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Q59487

- DYR_LACLA

UniProt

Q59487 - DYR_LACLA

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Protein

Dihydrofolate reductase

Gene

folA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271SubstrateBy similarity
Binding sitei58 – 581SubstrateBy similarity
Binding sitei118 – 1181SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 72NADPBy similarity
Nucleotide bindingi14 – 196NADPBy similarity
Nucleotide bindingi43 – 464NADPBy similarity
Nucleotide bindingi63 – 664NADPBy similarity
Nucleotide bindingi99 – 1046NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1231-MONOMER.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:LL1150
ORF Names:L162872
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Dihydrofolate reductasePRO_0000186394Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L162872.

Structurei

3D structure databases

ProteinModelPortaliQ59487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 164164DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 73Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040233.
KOiK00287.
OMAiIGIMAVD.
OrthoDBiEOG6KT2V2.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59487-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIIGIWAEDE AGLIGEADKM PWSLPAEQQH FKETTMNQVI LMGRKTFEGM
60 70 80 90 100
NKRVLPGRIS IILTRDETYQ SDNEKVLIMH SPKEVLDWYH KQNKDLFITG
110 120 130 140 150
GAEILALFES ELELLYRTVV HEKFKGDTYF PSTFDFGRFK LVSEKFHDKD
160
ERNSYTFTIK KYEKVKQP
Length:168
Mass (Da):19,729
Last modified:April 27, 2001 - v2
Checksum:i0BB4D97167F247F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111A → Q in CAA43094. (PubMed:7574597)Curated
Sequence conflicti29 – 291Q → K in CAA43094. (PubMed:7574597)Curated
Sequence conflicti72 – 721D → E in CAA43094. (PubMed:7574597)Curated
Sequence conflicti90 – 901H → Y in CAA43094. (PubMed:7574597)Curated
Sequence conflicti93 – 931N → D in CAA43094. (PubMed:7574597)Curated
Sequence conflicti125 – 1251K → Q in CAA43094. (PubMed:7574597)Curated
Sequence conflicti132 – 1332ST → TH in CAA43094. (PubMed:7574597)Curated
Sequence conflicti138 – 1381R → K in CAA43094. (PubMed:7574597)Curated
Sequence conflicti141 – 1411L → V in CAA43094. (PubMed:7574597)Curated
Sequence conflicti145 – 1451K → I in CAA43094. (PubMed:7574597)Curated
Sequence conflicti154 – 1541S → A in CAA43094. (PubMed:7574597)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60681 Genomic DNA. Translation: CAA43094.1.
AE005176 Genomic DNA. Translation: AAK05248.1.
PIRiF86768.
S47467.
RefSeqiNP_267306.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05248; AAK05248; L162872.
GeneIDi1114791.
KEGGilla:L162872.
PATRICi22294642. VBILacLac136773_1229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60681 Genomic DNA. Translation: CAA43094.1 .
AE005176 Genomic DNA. Translation: AAK05248.1 .
PIRi F86768.
S47467.
RefSeqi NP_267306.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q59487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L162872.

Chemistry

ChEMBLi CHEMBL4012.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK05248 ; AAK05248 ; L162872 .
GeneIDi 1114791.
KEGGi lla:L162872.
PATRICi 22294642. VBILacLac136773_1229.

Phylogenomic databases

eggNOGi COG0262.
HOGENOMi HOG000040233.
KOi K00287.
OMAi IGIMAVD.
OrthoDBi EOG6KT2V2.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci LLAC272623:GHSH-1231-MONOMER.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000194. DHFR. 1 hit.
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis."
    Leszczynska K., Bolhuis A., Leenhouts K., Venema G., Ceglowski P.
    Appl. Environ. Microbiol. 61:561-566(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 51-MG/7.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiDYR_LACLA
AccessioniPrimary (citable) accession number: Q59487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 27, 2001
Last modified: October 1, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3