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Q59544

- APHA_MORMO

UniProt

Q59544 - APHA_MORMO

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Morganella morganii (Proteus morganii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters including 5'-AMP, 3'-AMP, pNPP, PDP, 5'-UMP, 3'-UMP, G2P, glucose 6-P and ribose 5-P. No activity toward organic phosphate diesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Enzyme regulationi

Activated by ethanol. Also activated by Co2+, Zn2+ and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and Ca2+. Unaffected by fluoride and tartrate.1 Publication

pH dependencei

Optimum pH is about 6 using pNPP as substrate. Retains more than 50% of maximal activity in the pH range 4-7.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681NucleophileBy similarity
Metal bindingi68 – 681MagnesiumBy similarity
Active sitei70 – 701Proton donorBy similarity
Metal bindingi70 – 701Magnesium; via carbonyl oxygenBy similarity
Binding sitei176 – 1761SubstrateBy similarity
Metal bindingi191 – 1911MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2)
Short name:
CBAP
Alternative name(s):
Minor phosphate-irrepressible acid phosphatase
Gene namesi
Name:aphA
Synonyms:napA
OrganismiMorganella morganii (Proteus morganii)
Taxonomic identifieri582 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeMorganella

Subcellular locationi

Periplasm 1 Publication

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 236213Class B acid phosphatasePRO_0000024006Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ59544.
SMRiQ59544. Positions 28-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1372Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59544-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS
60 70 80 90 100
VEQIEESLKG QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF
110 120 130 140 150
WEKMNNEWDK FSMPKKSGME LVQMHLKRGD TVYFITGRSK TKTETVTKYV
160 170 180 190 200
QEGLRIPADK MNPVIFAGDE EGQNNKVSWM RDHKLKIYYG DADADIAAAR
210 220 230
ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY
Length:236
Mass (Da):26,683
Last modified:November 1, 1996 - v1
Checksum:iE5F6A38D918B74D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78328 Genomic DNA. Translation: CAA55131.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78328 Genomic DNA. Translation: CAA55131.1 .

3D structure databases

ProteinModelPortali Q59544.
SMRi Q59544. Positions 28-236.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes."
    Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M.
    Microbiology 141:147-154(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-43, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT.
    Strain: RS12.

Entry informationi

Entry nameiAPHA_MORMO
AccessioniPrimary (citable) accession number: Q59544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3