Reviewed, UniProtKB/Swiss-Prot Q59543 (DHML_METME)

Names and origin

Protein names

Recommended name:
    Methylamine dehydrogenase light chain
      Short name=MADH
    EC=1.4.99.3

Gene names

Name:

mauA

Organism

Methylophilus methylotrophus (Bacterium W3A1)

Taxonomic identifier

17 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylophilus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
Catalytic activity	RCH(2)NH(2) + H(2)O + acceptor = RCHO + NH(3) + reduced acceptor.
Cofactor	Contains 1 tryptophan tryptophylquinone per subunit By similarity.
Pathway	One-carbon metabolism; methylamine degradation; formaldehyde from methylamine: step 1/1.
Subunit structure	Heterotetramer of two light and two heavy chains.
Subcellular location	Periplasm.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Tryptophan tryptophylquinone (TTQ) is formed by oxidation of the indole ring of a tryptophan to form tryptophylquinone followed by covalent cross-linking with another tryptophan residue.
Sequence similarities	Belongs to the aromatic amine dehydrogenase light chain family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Oxidoreductase
PTM	TTQ
Gene Ontology (GO)
Biological process	cellular amine metabolic process Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	amine dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 57	57	Tat-type signal Potential
	Chain	58 – 187	130	Methylamine dehydrogenase light chain		PRO_0000025574
Amino acid modifications
	Modified residue	113	1	Tryptophylquinone By similarity
	Disulfide bond	79 ↔ 144		By similarity
	Disulfide bond	85 ↔ 117		By similarity
	Disulfide bond	92 ↔ 177		By similarity
	Disulfide bond	94 ↔ 142		By similarity
	Disulfide bond	102 ↔ 133		By similarity
	Disulfide bond	134 ↔ 165		By similarity
	Cross-link	113 ↔ 164		Tryptophan tryptophylquinone (Trp-Trp) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59543-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E33FEBAE30CC5CED	FASTA	187	20,237
	10 20 30 40 50 60 MKKDTGFDSK IEKLARTTAS KTGRRGFIGR LGGFLVGSAL LPLLPVDRRS RLGGEVQAAT 70 80 90 100 110 120 TGNLTRSGFK PQDKDPKACE YWRHCTIDGN LCDCCGGTLT SCPPGSSLSP SSWVASCYNP 130 140 150 160 170 180 GDQQTYLIAY RDCCGKQTCG RCNCVNTQGE LPVYRPEFNN DIVWCFGADN DAMTYHCTIS PIVGKAS

References

[1]	"Organization of the methylamine utilization (mau) genes in Methylophilus methylotrophus W3A1-NS." Chistoserdov A.Y., McIntire W.S., Mathews F.S., Lidstrom M.E. J. Bacteriol. 176:4073-4080(1994) [PubMed: 8021188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26407 Genomic DNA. Translation: AAB46951.1.
PIR	T10073.
3D structure databases
HSSP	HSSP built from PDB template 2BBK based on UniProtKB P22619.
SMR	Q59543. Positions 66-187.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-3908.
Family and domain databases
InterPro	IPR016008. Amine_DHase_bsu. IPR004229. MeN_DHase_Ltc. IPR013504. MeN_DHase_Ltc_C. [Graphical view]
Gene3D	G3DSA:2.60.30.10. MADH_Lt_C. 1 hit.
Pfam	PF02975. Me-amine-dh_L. 1 hit. [Graphical view]
PIRSF	PIRSF000192. Amine_dh_beta. 1 hit.
TIGRFAMs	TIGR02659. TTQ_MADH_Lt. 1 hit.
PROSITE	PS51318. TAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHML_METME

Accession

Primary (citable) accession number: Q59543

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)