Q59487 (DYR_LACLA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 82.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Dihydrofolate reductase EC=1.5.1.3 | ||||||||
| Gene names |
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| Organism | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) | ||||||||
| Taxonomic identifier | 272623 [NCBI] | ||||||||
| Taxonomic lineage | Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus |
Protein attributes
| Sequence length | 168 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity. |
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. |
| Pathway | |
| Sequence similarities | Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic processInferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 168 | 168 | Dihydrofolate reductase | PRO_0000186394 | |||||
Regions | |||||||||
| Domain | 1 – 164 | 164 | DHFR | ||||||
| Nucleotide binding | 6 – 7 | 2 | NADP By similarity | ||||||
| Nucleotide binding | 14 – 19 | 6 | NADP By similarity | ||||||
| Nucleotide binding | 43 – 46 | 4 | NADP By similarity | ||||||
| Nucleotide binding | 63 – 66 | 4 | NADP By similarity | ||||||
| Nucleotide binding | 99 – 104 | 6 | NADP By similarity | ||||||
| Region | 5 – 7 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 27 | 1 | Substrate By similarity | ||||||
| Binding site | 58 | 1 | Substrate By similarity | ||||||
| Binding site | 118 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 11 | 1 | A → Q in CAA43094. Ref.1 | ||||||
| Sequence conflict | 29 | 1 | Q → K in CAA43094. Ref.1 | ||||||
| Sequence conflict | 72 | 1 | D → E in CAA43094. Ref.1 | ||||||
| Sequence conflict | 90 | 1 | H → Y in CAA43094. Ref.1 | ||||||
| Sequence conflict | 93 | 1 | N → D in CAA43094. Ref.1 | ||||||
| Sequence conflict | 125 | 1 | K → Q in CAA43094. Ref.1 | ||||||
| Sequence conflict | 132 – 133 | 2 | ST → TH in CAA43094. Ref.1 | ||||||
| Sequence conflict | 138 | 1 | R → K in CAA43094. Ref.1 | ||||||
| Sequence conflict | 141 | 1 | L → V in CAA43094. Ref.1 | ||||||
| Sequence conflict | 145 | 1 | K → I in CAA43094. Ref.1 | ||||||
| Sequence conflict | 154 | 1 | S → A in CAA43094. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis." Leszczynska K., Bolhuis A., Leenhouts K., Venema G., Ceglowski P. Appl. Environ. Microbiol. 61:561-566(1995) [PubMed: 7574597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 51-MG/7. |
| [2] | "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X60681 Genomic DNA. Translation: CAA43094.1. AE005176 Genomic DNA. Translation: AAK05248.1. |
| PIR | F86768. S47467. |
| RefSeq | NP_267306.1. NC_002662.1. |
3D structure databases | |
| ProteinModelPortal | Q59487. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1114791. |
| GenomeReviews | Gene locus LL1150 in contig AE005176_GR. |
| KEGG | lla:L162872. |
| NMPDR | fig|272623.1.peg.1183. |
| PATRIC | 22294642. VBILacLac136773_1229. |
Phylogenomic databases | |
| HOGENOM | HBG665708. |
| OMA | QDEEGVI. |
| ProtClustDB | CLSK797157. |
Enzyme and pathway databases | |
| BioCyc | LLAC272623:L162872-MONOMER. |
Family and domain databases | |
| InterPro | IPR012259. DHFR. IPR024072. DHFR-like_dom. IPR017925. DHFR_CS. IPR001796. DHFR_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit. |
| KO | K00287. |
| PANTHER | PTHR11549:SF1. DHFR. 1 hit. |
| Pfam | PF00186. DHFR_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF000194. DHFR. 1 hit. |
| PRINTS | PR00070. DHFR. |
| SUPFAM | SSF53597. SSF53597. 1 hit. |
| PROSITE | PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DYR_LACLA | ||||||||
| Accession | Primary (citable) accession number: Q59487 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with