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Protein

Dihydrofolate reductase

Gene

folA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateBy similarity1
Binding sitei58SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 7NADPBy similarity2
Nucleotide bindingi14 – 19NADPBy similarity6
Nucleotide bindingi43 – 46NADPBy similarity4
Nucleotide bindingi63 – 66NADPBy similarity4
Nucleotide bindingi99 – 104NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:LL1150
ORF Names:L162872
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4012.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863941 – 168Dihydrofolate reductaseAdd BLAST168

Proteomic databases

PaxDbiQ59487.

Interactioni

Protein-protein interaction databases

STRINGi272623.L162872.

Chemistry databases

BindingDBiQ59487.

Structurei

3D structure databases

ProteinModelPortaliQ59487.
SMRiQ59487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 164DHFRPROSITE-ProRule annotationAdd BLAST164

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 7Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.
HOGENOMiHOG000040233.
KOiK00287.
OMAiVTFEGMK.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIGIWAEDE AGLIGEADKM PWSLPAEQQH FKETTMNQVI LMGRKTFEGM
60 70 80 90 100
NKRVLPGRIS IILTRDETYQ SDNEKVLIMH SPKEVLDWYH KQNKDLFITG
110 120 130 140 150
GAEILALFES ELELLYRTVV HEKFKGDTYF PSTFDFGRFK LVSEKFHDKD
160
ERNSYTFTIK KYEKVKQP
Length:168
Mass (Da):19,729
Last modified:April 27, 2001 - v2
Checksum:i0BB4D97167F247F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → Q in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti29Q → K in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti72D → E in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti90H → Y in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti93N → D in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti125K → Q in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti132 – 133ST → TH in CAA43094 (PubMed:7574597).Curated2
Sequence conflicti138R → K in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti141L → V in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti145K → I in CAA43094 (PubMed:7574597).Curated1
Sequence conflicti154S → A in CAA43094 (PubMed:7574597).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60681 Genomic DNA. Translation: CAA43094.1.
AE005176 Genomic DNA. Translation: AAK05248.1.
PIRiF86768.
S47467.
RefSeqiNP_267306.1. NC_002662.1.
WP_010905786.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05248; AAK05248; L162872.
GeneIDi1114791.
KEGGilla:L162872.
PATRICi22294642. VBILacLac136773_1229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60681 Genomic DNA. Translation: CAA43094.1.
AE005176 Genomic DNA. Translation: AAK05248.1.
PIRiF86768.
S47467.
RefSeqiNP_267306.1. NC_002662.1.
WP_010905786.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ59487.
SMRiQ59487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L162872.

Chemistry databases

BindingDBiQ59487.
ChEMBLiCHEMBL4012.

Proteomic databases

PaxDbiQ59487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05248; AAK05248; L162872.
GeneIDi1114791.
KEGGilla:L162872.
PATRICi22294642. VBILacLac136773_1229.

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.
HOGENOMiHOG000040233.
KOiK00287.
OMAiVTFEGMK.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYR_LACLA
AccessioniPrimary (citable) accession number: Q59487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.