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Protein

Beta-lactamase

Gene

blaF

Organism
Mycobacterium fortuitum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Acyl-ester intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 3498.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:blaF
OrganismiMycobacterium fortuitum
Taxonomic identifieri1766 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalAdd BLAST32
ChainiPRO_000001700333 – 294Beta-lactamaseAdd BLAST262

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 46Combined sources13
Beta strandi48 – 56Combined sources9
Turni57 – 59Combined sources3
Beta strandi62 – 66Combined sources5
Helixi74 – 76Combined sources3
Helixi77 – 90Combined sources14
Beta strandi99 – 101Combined sources3
Helixi104 – 106Combined sources3
Helixi112 – 115Combined sources4
Turni116 – 119Combined sources4
Beta strandi120 – 123Combined sources4
Helixi124 – 133Combined sources10
Helixi137 – 147Combined sources11
Helixi149 – 159Combined sources11
Helixi173 – 176Combined sources4
Helixi188 – 200Combined sources13
Beta strandi201 – 204Combined sources4
Helixi206 – 217Combined sources12
Helixi224 – 227Combined sources4
Beta strandi233 – 240Combined sources8
Beta strandi246 – 253Combined sources8
Beta strandi259 – 271Combined sources13
Helixi279 – 293Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CC1X-ray2.13A33-294[»]
ProteinModelPortaliQ59517.
SMRiQ59517.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59517.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni237 – 239Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
IPR006311. TAT_signal.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGLSRRNVL IGSLVAAAAV GAGVGGAAPA FAAPIDDQLA ELERRDNVLI
60 70 80 90 100
GLYAANLQSG RRITHRLDEM FAMCSTFKGY AAARVLQMAE HGEISLDNRV
110 120 130 140 150
FVDADALVPN SPVTEARAGA EMTLAELCQA ALQRSDNTAA NLLLKTIGGP
160 170 180 190 200
AAVTAFARSV GDERTRLDRW EVELNSAIPG DPRDTSTAAA LAVGYRAILA
210 220 230 240 250
GDALSPPQRG LLEDWMRANQ TSSMRAGLPE GWTTADKTGS GDYGSTNDAG
260 270 280 290
IAFGPDGQRL LLVMMTRSQA HDPKAENLRP LIGELTALVL PSLL
Length:294
Mass (Da):30,951
Last modified:November 1, 1996 - v1
Checksum:i87DE5D6333867B24
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti82A → S in amoxcillin-resistant strain. 1
Natural varianti116A → E in amoxcillin-resistant strain. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25634 Unassigned DNA. Translation: AAA19882.1.
PIRiS49543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25634 Unassigned DNA. Translation: AAA19882.1.
PIRiS49543.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CC1X-ray2.13A33-294[»]
ProteinModelPortaliQ59517.
SMRiQ59517.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.5.2.6. 3498.

Miscellaneous databases

EvolutionaryTraceiQ59517.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
IPR006311. TAT_signal.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAF_MYCFO
AccessioniPrimary (citable) accession number: Q59517
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.