Reviewed,
UniProtKB/Swiss-Prot Q59487 (DYR_LACLA)
Last modified
November 25, 2008.
Version 56.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dihydrofolate reductase EC=1.5.1.3 | ||||||||
| Gene names |
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| Organism | Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP] | ||||||||
| Taxonomic identifier | 1360 [NCBI] | ||||||||
| Taxonomic lineage | Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus |
Protein attributes
| Sequence length | 168 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. |
| Pathway | Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1. |
| Miscellaneous | The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP. |
| Sequence similarities | Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic processInferred from electronic annotation. Source: InterPro one-carbon compound metabolic processInferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 168 | 168 | Dihydrofolate reductase | PRO_0000186394 | |||||
Regions | |||||||||
| Domain | 1 – 164 | 164 | DHFR | ||||||
Experimental info | |||||||||
| Sequence conflict | 11 | 1 | A → Q in CAA43094. Ref.1 | ||||||
| Sequence conflict | 29 | 1 | Q → K in CAA43094. Ref.1 | ||||||
| Sequence conflict | 72 | 1 | D → E in CAA43094. Ref.1 | ||||||
| Sequence conflict | 90 | 1 | H → Y in CAA43094. Ref.1 | ||||||
| Sequence conflict | 93 | 1 | N → D in CAA43094. Ref.1 | ||||||
| Sequence conflict | 125 | 1 | K → Q in CAA43094. Ref.1 | ||||||
| Sequence conflict | 132 – 133 | 2 | ST → TH in CAA43094. Ref.1 | ||||||
| Sequence conflict | 138 | 1 | R → K in CAA43094. Ref.1 | ||||||
| Sequence conflict | 141 | 1 | L → V in CAA43094. Ref.1 | ||||||
| Sequence conflict | 145 | 1 | K → I in CAA43094. Ref.1 | ||||||
| Sequence conflict | 154 | 1 | S → A in CAA43094. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis." Leszczynska K., Bolhuis A., Leenhouts K., Venema G., Ceglowski P. Appl. Environ. Microbiol. 61:561-566(1995) [PubMed: 7574597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 51-MG/7. |
| [2] | "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403. |
Cross-references
Sequence databases | |
|---|---|
| X60681 Genomic DNA. Translation: CAA43094.1. AE006347 Genomic DNA. Translation: AAK05248.1. | |
| PIR | F86768. S47467. |
| RefSeq | NP_267306.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DHI based on UniProtKB P00379. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1114791. |
| GenomeReviews | Gene locus LL1150 in contig AE005176_GR. |
| KEGG | lla:L162872. |
| NMPDR | fig|272623.1.peg.1183. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q59487. |
Enzyme and pathway databases | |
| BioCyc | LLAC272623:L162872-MON. |
Family and domain databases | |
| InterPro | IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view] |
| PANTHER | PTHR11549:SF1. DHFR. 1 hit. |
| Pfam | PF00186. DHFR_1. 1 hit. [Graphical view] |
| PRINTS | PR00070. DHFR. |
| PROSITE | PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DYR_LACLA | ||||||||
| Accession | Primary (citable) accession number: Q59487 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


