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Q59517

- BLAF_MYCFO

UniProt

Q59517 - BLAF_MYCFO

Protein

Beta-lactamase

Gene

blaF

Organism
Mycobacterium fortuitum
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751Acyl-ester intermediate

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:blaF
    OrganismiMycobacterium fortuitum
    Taxonomic identifieri1766 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalAdd
    BLAST
    Chaini33 – 294262Beta-lactamasePRO_0000017003Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Structurei

    Secondary structure

    1
    294
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi34 – 4613
    Beta strandi48 – 569
    Turni57 – 593
    Beta strandi62 – 665
    Helixi74 – 763
    Helixi77 – 9014
    Beta strandi99 – 1013
    Helixi104 – 1063
    Helixi112 – 1154
    Turni116 – 1194
    Beta strandi120 – 1234
    Helixi124 – 13310
    Helixi137 – 14711
    Helixi149 – 15911
    Helixi173 – 1764
    Helixi188 – 20013
    Beta strandi201 – 2044
    Helixi206 – 21712
    Helixi224 – 2274
    Beta strandi233 – 2408
    Beta strandi246 – 2538
    Beta strandi259 – 27113
    Helixi279 – 29315

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CC1X-ray2.13A33-294[»]
    ProteinModelPortaliQ59517.
    SMRiQ59517. Positions 33-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ59517.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 2393Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    IPR006311. TAT_signal.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q59517-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGLSRRNVL IGSLVAAAAV GAGVGGAAPA FAAPIDDQLA ELERRDNVLI    50
    GLYAANLQSG RRITHRLDEM FAMCSTFKGY AAARVLQMAE HGEISLDNRV 100
    FVDADALVPN SPVTEARAGA EMTLAELCQA ALQRSDNTAA NLLLKTIGGP 150
    AAVTAFARSV GDERTRLDRW EVELNSAIPG DPRDTSTAAA LAVGYRAILA 200
    GDALSPPQRG LLEDWMRANQ TSSMRAGLPE GWTTADKTGS GDYGSTNDAG 250
    IAFGPDGQRL LLVMMTRSQA HDPKAENLRP LIGELTALVL PSLL 294
    Length:294
    Mass (Da):30,951
    Last modified:November 1, 1996 - v1
    Checksum:i87DE5D6333867B24
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti82 – 821A → S in amoxcillin-resistant strain.
    Natural varianti116 – 1161A → E in amoxcillin-resistant strain.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25634 Unassigned DNA. Translation: AAA19882.1.
    PIRiS49543.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25634 Unassigned DNA. Translation: AAA19882.1 .
    PIRi S49543.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CC1 X-ray 2.13 A 33-294 [» ]
    ProteinModelPortali Q59517.
    SMRi Q59517. Positions 33-294.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q59517.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    IPR006311. TAT_signal.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer."
      Timm J.C.S., Perilli M.G., Duez C., Trias J., Orefici G., Fattorini L., Amicosante G., Oratore A., Joris B.
      Mol. Microbiol. 12:491-504(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "The crystal structure of the class A beta-lactamase from Mycobacterium fortuitum: structural basis for a broad substrate specificity."
      Sauvage E., Fonze E., Vermeire M., Galleni M., Quinting B., Frere J.-M., Charlier P.
      Submitted (SEP-1998) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
      Strain: D316.

    Entry informationi

    Entry nameiBLAF_MYCFO
    AccessioniPrimary (citable) accession number: Q59517
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3