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Q59544

- APHA_MORMO

UniProt

Q59544 - APHA_MORMO

Protein

Class B acid phosphatase

Gene

aphA

Organism
Morganella morganii (Proteus morganii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters including 5'-AMP, 3'-AMP, pNPP, PDP, 5'-UMP, 3'-UMP, G2P, glucose 6-P and ribose 5-P. No activity toward organic phosphate diesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds.1 Publication

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Enzyme regulationi

    Activated by ethanol. Also activated by Co2+, Zn2+ and glycerol. Inhibited by EDTA, inorganic phosphate, nucleosides and Ca2+. Unaffected by fluoride and tartrate.1 Publication

    pH dependencei

    Optimum pH is about 6 using pNPP as substrate. Retains more than 50% of maximal activity in the pH range 4-7.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681NucleophileBy similarity
    Metal bindingi68 – 681MagnesiumBy similarity
    Active sitei70 – 701Proton donorBy similarity
    Metal bindingi70 – 701Magnesium; via carbonyl oxygenBy similarity
    Binding sitei176 – 1761SubstrateBy similarity
    Metal bindingi191 – 1911MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphatase (EC:3.1.3.2)
    Short name:
    CBAP
    Alternative name(s):
    Minor phosphate-irrepressible acid phosphatase
    Gene namesi
    Name:aphA
    Synonyms:napA
    OrganismiMorganella morganii (Proteus morganii)
    Taxonomic identifieri582 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeMorganella

    Subcellular locationi

    Periplasm 1 Publication

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 236213Class B acid phosphatasePRO_0000024006Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ59544.
    SMRiQ59544. Positions 28-236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 1372Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q59544-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKLTLTLSA LALALSLNSV ADAKVYMPEK VSDGVTVAQL AEQHAIHWIS    50
    VEQIEESLKG QPMAVGFDID DTVLFSSPGF YRGKLEYSPN DYSYLKNPEF 100
    WEKMNNEWDK FSMPKKSGME LVQMHLKRGD TVYFITGRSK TKTETVTKYV 150
    QEGLRIPADK MNPVIFAGDE EGQNNKVSWM RDHKLKIYYG DADADIAAAR 200
    ELNIRGIRVL RASNSSYQPL PKAGQFGEEV VINSEY 236
    Length:236
    Mass (Da):26,683
    Last modified:November 1, 1996 - v1
    Checksum:iE5F6A38D918B74D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78328 Genomic DNA. Translation: CAA55131.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78328 Genomic DNA. Translation: CAA55131.1 .

    3D structure databases

    ProteinModelPortali Q59544.
    SMRi Q59544. Positions 28-236.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes."
      Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M.
      Microbiology 141:147-154(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-43, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT.
      Strain: RS12.

    Entry informationi

    Entry nameiAPHA_MORMO
    AccessioniPrimary (citable) accession number: Q59544
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3