Q59548 (SECY_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein translocase subunit SecY

Gene names

Name:	secY
Ordered Locus Names:	MPN_184
ORF Names:	MP647

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently By similarity. HAMAP-Rule MF_01465
Subunit structure	Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01465.
Sequence similarities	Belongs to the SecY/SEC61-alpha family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: HAMAP protein targeting Inferred from electronic annotation. Source: HAMAP protein transport by the Sec complex Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Protein translocase subunit SecY HAMAP-Rule MF_01465		PRO_0000131736
Regions
	Transmembrane	28 – 48	21	Helical; Potential
	Transmembrane	67 – 89	23	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Transmembrane	196 – 216	21	Helical; Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Transmembrane	286 – 306	21	Helical; Potential
	Transmembrane	329 – 349	21	Helical; Potential
	Transmembrane	387 – 407	21	Helical; Potential
	Transmembrane	413 – 433	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q59548 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0301F8F0D8D4BD88	FASTA	477	51,967
	10 20 30 40 50 60 MQAKPTTAVK QKQNFGQRLF TLLRNRDFMI SFLITVVLLV LFRVLAIIPL PGIQVNQTGL 70 80 90 100 110 120 DQNSNDFFSL FNLLGGGGLN QLSLFAVGIS PYISAQIVMQ LLSTDLIPPL SKLVNSGEVG 130 140 150 160 170 180 RRKIEMITRI ITLPFALVQS FAVIQIATNS GGGSSPITLK NNGSDFVAFY IIAMTAGTYL 190 200 210 220 230 240 SVFLGDTISK KGIGNGITLL ILSGILAQLP EGFIAAYSVL SGVVVTINAT LTTAISFFIY 250 260 270 280 290 300 FMAFVTLLFA TTFITQETRK IPIQQSGQGL VTESSALPYL PIKVNSAGVI PVIFASSIMS 310 320 330 340 350 360 IPVTIAQFQP QTESRWFVED YLSLSKPTGI VLYGILVILF SFFYSYIQIN PERLAKNFEK 370 380 390 400 410 420 SGRFIPGIRP GKDTEKHIAR VLVRINFIGA PFLTVIAIIP YIVSALIHLP NSLSLGGTGI 430 440 450 460 470 IIIVTAVVEF MSALRSAATA TNYQQLRRNL AIEVQKTAQQ DKEEQLRAET PGIGNLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes." Hilbert H., Himmelreich R., Plagens H., Herrmann R. Nucleic Acids Res. 24:628-639(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34795 Genomic DNA. Translation: AAC43697.1. U00089 Genomic DNA. Translation: AAB96295.1.
PIR	S62824.
RefSeq	NP_109872.1. NC_000912.1.
3D structure databases
ProteinModelPortal	Q59548.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN184.
Proteomic databases
PaxDb	Q59548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96295; AAB96295; MPN_184.
GeneID	876763.
KEGG	mpn:MPN184.
PATRIC	20021691. VBIMycPne110_0202.
Phylogenomic databases
eggNOG	COG0201.
KO	K03076.
OMA	HIIKIVL.
ProtClustDB	PRK09204.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-191-MONOMER.
Family and domain databases
Gene3D	1.10.3370.10. 1 hit.
HAMAP	MF_01465. SecY.
InterPro	IPR026593. SecY. IPR002208. SecY/SEC61-alpha. IPR023201. SecY_su_dom. [Graphical view]
PANTHER	PTHR10906. PTHR10906. 1 hit.
Pfam	PF00344. SecY. 1 hit. [Graphical view]
PIRSF	PIRSF004557. SecY. 1 hit.
SUPFAM	SSF103491. SecY. 1 hit.
TIGRFAMs	TIGR00967. 3a0501s007. 1 hit.
PROSITE	PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECY_MYCPN

Accession

Primary (citable) accession number: Q59548

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program