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Q59471

- Q59471_KLEOX

UniProt

Q59471 - Q59471_KLEOX

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Protein
Submitted name: Diol dehydrase beta subunit
Gene
pddB
Organism
Klebsiella oxytoca
Status
Unreviewed - Annotation score: 1 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei174 – 1741PhosphateImported
Binding sitei182 – 1821Phosphate; via amide nitrogenImported

GO - Molecular functioni

  1. propanediol dehydratase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Names & Taxonomyi

Protein namesi
Submitted name:
Diol dehydrase beta subunitImported (EC:4.2.1.28Imported)
Gene namesi
Name:pddBImported
OrganismiKlebsiella oxytocaImported
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Interactioni

Protein-protein interaction databases

IntActiQ59471. 2 interactions.
MINTiMINT-7997115.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DIOX-ray2.20B/E1-224[»]
1EEXX-ray1.70B/E1-224[»]
1EGMX-ray1.85B/E1-224[»]
1EGVX-ray1.75B/E1-224[»]
1IWBX-ray1.85B/E1-224[»]
1UC4X-ray1.80B/E1-224[»]
1UC5X-ray2.30B/E1-224[»]
3AUJX-ray2.10B/E1-224[»]
ProteinModelPortaliQ59471.
SMRiQ59471. Positions 46-224.

Miscellaneous databases

EvolutionaryTraceiQ59471.

Family & Domainsi

Phylogenomic databases

KOiK13919.

Family and domain databases

Gene3Di3.40.50.10150. 1 hit.
InterProiIPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view]
PfamiPF02288. Dehydratase_MU. 1 hit.
[Graphical view]
PIRSFiPIRSF018506. Prpndl_dhdrts_md. 1 hit.
SUPFAMiSSF52968. SSF52968. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59471-1 [UniParc]FASTAAdd to Basket

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MEINEKLLRQ IIEDVLSEMK GSDKPVSFNA PAASAAPQAT PPAGDGFLTE    50
VGEARQGTQQ DEVIIAVGPA FGLAQTVNIV GIPHKSILRE VIAGIEEEGI 100
KARVIRCFKS SDVAFVAVEG NRLSGSGISI GIQSKGTTVI HQQGLPPLSN 150
LELFPQAPLL TLETYRQIGK NAARYAKRES PQPVPTLNDQ MARPKYQAKS 200
AILHIKETKY VVTGKNPQEL RVAL 224
Length:224
Mass (Da):24,114
Last modified:November 1, 1996 - v1
Checksum:iC0A7C45A55CFAC7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45071 Genomic DNA. Translation: BAA08100.1.
PIRiB56111.
RefSeqiYP_005016276.1. NC_016612.1.

Genome annotation databases

GeneIDi11660426.
KEGGikox:KOX_01460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45071 Genomic DNA. Translation: BAA08100.1 .
PIRi B56111.
RefSeqi YP_005016276.1. NC_016612.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DIO X-ray 2.20 B/E 1-224 [» ]
1EEX X-ray 1.70 B/E 1-224 [» ]
1EGM X-ray 1.85 B/E 1-224 [» ]
1EGV X-ray 1.75 B/E 1-224 [» ]
1IWB X-ray 1.85 B/E 1-224 [» ]
1UC4 X-ray 1.80 B/E 1-224 [» ]
1UC5 X-ray 2.30 B/E 1-224 [» ]
3AUJ X-ray 2.10 B/E 1-224 [» ]
ProteinModelPortali Q59471.
SMRi Q59471. Positions 46-224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q59471. 2 interactions.
MINTi MINT-7997115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11660426.
KEGGi kox:KOX_01460.

Phylogenomic databases

KOi K13919.

Miscellaneous databases

EvolutionaryTracei Q59471.

Family and domain databases

Gene3Di 3.40.50.10150. 1 hit.
InterProi IPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view ]
Pfami PF02288. Dehydratase_MU. 1 hit.
[Graphical view ]
PIRSFi PIRSF018506. Prpndl_dhdrts_md. 1 hit.
SUPFAMi SSF52968. SSF52968. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca."
    Tobimatsu T., Hara T., Sakaguchi M., Kishimoto Y., Wada Y., Isoda M., Sakai T., Toraya T.
    J. Biol. Chem. 270:7142-7148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 8724Imported.
  2. "A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase."
    Shibata N., Masuda J., Tobimatsu T., Toraya T., Suto K., Morimoto Y., Yasuoka N.
    Structure 7:997-1008(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
  3. "How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex."
    Masuda J., Shibata N., Morimoto Y., Toraya T., Yasuoka N.
    Structure 8:775-788(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
  4. "Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase."
    Shibata N., Masuda J., Morimoto Y., Yasuoka N., Toraya T.
    Biochemistry 41:12607-12617(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
  5. "Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."
    Shibata N., Nakanishi Y., Fukuoka M., Yamanishi M., Yasuoka N., Toraya T.
    J. Biol. Chem. 278:22717-22725(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
  6. "Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols."
    Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T.
    FEBS J. 279:793-804(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PHOSPHATE.

Entry informationi

Entry nameiQ59471_KLEOX
AccessioniPrimary (citable) accession number: Q59471
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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