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Protein

Class B acid phosphatase

Gene

aphA

Organism
Morganella morganii (Proteus morganii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters including 5'-AMP, 3'-AMP, pNPP, PDP, 5'-UMP, 3'-UMP, G2P, glucose 6-P and ribose 5-P. No activity toward organic phosphate diesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Activated by ethanol. Also activated by Co2+, Zn2+ and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and Ca2+. Unaffected by fluoride and tartrate.1 Publication

pH dependencei

Optimum pH is about 6 using pNPP as substrate. Retains more than 50% of maximal activity in the pH range 4-7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68NucleophileBy similarity1
Metal bindingi68MagnesiumBy similarity1
Active sitei70Proton donorBy similarity1
Metal bindingi70Magnesium; via carbonyl oxygenBy similarity1
Binding sitei176SubstrateBy similarity1
Metal bindingi191MagnesiumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.21 Publication)
Short name:
CBAP
Alternative name(s):
Minor phosphate-irrepressible acid phosphatase
Gene namesi
Name:aphA
Synonyms:napA
OrganismiMorganella morganii (Proteus morganii)
Taxonomic identifieri582 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeMorganella

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000002400624 – 236Class B acid phosphataseAdd BLAST213

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ59544
SMRiQ59544
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 137Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR005519 Acid_phosphat_B-like
IPR036412 HAD-like_sf
IPR010025 HAD-SF_ppase_IIIB_AphA
IPR023214 HAD_sf
PfamiView protein in Pfam
PF03767 Acid_phosphat_B, 1 hit
PIRSFiPIRSF017818 Acid_Ptase_B, 1 hit
SFLDiSFLDG01127 C1.3:_Acid_Phosphatase_Like, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01672 AphA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS
60 70 80 90 100
VEQIEESLKG QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF
110 120 130 140 150
WEKMNNEWDK FSMPKKSGME LVQMHLKRGD TVYFITGRSK TKTETVTKYV
160 170 180 190 200
QEGLRIPADK MNPVIFAGDE EGQNNKVSWM RDHKLKIYYG DADADIAAAR
210 220 230
ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY
Length:236
Mass (Da):26,683
Last modified:November 1, 1996 - v1
Checksum:iE5F6A38D918B74D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78328 Genomic DNA Translation: CAA55131.1

Similar proteinsi

Entry informationi

Entry nameiAPHA_MORMO
AccessioniPrimary (citable) accession number: Q59544
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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