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Protein

Mycinamicin IV hydroxylase/epoxidase

Gene

mycG

Organism
Micromonospora griseorubida
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic. Catalyzes consecutive hydroxylation (at C14) and epoxidation (at C12-C13) reactions with mycinamicin IV as initial substrate, leading to mycinamicin II. These reactions require prior dimethylation of 6-deoxyallose to mycinose for effective conversion by the dual function MycG enzyme.3 Publications

Cofactori

heme1 Publication

Kineticsi

kcat is 415.7 min(-1) for the epoxydation of mycinamicin V.1 Publication

Manual assertion based on experiment ini

  1. KM=16.2 µM for mycinamicin V1 Publication

    Pathwayi: mycinamicin biosynthesis

    This protein is involved in the pathway mycinamicin biosynthesis, which is part of Antibiotic biosynthesis.1 Publication1 Publication
    View all proteins of this organism that are known to be involved in the pathway mycinamicin biosynthesis and in Antibiotic biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei81Substrate; via carbonyl oxygenCombined sources1 Publication1
    Binding sitei91Heme1 Publication1
    Binding sitei95Heme1 Publication1
    Binding sitei288Heme1 Publication1
    Binding sitei344Heme1 Publication1
    Metal bindingi346Iron (heme axial ligand)Combined sources1 Publication1

    GO - Molecular functioni

    • heme binding Source: UniProtKB
    • iron ion binding Source: UniProtKB
    • monooxygenase activity Source: UniProtKB
    • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

    GO - Biological processi

    • antibiotic biosynthetic process Source: UniProtKB
    • oxidation-reduction process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18369.
    UniPathwayiUPA01019.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mycinamicin IV hydroxylase/epoxidase1 Publication (EC:1.14.-.-1 Publication)
    Alternative name(s):
    Cytochrome P450 MycG1 Publication
    Multifunctional P450 enzyme1 Publication
    Mycinamicin biosynthesis protein G1 Publication
    Gene namesi
    Name:mycG1 Publication
    OrganismiMicromonospora griseorubida
    Taxonomic identifieri28040 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeMicromonospora

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene do not produce mycinamicin I, mycinamicin II and mycinamicin V, and accumulate mycinamicin IV.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi286F → A or V: Decrease in catalytic activiy. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004347651 – 397Mycinamicin IV hydroxylase/epoxidaseAdd BLAST397

    Structurei

    Secondary structure

    1397
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi6 – 9Combined sources4
    Beta strandi14 – 16Combined sources3
    Helixi21 – 29Combined sources9
    Beta strandi31 – 36Combined sources6
    Beta strandi38 – 40Combined sources3
    Beta strandi43 – 46Combined sources4
    Helixi49 – 56Combined sources8
    Beta strandi61 – 63Combined sources3
    Helixi64 – 67Combined sources4
    Beta strandi73 – 77Combined sources5
    Helixi83 – 85Combined sources3
    Helixi90 – 101Combined sources12
    Helixi104 – 109Combined sources6
    Helixi111 – 128Combined sources18
    Beta strandi130 – 133Combined sources4
    Helixi134 – 137Combined sources4
    Turni138 – 141Combined sources4
    Helixi142 – 151Combined sources10
    Helixi155 – 157Combined sources3
    Helixi158 – 166Combined sources9
    Helixi176 – 199Combined sources24
    Helixi205 – 211Combined sources7
    Helixi221 – 252Combined sources32
    Helixi254 – 262Combined sources9
    Helixi264 – 266Combined sources3
    Helixi267 – 277Combined sources11
    Beta strandi281 – 283Combined sources3
    Beta strandi288 – 292Combined sources5
    Beta strandi294 – 296Combined sources3
    Beta strandi299 – 301Combined sources3
    Beta strandi306 – 309Combined sources4
    Helixi311 – 314Combined sources4
    Turni318 – 320Combined sources3
    Turni322 – 325Combined sources4
    Helixi342 – 344Combined sources3
    Helixi349 – 366Combined sources18
    Beta strandi371 – 374Combined sources4
    Helixi376 – 378Combined sources3
    Beta strandi385 – 387Combined sources3
    Beta strandi394 – 396Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2Y46X-ray1.83A/B/C1-397[»]
    2Y5NX-ray1.62A/B1-397[»]
    2Y5ZX-ray2.06A/B/C1-397[»]
    2Y98X-ray1.65A1-397[»]
    2YCAX-ray1.80A1-397[»]
    2YGXX-ray2.39A/B/C/D1-397[»]
    3ZSNX-ray1.90A/B/C1-397[»]
    4AW3X-ray2.05A/B1-397[»]
    ProteinModelPortaliQ59523.
    SMRiQ59523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002397. Cyt_P450_B.
    IPR017972. Cyt_P450_CS.
    [Graphical view]
    PfamiPF00067. p450. 2 hits.
    [Graphical view]
    PRINTSiPR00359. BP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59523-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTSAEPRAYP FNDVHGLTLA GRYGELQETE PVSRVRPPYG EEAWLVTRYE
    60 70 80 90 100
    DVRAVLGDGR FVRGPSMTRD EPRTRPEMVK GGLLSMDPPE HSRLRRLVVK
    110 120 130 140 150
    AFTARRAESL RPRAREIAHE LVDQMAATGQ PADLVAMFAR QLPVRVICEL
    160 170 180 190 200
    LGVPSADHDR FTRWSGAFLS TAEVTAEEMQ EAAEQAYAYM GDLIDRRRKE
    210 220 230 240 250
    PTDDLVSALV QARDQQDSLS EQELLDLAIG LLVAGYESTT TQIADFVYLL
    260 270 280 290 300
    MTRPELRRQL LDRPELIPSA VEELTRWVPL GVGTAFPRYA VEDVTLRGVT
    310 320 330 340 350
    IRAGEPVLAS TGAANRDQAQ FPDADRIDVD RTPNQHLGFG HGVHHCLGAP
    360 370 380 390
    LARVELQVAL EVLLQRLPGI RLGIPETQLR WSEGMLLRGP LELPVVW
    Length:397
    Mass (Da):44,332
    Last modified:November 1, 1996 - v1
    Checksum:i0FE6A251BEB5E233
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D16098 Genomic DNA. Translation: BAA03672.1.
    PIRiS51594.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D16098 Genomic DNA. Translation: BAA03672.1.
    PIRiS51594.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2Y46X-ray1.83A/B/C1-397[»]
    2Y5NX-ray1.62A/B1-397[»]
    2Y5ZX-ray2.06A/B/C1-397[»]
    2Y98X-ray1.65A1-397[»]
    2YCAX-ray1.80A1-397[»]
    2YGXX-ray2.39A/B/C/D1-397[»]
    3ZSNX-ray1.90A/B/C1-397[»]
    4AW3X-ray2.05A/B1-397[»]
    ProteinModelPortaliQ59523.
    SMRiQ59523.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA01019.
    BioCyciMetaCyc:MONOMER-18369.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002397. Cyt_P450_B.
    IPR017972. Cyt_P450_CS.
    [Graphical view]
    PfamiPF00067. p450. 2 hits.
    [Graphical view]
    PRINTSiPR00359. BP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMYCG_MICGR
    AccessioniPrimary (citable) accession number: Q59523
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 11, 2015
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.