Q59542 (DHMH_METME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylamine dehydrogenase heavy chain
Short name=MADH
EC=1.4.9.1

Alternative name(s):
Methylamine dehydrogenase (amicyanin)

Gene names

Name:

mauB

Organism

Methylophilus methylotrophus (Bacterium W3A1)

Taxonomic identifier

17 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylophilus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
Catalytic activity	Methylamine + H2O + amicyanin = formaldehyde + ammonia + reduced amicyanin.
Subunit structure	Tetramer of two light and two heavy chains.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the aromatic amine dehydrogenase heavy chain family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW methylamine metabolic process Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – 405	369	Methylamine dehydrogenase heavy chain		PRO_0000025579

Sequences

	Sequence		Length	Mass (Da)
	Q59542 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D7B0FB8842749E94	FASTA	405	45,244
	10 20 30 40 50 60 MTTFDHPSMI RQPKPTGLAG GLVLAALMLS SSLALADATP NQLGSEITAK LQDETSIAIA 70 80 90 100 110 120 PASDSKRVYV LDPGNFHMTS TVYTIDGKSS KLLGMTDAGK LPNVMVASDG KFLAIANTMY 130 140 150 160 170 180 SRVARGKRDD YLELIDTKTH QPIADIDIPE GRFLTGVFER TAGLSVDDKH LLFQQFSPSP 190 200 210 220 230 240 GVGLVDLQQK AFVKIMNVPD CYHIFPTANQ NFFMHCRDGS LMQFTYDSKG NTKQKPTKIF 250 260 270 280 290 300 HAEKEYLLNN PYYSNSNNHL TWPTYEGKIF QAKLSESGAE FLKPIEVFTD KEKADKWRPG 310 320 330 340 350 360 GWQTIAFHKA RNELYLLADQ REKWTHKLPS RFVFVVDATS GKRLRRIELK HEINSIAVSQ 370 380 390 400 DDKPYLYAVS EEAKTLFTFD AVNGKALSSI DELGRAPSMI FIADK

References

[1]	"Organization of the methylamine utilization (mau) genes in Methylophilus methylotrophus W3A1-NS." Chistoserdov A.Y., McIntire W.S., Mathews F.S., Lidstrom M.E. J. Bacteriol. 176:4073-4080(1994) [PubMed: 8021188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26407 Genomic DNA. Translation: AAB46948.1.
PIR	T10070.
3D structure databases
ProteinModelPortal	Q59542.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-3907.
Family and domain databases
InterPro	IPR013476. MeN_DH_hvc. IPR009451. Metamine_DH_Hsu. IPR011044. Quino_amine_DH_bsu. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF06433. Me-amine-dh_H. 1 hit. [Graphical view]
PIRSF	PIRSF017797. TTQ_MADH_Hv. 1 hit.
SUPFAM	SSF50969. Amine_DH_B_like. 1 hit.
TIGRFAMs	TIGR02658. TTQ_MADH_Hv. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHMH_METME

Accession

Primary (citable) accession number: Q59542

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program