Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q59471

- Q59471_KLEOX

UniProt

Q59471 - Q59471_KLEOX

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Submitted name:

Diol dehydrase beta subunit

Gene

pddB

Organism
Klebsiella oxytoca
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei174 – 1741PhosphateImported
Binding sitei182 – 1821Phosphate; via amide nitrogenImported

GO - Molecular functioni

  1. propanediol dehydratase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Names & Taxonomyi

Protein namesi
Submitted name:
Diol dehydrase beta subunitImported (EC:4.2.1.28Imported)
Gene namesi
Name:pddBImported
OrganismiKlebsiella oxytocaImported
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Interactioni

Protein-protein interaction databases

IntActiQ59471. 2 interactions.
MINTiMINT-7997115.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DIOX-ray2.20B/E1-224[»]
1EEXX-ray1.70B/E1-224[»]
1EGMX-ray1.85B/E1-224[»]
1EGVX-ray1.75B/E1-224[»]
1IWBX-ray1.85B/E1-224[»]
1UC4X-ray1.80B/E1-224[»]
1UC5X-ray2.30B/E1-224[»]
3AUJX-ray2.10B/E1-224[»]
ProteinModelPortaliQ59471.
SMRiQ59471. Positions 46-224.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59471.

Family & Domainsi

Phylogenomic databases

KOiK13919.

Family and domain databases

Gene3Di3.40.50.10150. 1 hit.
InterProiIPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view]
PfamiPF02288. Dehydratase_MU. 1 hit.
[Graphical view]
PIRSFiPIRSF018506. Prpndl_dhdrts_md. 1 hit.
SUPFAMiSSF52968. SSF52968. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEINEKLLRQ IIEDVLSEMK GSDKPVSFNA PAASAAPQAT PPAGDGFLTE
60 70 80 90 100
VGEARQGTQQ DEVIIAVGPA FGLAQTVNIV GIPHKSILRE VIAGIEEEGI
110 120 130 140 150
KARVIRCFKS SDVAFVAVEG NRLSGSGISI GIQSKGTTVI HQQGLPPLSN
160 170 180 190 200
LELFPQAPLL TLETYRQIGK NAARYAKRES PQPVPTLNDQ MARPKYQAKS
210 220
AILHIKETKY VVTGKNPQEL RVAL
Length:224
Mass (Da):24,114
Last modified:November 1, 1996 - v1
Checksum:iC0A7C45A55CFAC7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45071 Genomic DNA. Translation: BAA08100.1.
PIRiB56111.
RefSeqiYP_005016276.1. NC_016612.1.

Genome annotation databases

GeneIDi11660426.
KEGGikox:KOX_01460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45071 Genomic DNA. Translation: BAA08100.1 .
PIRi B56111.
RefSeqi YP_005016276.1. NC_016612.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DIO X-ray 2.20 B/E 1-224 [» ]
1EEX X-ray 1.70 B/E 1-224 [» ]
1EGM X-ray 1.85 B/E 1-224 [» ]
1EGV X-ray 1.75 B/E 1-224 [» ]
1IWB X-ray 1.85 B/E 1-224 [» ]
1UC4 X-ray 1.80 B/E 1-224 [» ]
1UC5 X-ray 2.30 B/E 1-224 [» ]
3AUJ X-ray 2.10 B/E 1-224 [» ]
ProteinModelPortali Q59471.
SMRi Q59471. Positions 46-224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q59471. 2 interactions.
MINTi MINT-7997115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11660426.
KEGGi kox:KOX_01460.

Phylogenomic databases

KOi K13919.

Miscellaneous databases

EvolutionaryTracei Q59471.

Family and domain databases

Gene3Di 3.40.50.10150. 1 hit.
InterProi IPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view ]
Pfami PF02288. Dehydratase_MU. 1 hit.
[Graphical view ]
PIRSFi PIRSF018506. Prpndl_dhdrts_md. 1 hit.
SUPFAMi SSF52968. SSF52968. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca."
    Tobimatsu T., Hara T., Sakaguchi M., Kishimoto Y., Wada Y., Isoda M., Sakai T., Toraya T.
    J. Biol. Chem. 270:7142-7148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 8724Imported.
  2. "A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase."
    Shibata N., Masuda J., Tobimatsu T., Toraya T., Suto K., Morimoto Y., Yasuoka N.
    Structure 7:997-1008(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
  3. "How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex."
    Masuda J., Shibata N., Morimoto Y., Toraya T., Yasuoka N.
    Structure 8:775-788(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
  4. "Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase."
    Shibata N., Masuda J., Morimoto Y., Yasuoka N., Toraya T.
    Biochemistry 41:12607-12617(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
  5. "Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."
    Shibata N., Nakanishi Y., Fukuoka M., Yamanishi M., Yasuoka N., Toraya T.
    J. Biol. Chem. 278:22717-22725(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
  6. "Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols."
    Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T.
    FEBS J. 279:793-804(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).

Entry informationi

Entry nameiQ59471_KLEOX
AccessioniPrimary (citable) accession number: Q59471
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3