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Reviewed, UniProtKB/Swiss-Prot Q59543 (DHML_METME)

Last modified November 25, 2008. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methylamine dehydrogenase light chain
      Short name=MADH
    EC=1.4.99.3
Gene names
Name: mauA
OrganismMethylophilus methylotrophus (Bacterium W3A1)
Taxonomic identifier17 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylophilus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.

Catalytic activity

RCH(2)NH(2) + H(2)O + acceptor = RCHO + NH(3) + reduced acceptor.

Cofactor

Contains 1 tryptophan tryptophylquinone per subunit By similarity.

Pathway

One-carbon metabolism; methylamine degradation; formaldehyde from methylamine: step 1/1.

Subunit structure

Heterotetramer of two light and two heavy chains.

Subcellular location

Periplasm.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Tryptophan tryptophylquinone (TTQ) is formed by oxidation of the indole ring of a tryptophan to form tryptophylquinone followed by covalent cross-linking with another tryptophan residue.

Sequence similarities

Belongs to the aromatic amine dehydrogenase light chain family.

Ontologies

Keywords

   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionOxidoreductase
   PTMTTQ

Gene Ontology (GO)

   Biological processcellular amine metabolic process

Inferred from electronic annotation. Source: InterPro

electron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular functionamine dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5757Tat-type signal Potential
Chain58 – 187130Methylamine dehydrogenase light chain
PRO_0000025574

Amino acid modifications

Modified residue1131Tryptophylquinone By similarity
Disulfide bond79 ↔ 144 By similarity
Disulfide bond85 ↔ 117 By similarity
Disulfide bond92 ↔ 177 By similarity
Disulfide bond94 ↔ 142 By similarity
Disulfide bond102 ↔ 133 By similarity
Disulfide bond134 ↔ 165 By similarity
Cross-link113 ↔ 164Tryptophan tryptophylquinone (Trp-Trp) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59543-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E33FEBAE30CC5CED

FASTA18720,237
        10         20         30         40         50         60 
MKKDTGFDSK IEKLARTTAS KTGRRGFIGR LGGFLVGSAL LPLLPVDRRS RLGGEVQAAT 

        70         80         90        100        110        120 
TGNLTRSGFK PQDKDPKACE YWRHCTIDGN LCDCCGGTLT SCPPGSSLSP SSWVASCYNP 

       130        140        150        160        170        180 
GDQQTYLIAY RDCCGKQTCG RCNCVNTQGE LPVYRPEFNN DIVWCFGADN DAMTYHCTIS 


PIVGKAS 

« Hide

References

[1]"Organization of the methylamine utilization (mau) genes in Methylophilus methylotrophus W3A1-NS."
Chistoserdov A.Y., McIntire W.S., Mathews F.S., Lidstrom M.E.
J. Bacteriol. 176:4073-4080(1994) [PubMed: 8021188] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

L26407 Genomic DNA. Translation: AAB46951.1.
PIRT10073.

3D structure databases

HSSPHSSP built from PDB template 2BBK based on UniProtKB P22619.
SMRQ59543. Positions 66-187.
ModBaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MON-3908.

Family and domain databases

InterProIPR016008. Amine_DHase_bsu.
IPR004229. MeN_DHase_Ltc.
IPR013504. MeN_DHase_Ltc_C.
[Graphical view]
Gene3DG3DSA:2.60.30.10. MADH_Lt_C. 1 hit.
PfamPF02975. Me-amine-dh_L. 1 hit.
[Graphical view]
PIRSFPIRSF000192. Amine_dh_beta. 1 hit.
TIGRFAMsTIGR02659. TTQ_MADH_Lt. 1 hit.
PROSITEPS51318. TAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHML_METME
AccessionPrimary (citable) accession number: Q59543
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents