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Reviewed, UniProtKB/Swiss-Prot Q59544 (APHA_MORMO)

Last modified February 9, 2010. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Class B acid phosphatase
    EC=3.1.3.2
Alternative name(s):
    Minor phosphate-irrepressible acid phosphatase
Gene names
Name: aphA
Synonyms: napA
OrganismMorganella morganii (Proteus morganii)
Taxonomic identifier582 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeMorganella

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Active on several organic phosphate monoesters but not on diesters, also has transphosphorylating activity from organic phosphoric acid esters to nucleosides and other compounds with free hydroxyl groups.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Enzyme regulation

Activated by magnesium, cobalt, zinc, ethanol and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and calcium.

Subunit structure

Homotetramer.

Subcellular location

Periplasm Potential.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Biophysicochemical properties

pH dependence:

Optimum pH is about 6.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.1
Chain24 – 236213Class B acid phosphatase
PRO_0000024006

Sites

Metal binding681Magnesium By similarity
Metal binding701Magnesium; via carbonyl oxygen By similarity
Metal binding1911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59544-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E5F6A38D918B74D2

FASTA23626,683
        10         20         30         40         50         60 
MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS VEQIEESLKG 

        70         80         90        100        110        120 
QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF WEKMNNEWDK FSMPKKSGME 

       130        140        150        160        170        180 
LVQMHLKRGD TVYFITGRSK TKTETVTKYV QEGLRIPADK MNPVIFAGDE EGQNNKVSWM 

       190        200        210        220        230 
RDHKLKIYYG DADADIAAAR ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY 

« Hide

References

[1]"Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes."
Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M.
Microbiology 141:147-154(1995) [PubMed: 7894706] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-43, CHARACTERIZATION.
Strain: RS12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78328 Genomic DNA. Translation: CAA55131.1.

3D structure databases

SMRQ59544. Positions 28-236.
ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.3.2. 2621.

Family and domain databases

InterProIPR005519. Acid_phosphat_B.
IPR010025. HAD-SF_ppase_IIIB_AphA.
IPR003256. Ribosomal_L24.
[Graphical view]
PANTHERPTHR12903. Ribosomal_L24. 1 hit.
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_MORMO
AccessionPrimary (citable) accession number: Q59544
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents