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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196Phosphate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 76ATP2 Publications6
Nucleotide bindingi102 – 105ATP2 Publications4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecAUniRule annotation
Alternative name(s):
Recombinase AUniRule annotation
Gene namesi
Name:recAUniRule annotation
Ordered Locus Names:MSMEG_2723, MSMEI_2656
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential in the absence of DNA damage, its deletion renders cells much more sensitive to DNA damaging agents such as UV light, ionizing radiation, alkylating agents and DNA gyrase inhibitors and desiccation-induced DSBs. 5-fold decrease in survival, loss of HR, no change in NHEJ (non-homologous end-joining) or single-strand annealing DSB repair.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi196Q → A, E or N: Loss of residue movement, loss of switch function in crystal structures. 1 Publication1

Chemistry databases

DrugBankiDB03222 2'-Deoxyadenosine 5'-Triphosphate
DB01660 Adenosine-5'-Diphosphate Monothiophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001227671 – 349Protein RecAAdd BLAST349

Proteomic databases

PRIDEiQ59560

Interactioni

Subunit structurei

Polymerizes non-specifically on ssDNA to form filaments. Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair (By similarity).By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2723

Structurei

Secondary structure

1349
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 23Combined sources16
Beta strandi25 – 28Combined sources4
Helixi47 – 52Combined sources6
Beta strandi54 – 59Combined sources6
Beta strandi62 – 69Combined sources8
Beta strandi70 – 73Combined sources4
Helixi74 – 87Combined sources14
Beta strandi92 – 98Combined sources7
Helixi103 – 108Combined sources6
Helixi113 – 115Combined sources3
Beta strandi117 – 119Combined sources3
Helixi124 – 135Combined sources12
Turni136 – 138Combined sources3
Beta strandi141 – 146Combined sources6
Helixi148 – 150Combined sources3
Helixi154 – 157Combined sources4
Beta strandi162 – 164Combined sources3
Helixi168 – 187Combined sources20
Beta strandi190 – 196Combined sources7
Beta strandi200 – 202Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi211 – 213Combined sources3
Helixi214 – 220Combined sources7
Beta strandi222 – 233Combined sources12
Beta strandi235 – 238Combined sources4
Beta strandi241 – 254Combined sources14
Beta strandi259 – 265Combined sources7
Beta strandi268 – 270Combined sources3
Helixi272 – 282Combined sources11
Beta strandi285 – 289Combined sources5
Beta strandi292 – 295Combined sources4
Beta strandi298 – 303Combined sources6
Helixi304 – 313Combined sources10
Helixi315 – 327Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UBCX-ray3.80A1-349[»]
1UBEX-ray3.30A1-349[»]
1UBFX-ray3.50A1-349[»]
1UBGX-ray3.50A1-349[»]
2G88X-ray3.20A1-349[»]
2ODNX-ray3.10A1-349[»]
2ODWX-ray3.30A1-349[»]
2OE2X-ray3.45A1-349[»]
2OEPX-ray3.10A1-349[»]
2OESX-ray3.50A1-349[»]
2OFOX-ray3.16A1-349[»]
2ZR0X-ray3.00A1-349[»]
2ZR7X-ray3.60A1-349[»]
2ZR9X-ray2.50A1-349[»]
2ZRAX-ray3.10A1-349[»]
2ZRBX-ray3.25A1-349[»]
2ZRCX-ray3.10A1-349[»]
2ZRDX-ray3.10A1-349[»]
2ZREX-ray2.90A1-349[»]
2ZRFX-ray3.00A1-349[»]
2ZRGX-ray3.50A1-349[»]
2ZRHX-ray3.20A1-349[»]
2ZRIX-ray3.30A1-349[»]
2ZRJX-ray2.60A1-349[»]
2ZRKX-ray3.20A1-349[»]
2ZRLX-ray3.70A1-349[»]
2ZRMX-ray2.80A1-349[»]
2ZRNX-ray3.30A1-349[»]
2ZROX-ray2.90A1-349[»]
2ZRPX-ray3.30A1-349[»]
ProteinModelPortaliQ59560
SMRiQ59560
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59560

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 75Phosphate binding1 Publication5

Sequence similaritiesi

Belongs to the RecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C68 Bacteria
COG0468 LUCA
HOGENOMiHOG000264120
KOiK03553
OMAiSKMGLQA
OrthoDBiPOG091H01OP

Family and domain databases

CDDicd00983 recA, 1 hit
Gene3Di3.30.250.10, 1 hit
HAMAPiMF_00268 RecA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013765 DNA_recomb/repair_RecA
IPR020584 DNA_recomb/repair_RecA_CS
IPR027417 P-loop_NTPase
IPR020588 RecA_ATP-bd
IPR023400 RecA_C
IPR020587 RecA_monomer-monomer_interface
PANTHERiPTHR22942:SF1 PTHR22942:SF1, 1 hit
PfamiView protein in Pfam
PF00154 RecA, 1 hit
PRINTSiPR00142 RECA
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54752 SSF54752, 1 hit
TIGRFAMsiTIGR02012 tigrfam_recA, 1 hit
PROSITEiView protein in PROSITE
PS00321 RECA_1, 1 hit
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q59560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQAPDREK ALELAMAQID KNFGKGSVMR LGEEVRQPIS VIPTGSISLD
60 70 80 90 100
VALGIGGLPR GRVIEIYGPE SSGKTTVALH AVANAQAAGG IAAFIDAEHA
110 120 130 140 150
LDPEYAKKLG VDTDSLLVSQ PDTGEQALEI ADMLVRSGAL DIIVIDSVAA
160 170 180 190 200
LVPRAEIEGE MGDSHVGLQA RLMSQALRKM TGALNNSGTT AIFINQLREK
210 220 230 240 250
IGVMFGSPET TTGGKALKFY ASVRLDVRRI ETLKDGTDAV GNRTRVKVVK
260 270 280 290 300
NKVSPPFKQA EFDILYGQGI SREGSLIDMG VEHGFIRKSG SWFTYEGEQL
310 320 330 340
GQGKENARKF LLENTDVANE IEKKIKEKLG IGAVVTAEAD DVLPAPVDF
Length:349
Mass (Da):37,301
Last modified:November 1, 1997 - v1
Checksum:iD85D21F5BFC5A020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99208 Genomic DNA Translation: CAA67597.1
CP000480 Genomic DNA Translation: ABK74334.1
CP001663 Genomic DNA Translation: AFP39124.1
RefSeqiWP_003894107.1, NZ_CP009494.1
YP_887057.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK74334; ABK74334; MSMEG_2723
AFP39124; AFP39124; MSMEI_2656
GeneIDi4532488
KEGGimsb:LJ00_13540
msg:MSMEI_2656
msm:MSMEG_2723
PATRICifig|246196.19.peg.2690

Similar proteinsi

Entry informationi

Entry nameiRECA_MYCS2
AccessioniPrimary (citable) accession number: Q59560
Secondary accession number(s): A0QVW9, I7G989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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