Reviewed, UniProtKB/Swiss-Prot Q59487 (DYR_LACLA)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	dhfR
Ordered Locus Names:	LL1150
ORF Names:	L162872

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	Dihydrofolate reductase		PRO_0000186394
Regions
	Domain	1 – 164	164	DHFR
Experimental info
	Sequence conflict	11	1	A → Q in CAA43094. Ref.1
	Sequence conflict	29	1	Q → K in CAA43094. Ref.1
	Sequence conflict	72	1	D → E in CAA43094. Ref.1
	Sequence conflict	90	1	H → Y in CAA43094. Ref.1
	Sequence conflict	93	1	N → D in CAA43094. Ref.1
	Sequence conflict	125	1	K → Q in CAA43094. Ref.1
	Sequence conflict	132 – 133	2	ST → TH in CAA43094. Ref.1
	Sequence conflict	138	1	R → K in CAA43094. Ref.1
	Sequence conflict	141	1	L → V in CAA43094. Ref.1
	Sequence conflict	145	1	K → I in CAA43094. Ref.1
	Sequence conflict	154	1	S → A in CAA43094. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59487-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 0BB4D97167F247F4	FASTA	168	19,729
	10 20 30 40 50 60 MIIGIWAEDE AGLIGEADKM PWSLPAEQQH FKETTMNQVI LMGRKTFEGM NKRVLPGRIS 70 80 90 100 110 120 IILTRDETYQ SDNEKVLIMH SPKEVLDWYH KQNKDLFITG GAEILALFES ELELLYRTVV 130 140 150 160 HEKFKGDTYF PSTFDFGRFK LVSEKFHDKD ERNSYTFTIK KYEKVKQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis." Leszczynska K., Bolhuis A., Leenhouts K., Venema G., Ceglowski P. Appl. Environ. Microbiol. 61:561-566(1995) [PubMed: 7574597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 51-MG/7.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60681 Genomic DNA. Translation: CAA43094.1. AE006347 Genomic DNA. Translation: AAK05248.1.
PIR	F86768. S47467.
RefSeq	NP_267306.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHI based on UniProtKB P00379.
ModBase	Search...
Genome annotation databases
GeneID	1114791.
GenomeReviews	Gene locus LL1150 in contig AE005176_GR.
KEGG	lla:L162872.
NMPDR	fig|272623.1.peg.1183.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59487.
Enzyme and pathway databases
BioCyc	LLAC272623:L162872-MON.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_LACLA

Accession

Primary (citable) accession number: Q59487

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)