1-349[»]
2ZRFX-ray3.00A1-349[»]
2ZRGX-ray3.50A1-349[»]
2ZRHX-ray3.20A1-349[»]
2ZRIX-ray3.30A1-349[»]
2ZRJX-ray2.60A1-349[»]
2ZRKX-ray3.20A1-349[»]
2ZRLX-ray3.70A1-349[»]
2ZRMX-ray2.80A1-349[»]
2ZRNX-ray3.30A1-349[»]
2ZROX-ray2.90A1-349[»]
2ZRPX-ray3.30A1-349[»]
ProteinModelPortaliQ59560.
SMRiQ59560. Positions 1-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-215717.
STRINGi246196.MSMEG_2723.

Proteomic databases

PRIDEiQ59560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74334; ABK74334; MSMEG_2723.
AFP39124; AFP39124; MSMEI_2656.
GeneIDi4532488.
KEGGimsb:LJ00_13540.
msg:MSMEI_2656.
msm:MSMEG_2723.
PATRICi18077876. VBIMycSme59918_2690.

Phylogenomic databases

eggNOGiENOG4105C68. Bacteria.
COG0468. LUCA.
HOGENOMiHOG000264120.
KOiK03553.
OMAiPPFKEAH.
OrthoDBiPOG091H01OP.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2723-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ59560.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECA_MYCS2
AccessioniPrimary (citable) accession number: Q59560
Secondary accession number(s): A0QVW9, I7G989
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.