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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei5ATPUniRule annotation1
Binding sitei45ATPUniRule annotation1
Binding sitei72ATPUniRule annotation1
Binding sitei338ATPUniRule annotation1
Metal bindingi426Magnesium 1; catalyticUniRule annotation1
Metal bindingi499Magnesium 1; catalyticUniRule annotation1
Metal bindingi499Magnesium 2UniRule annotation1
Metal bindingi501Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 119ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGAL710127:GC09-475-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:MYCGA4200
ORF Names:MGA_0060
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454311 – 633DNA topoisomerase 4 subunit BAdd BLAST633

Proteomic databases

PRIDEiQ59526.

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei451Interaction with DNAUniRule annotation1
Sitei454Interaction with DNAUniRule annotation1
Sitei506Interaction with DNAUniRule annotation1
Sitei619Interaction with DNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliQ59526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini420 – 534ToprimUniRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 2 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000075155.
KOiK02622.
OMAiPVGMHKL.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSNYNEKHI KVLKGLEPVR KRPGMYIGST DTRGLHHLVW ELFDNAVDEA
60 70 80 90 100
LNGSANKIEV VHKKDGSIIV TDNGRGIPVG KNLATNLSTV DTVYTVLHAG
110 120 130 140 150
GKFDDQAYKV SGGLHGVGAS VVNALSRKLI VTVHRDGGMY ESIYQDGGKI
160 170 180 190 200
IQPLKKVGNS TKHGTIVQFW PDPTIFKNIQ FNPYMIKERL HESSFLFKGL
210 220 230 240 250
KIVFVDENNP ELSQTFIAKD GILEYLKYVN ENKKSISKII PFKGDYEKIV
260 270 280 290 300
VDGCFQYTDN ESELIISFAN SVKTSEGGSH ENGFKQAMLE TINDYSKKYK
310 320 330 340 350
LINNKDKGFD WSDLKEGLSV VLSVQVPEKI IAYEGQTKNK LFTQEVKVAV
360 370 380 390 400
AKILTQQLFY FLEENQADAK QLIERFKLIK EAKEAAKKAK ENTKKLKSAK
410 420 430 440 450
SERVLYGKLT PAQQKNPLQN EIFLVEGDSA GGTAKSGRDK RFQAILPLRG
460 470 480 490 500
KVVNVEKSRL QDLLKNEEIL SIISCLGCGI GNNFNIKNLK YHKIIIMTDA
510 520 530 540 550
DTDGAHIQVL LLTFFYRYMK ELIEQGKVYI ALAPLYKISA KNSNKSAFAW
560 570 580 590 600
DDYQLDELRT KYGSYEVQRY KGLGEMNSEQ LWTTTMDPSK RQLIQVSIQN
610 620 630
AAQAERKVSI LMGDDASIRK NWINENVDFS IEE
Length:633
Mass (Da):71,373
Last modified:August 15, 2003 - v2
Checksum:i6DF59F0FBC1DA155
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157V → M in AAA91333 (PubMed:7783737).Curated1
Sequence conflicti159N → S in AAA91333 (PubMed:7783737).Curated1
Sequence conflicti167V → I in AAA91333 (PubMed:7783737).Curated1
Sequence conflicti235S → T in AAA91333 (PubMed:7783737).Curated1
Sequence conflicti539S → T in AAA91333 (PubMed:7783737).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35044 Genomic DNA. Translation: AAA91333.1.
AE015450 Genomic DNA. Translation: AAP56770.2.
RefSeqiWP_011113666.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56770; AAP56770; MGA_0060.
GeneIDi1089870.
KEGGimga:MGA_0060.
PATRICi20008669. VBIMycGal115357_0470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35044 Genomic DNA. Translation: AAA91333.1.
AE015450 Genomic DNA. Translation: AAP56770.2.
RefSeqiWP_011113666.1. NC_004829.2.

3D structure databases

ProteinModelPortaliQ59526.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ59526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56770; AAP56770; MGA_0060.
GeneIDi1089870.
KEGGimga:MGA_0060.
PATRICi20008669. VBIMycGal115357_0470.

Phylogenomic databases

HOGENOMiHOG000075155.
KOiK02622.
OMAiPVGMHKL.

Enzyme and pathway databases

BioCyciMGAL710127:GC09-475-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPARE_MYCGA
AccessioniPrimary (citable) accession number: Q59526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: August 15, 2003
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.