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Q59517

- BLAF_MYCFO

UniProt

Q59517 - BLAF_MYCFO

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Protein

Beta-lactamase

Gene

blaF

Organism
Mycobacterium fortuitum
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:blaF
OrganismiMycobacterium fortuitum
Taxonomic identifieri1766 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalAdd
BLAST
Chaini33 – 294262Beta-lactamasePRO_0000017003Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Structurei

Secondary structure

1
294
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 4613
Beta strandi48 – 569
Turni57 – 593
Beta strandi62 – 665
Helixi74 – 763
Helixi77 – 9014
Beta strandi99 – 1013
Helixi104 – 1063
Helixi112 – 1154
Turni116 – 1194
Beta strandi120 – 1234
Helixi124 – 13310
Helixi137 – 14711
Helixi149 – 15911
Helixi173 – 1764
Helixi188 – 20013
Beta strandi201 – 2044
Helixi206 – 21712
Helixi224 – 2274
Beta strandi233 – 2408
Beta strandi246 – 2538
Beta strandi259 – 27113
Helixi279 – 29315

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CC1X-ray2.13A33-294[»]
ProteinModelPortaliQ59517.
SMRiQ59517. Positions 33-294.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59517.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
IPR006311. TAT_signal.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59517-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGLSRRNVL IGSLVAAAAV GAGVGGAAPA FAAPIDDQLA ELERRDNVLI
60 70 80 90 100
GLYAANLQSG RRITHRLDEM FAMCSTFKGY AAARVLQMAE HGEISLDNRV
110 120 130 140 150
FVDADALVPN SPVTEARAGA EMTLAELCQA ALQRSDNTAA NLLLKTIGGP
160 170 180 190 200
AAVTAFARSV GDERTRLDRW EVELNSAIPG DPRDTSTAAA LAVGYRAILA
210 220 230 240 250
GDALSPPQRG LLEDWMRANQ TSSMRAGLPE GWTTADKTGS GDYGSTNDAG
260 270 280 290
IAFGPDGQRL LLVMMTRSQA HDPKAENLRP LIGELTALVL PSLL
Length:294
Mass (Da):30,951
Last modified:November 1, 1996 - v1
Checksum:i87DE5D6333867B24
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti82 – 821A → S in amoxcillin-resistant strain.
Natural varianti116 – 1161A → E in amoxcillin-resistant strain.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25634 Unassigned DNA. Translation: AAA19882.1.
PIRiS49543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25634 Unassigned DNA. Translation: AAA19882.1 .
PIRi S49543.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CC1 X-ray 2.13 A 33-294 [» ]
ProteinModelPortali Q59517.
SMRi Q59517. Positions 33-294.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q59517.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
IPR006311. TAT_signal.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer."
    Timm J.C.S., Perilli M.G., Duez C., Trias J., Orefici G., Fattorini L., Amicosante G., Oratore A., Joris B.
    Mol. Microbiol. 12:491-504(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The crystal structure of the class A beta-lactamase from Mycobacterium fortuitum: structural basis for a broad substrate specificity."
    Sauvage E., Fonze E., Vermeire M., Galleni M., Quinting B., Frere J.-M., Charlier P.
    Submitted (SEP-1998) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    Strain: D316.

Entry informationi

Entry nameiBLAF_MYCFO
AccessioniPrimary (citable) accession number: Q59517
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3