Reviewed, UniProtKB/Swiss-Prot Q59517 (BLAF_MYCFO)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

blaF

Organism

Mycobacterium fortuitum

Taxonomic identifier

1766 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Tat-type signal

Chain

33 – 294

262

Beta-lactamase

PRO_0000017003

Regions

Region

237 – 239

3

Substrate binding By similarity

Sites

Active site

75

1

Acyl-ester intermediate

Natural variations

Natural variant

82

1

A → S in amoxcillin-resistant strain.

Natural variant

116

1

A → E in amoxcillin-resistant strain.

Secondary structure

1

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294

Helix Strand Turn

Details...

Helix

34 – 46

13

Beta strand

48 – 56

9

Turn

57 – 59

3

Beta strand

62 – 66

5

Helix

74 – 76

3

Helix

77 – 90

14

Beta strand

99 – 101

3

Helix

104 – 106

3

Helix

112 – 115

4

Turn

116 – 119

4

Beta strand

120 – 123

4

Helix

124 – 133

10

Helix

137 – 147

11

Helix

149 – 159

11

Helix

173 – 176

4

Helix

188 – 200

13

Beta strand

201 – 204

4

Helix

206 – 217

12

Helix

224 – 227

4

Beta strand

233 – 240

8

Beta strand

246 – 253

8

Beta strand

259 – 271

13

Helix

279 – 293

15

Sequences

	Sequence		Length	Mass (Da)
	Q59517-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 87DE5D6333867B24	FASTA	294	30,951
	10 20 30 40 50 60 MTGLSRRNVL IGSLVAAAAV GAGVGGAAPA FAAPIDDQLA ELERRDNVLI GLYAANLQSG 70 80 90 100 110 120 RRITHRLDEM FAMCSTFKGY AAARVLQMAE HGEISLDNRV FVDADALVPN SPVTEARAGA 130 140 150 160 170 180 EMTLAELCQA ALQRSDNTAA NLLLKTIGGP AAVTAFARSV GDERTRLDRW EVELNSAIPG 190 200 210 220 230 240 DPRDTSTAAA LAVGYRAILA GDALSPPQRG LLEDWMRANQ TSSMRAGLPE GWTTADKTGS 250 260 270 280 290 GDYGSTNDAG IAFGPDGQRL LLVMMTRSQA HDPKAENLRP LIGELTALVL PSLL

References

[1]	"Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer." Timm J.C.S., Perilli M.G., Duez C., Trias J., Orefici G., Fattorini L., Amicosante G., Oratore A., Joris B. Mol. Microbiol. 12:491-504(1994) [PubMed: 8065266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The crystal structure of the class A beta-lactamase from Mycobacterium fortuitum: structural basis for a broad substrate specificity." Sauvage E., Fonze E., Vermeire M., Galleni M., Quinting B., Frere J.-M., Charlier P. Submitted (SEP-1998) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). Strain: D316.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L25634 Unassigned DNA. Translation: AAA19882.1.

PIR

S49543.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CC1	X-ray	2.13	A	33-294	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.5.2.6. 141585.

Family and domain databases

InterPro

IPR001466. Beta_lactamase-related.
IPR000871. Beta_lactamase_A/D.
IPR006311. TAT_signal.
IPR017909. Twin_arg_translocation_Tat.
[Graphical view]

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

TIGRFAMs

TIGR01409. TAT_signal_seq. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAF_MYCFO

Accession

Primary (citable) accession number: Q59517

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)