Reviewed, UniProtKB/Swiss-Prot Q59544 (APHA_MORMO)

Names and origin

Protein names

Recommended name:
    Class B acid phosphatase
    EC=3.1.3.2
Alternative name(s):
    Minor phosphate-irrepressible acid phosphatase

Gene names

Name:	aphA
Synonyms:	napA

Organism

Morganella morganii (Proteus morganii)

Taxonomic identifier

582 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Morganella

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active on several organic phosphate monoesters but not on diesters, also has transphosphorylating activity from organic phosphoric acid esters to nucleosides and other compounds with free hydroxyl groups.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Enzyme regulation	Activated by magnesium, cobalt, zinc, ethanol and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and calcium.
Subunit structure	Homotetramer.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.
Biophysicochemical properties	pH dependence: Optimum pH is about 6.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro ribosome Inferred from electronic annotation. Source: InterPro
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 236	213	Class B acid phosphatase		PRO_0000024006
Sites
	Metal binding	68	1	Magnesium By similarity
	Metal binding	70	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	191	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59544-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E5F6A38D918B74D2	FASTA	236	26,683
	10 20 30 40 50 60 MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS VEQIEESLKG 70 80 90 100 110 120 QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF WEKMNNEWDK FSMPKKSGME 130 140 150 160 170 180 LVQMHLKRGD TVYFITGRSK TKTETVTKYV QEGLRIPADK MNPVIFAGDE EGQNNKVSWM 190 200 210 220 230 RDHKLKIYYG DADADIAAAR ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY

References

[1]

"Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes." Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M. Microbiology 141:147-154(1995) [PubMed: 7894706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-43, CHARACTERIZATION. Strain: RS12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78328 Genomic DNA. Translation: CAA55131.1.
3D structure databases
SMR	Q59544. Positions 28-236.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.2. 2621.
Family and domain databases
InterPro	IPR005519. Acid_phosphat_B. IPR010025. HAD-SF_ppase_IIIB_AphA. IPR003256. Ribosomal_L24. [Graphical view]
PANTHER	PTHR12903. Ribosomal_L24. 1 hit.
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_MORMO

Accession

Primary (citable) accession number: Q59544

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)