Q59498 (ILVB_MYCAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase
EC=2.2.1.6

Alternative name(s):
ALS
Acetohydroxy-acid synthase

Gene names

Name:

ilvB

Organism

Mycobacterium avium

Taxonomic identifier

1764 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Acetolactate synthase		PRO_0000090800
Regions
	Nucleotide binding	296 – 317	22	FAD By similarity
	Nucleotide binding	339 – 358	20	FAD By similarity
	Region	432 – 512	81	Thiamine pyrophosphate binding
Sites
	Metal binding	483	1	Magnesium By similarity
	Metal binding	510	1	Magnesium By similarity
	Binding site	89	1	Thiamine pyrophosphate By similarity
	Binding site	190	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59498 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F998E18CF893CF96	FASTA	621	65,913
	10 20 30 40 50 60 MSAPTRRPAP DAPGAAGIAP APPAPAAKPA AGKPKRIGPE QVTGAQSVIR SLEELGVEVI 70 80 90 100 110 120 FGIPGGAVLP VYDPLFDSKK LRHVLVRHEQ GAGHAASGYA HATGKVGVCM ATSGPGATNL 130 140 150 160 170 180 VTALADAQMD SIPVVAVTGQ VGRTLIGTDA FQEADISGIT MPITKHNFLV VRQRNPAVLA 190 200 210 220 230 240 EAFHIAASGR PARCSVDIPK DVLQGQCTFS WPPRIHLPGY KPTTKPHSRQ IRERAKLIAA 250 260 270 280 290 300 ARKPVLYVGG GVIRGEASEQ LRELAELTGI PVVTTLMARG AFPDSHRQHL GMPGMHGTVA 310 320 330 340 350 360 AVAALQRSDL LIALGTRFDD RVTGKLDTFA PEAKVIHADI DPAEIGKNRH ADVPIVGDVK 370 380 390 400 410 420 AVIAELVEIL RHDGAPGNLD IADWWAYLDD VQSTYPLSYG PQSDGSLGPE YVIEKLGQIA 430 440 450 460 470 480 GPDALYVAGV GHDQMWAAQF ISYEKPRTWL NSGGQGTMGF AIPAAMGAKM GRPEAEVWAI 490 500 510 520 530 540 DGDGCFQMTN QELATCAVEG IPIKVALINN GNLGMVRQWQ TLFYEERYSQ TDLGHPLAPH 550 560 570 580 590 600 PDFVKLAEAL GCVGLRCERE EDVVDVINAA RAINDRPVVI AFIVGADAQV WPMVAAGTSN 610 620 DEIQAARGIR PLFDDETEGT P

References

[1]	"Cloning and sequencing of the ilvBNC gene cluster from Mycobacterium avium." Gusberti L., Cantoni R., de Rossi E., Branzoni M., Riccardi G. Gene 177:83-85(1996) [PubMed: 8921849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L49392 Genomic DNA. Translation: AAB38426.1.
PIR	JC5164.
3D structure databases
ProteinModelPortal	Q59498.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. Acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_MYCAV

Accession

Primary (citable) accession number: Q59498

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program