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Q59517 (BLAF_MYCFO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:blaF
OrganismMycobacterium fortuitum
Taxonomic identifier1766 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal
Chain33 – 294262Beta-lactamase
PRO_0000017003

Regions

Region237 – 2393Substrate binding By similarity

Sites

Active site751Acyl-ester intermediate

Natural variations

Natural variant821A → S in amoxcillin-resistant strain.
Natural variant1161A → E in amoxcillin-resistant strain.

Secondary structure

......................................... 294
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q59517 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 87DE5D6333867B24

FASTA29430,951
        10         20         30         40         50         60 
MTGLSRRNVL IGSLVAAAAV GAGVGGAAPA FAAPIDDQLA ELERRDNVLI GLYAANLQSG 

        70         80         90        100        110        120 
RRITHRLDEM FAMCSTFKGY AAARVLQMAE HGEISLDNRV FVDADALVPN SPVTEARAGA 

       130        140        150        160        170        180 
EMTLAELCQA ALQRSDNTAA NLLLKTIGGP AAVTAFARSV GDERTRLDRW EVELNSAIPG 

       190        200        210        220        230        240 
DPRDTSTAAA LAVGYRAILA GDALSPPQRG LLEDWMRANQ TSSMRAGLPE GWTTADKTGS 

       250        260        270        280        290 
GDYGSTNDAG IAFGPDGQRL LLVMMTRSQA HDPKAENLRP LIGELTALVL PSLL 

« Hide

References

[1]"Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer."
Timm J.C.S., Perilli M.G., Duez C., Trias J., Orefici G., Fattorini L., Amicosante G., Oratore A., Joris B.
Mol. Microbiol. 12:491-504(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"The crystal structure of the class A beta-lactamase from Mycobacterium fortuitum: structural basis for a broad substrate specificity."
Sauvage E., Fonze E., Vermeire M., Galleni M., Quinting B., Frere J.-M., Charlier P.
Submitted (SEP-1998) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Strain: D316.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25634 Unassigned DNA. Translation: AAA19882.1.
PIRS49543.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CC1X-ray2.13A33-294[»]
ProteinModelPortalQ59517.
SMRQ59517. Positions 33-294.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
IPR006311. TAT_signal.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ59517.

Entry information

Entry nameBLAF_MYCFO
AccessionPrimary (citable) accession number: Q59517
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 13, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references