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Protein

Dihydrofolate reductase

Gene

folA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateBy similarity1
Binding sitei58SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 7NADPBy similarity2
Nucleotide bindingi14 – 19NADPBy similarity6
Nucleotide bindingi43 – 46NADPBy similarity4
Nucleotide bindingi63 – 66NADPBy similarity4
Nucleotide bindingi99 – 104NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciLLAC272623:G1FZA-1220-MONOMER
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:LL1150
ORF Names:L162872
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4012

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863941 – 168Dihydrofolate reductaseAdd BLAST168

Proteomic databases

PaxDbiQ59487

Interactioni

Protein-protein interaction databases

STRINGi272623.L162872

Chemistry databases

BindingDBiQ59487

Structurei

3D structure databases

ProteinModelPortaliQ59487
SMRiQ59487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 164DHFRPROSITE-ProRule annotationAdd BLAST164

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 7Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108YYV Bacteria
COG0262 LUCA
HOGENOMiHOG000040233
KOiK00287
OMAiRDNQLPW

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q59487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIGIWAEDE AGLIGEADKM PWSLPAEQQH FKETTMNQVI LMGRKTFEGM
60 70 80 90 100
NKRVLPGRIS IILTRDETYQ SDNEKVLIMH SPKEVLDWYH KQNKDLFITG
110 120 130 140 150
GAEILALFES ELELLYRTVV HEKFKGDTYF PSTFDFGRFK LVSEKFHDKD
160
ERNSYTFTIK KYEKVKQP
Length:168
Mass (Da):19,729
Last modified:April 27, 2001 - v2
Checksum:i0BB4D97167F247F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → Q in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti29Q → K in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti72D → E in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti90H → Y in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti93N → D in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti125K → Q in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti132 – 133ST → TH in CAA43094 (PubMed:7574597).Curated2
Sequence conflicti138R → K in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti141L → V in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti145K → I in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti154S → A in CAA43094 (PubMed:7574597).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60681 Genomic DNA Translation: CAA43094.1
AE005176 Genomic DNA Translation: AAK05248.1
PIRiF86768
S47467
RefSeqiNP_267306.1, NC_002662.1
WP_010905786.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK05248; AAK05248; L162872
GeneIDi1114791
KEGGilla:L162872
PATRICifig|272623.7.peg.1229

Similar proteinsi

Entry informationi

Entry nameiDYR_LACLA
AccessioniPrimary (citable) accession number: Q59487
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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