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Protein

Class B acid phosphatase

Gene

aphA

Organism
Morganella morganii (Proteus morganii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters including 5'-AMP, 3'-AMP, pNPP, PDP, 5'-UMP, 3'-UMP, G2P, glucose 6-P and ribose 5-P. No activity toward organic phosphate diesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Activated by ethanol. Also activated by Co2+, Zn2+ and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and Ca2+. Unaffected by fluoride and tartrate.1 Publication

pH dependencei

Optimum pH is about 6 using pNPP as substrate. Retains more than 50% of maximal activity in the pH range 4-7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68NucleophileBy similarity1
Metal bindingi68MagnesiumBy similarity1
Active sitei70Proton donorBy similarity1
Metal bindingi70Magnesium; via carbonyl oxygenBy similarity1
Binding sitei176SubstrateBy similarity1
Metal bindingi191MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.21 Publication)
Short name:
CBAP
Alternative name(s):
Minor phosphate-irrepressible acid phosphatase
Gene namesi
Name:aphA
Synonyms:napA
OrganismiMorganella morganii (Proteus morganii)
Taxonomic identifieri582 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeMorganella

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000002400624 – 236Class B acid phosphataseAdd BLAST213

Proteomic databases

PRIDEiQ59544.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ59544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 137Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS
60 70 80 90 100
VEQIEESLKG QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF
110 120 130 140 150
WEKMNNEWDK FSMPKKSGME LVQMHLKRGD TVYFITGRSK TKTETVTKYV
160 170 180 190 200
QEGLRIPADK MNPVIFAGDE EGQNNKVSWM RDHKLKIYYG DADADIAAAR
210 220 230
ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY
Length:236
Mass (Da):26,683
Last modified:November 1, 1996 - v1
Checksum:iE5F6A38D918B74D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78328 Genomic DNA. Translation: CAA55131.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78328 Genomic DNA. Translation: CAA55131.1.

3D structure databases

ProteinModelPortaliQ59544.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ59544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_MORMO
AccessioniPrimary (citable) accession number: Q59544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.