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Q59471

- Q59471_KLEOX

UniProt

Q59471 - Q59471_KLEOX

Protein
Submitted name:

Diol dehydrase beta subunit

Gene

pddB

Organism
Klebsiella oxytoca
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei174 – 1741PhosphateImported
    Binding sitei182 – 1821Phosphate; via amide nitrogenImported

    GO - Molecular functioni

    1. propanediol dehydratase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    LyaseImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Diol dehydrase beta subunitImported (EC:4.2.1.28Imported)
    Gene namesi
    Name:pddBImported
    OrganismiKlebsiella oxytocaImported
    Taxonomic identifieri571 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    Interactioni

    Protein-protein interaction databases

    IntActiQ59471. 2 interactions.
    MINTiMINT-7997115.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DIOX-ray2.20B/E1-224[»]
    1EEXX-ray1.70B/E1-224[»]
    1EGMX-ray1.85B/E1-224[»]
    1EGVX-ray1.75B/E1-224[»]
    1IWBX-ray1.85B/E1-224[»]
    1UC4X-ray1.80B/E1-224[»]
    1UC5X-ray2.30B/E1-224[»]
    3AUJX-ray2.10B/E1-224[»]
    ProteinModelPortaliQ59471.
    SMRiQ59471. Positions 46-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ59471.

    Family & Domainsi

    Phylogenomic databases

    KOiK13919.

    Family and domain databases

    Gene3Di3.40.50.10150. 1 hit.
    InterProiIPR010254. B12-dep_deHydtase_bsu.
    IPR003208. Dehydtase/Dehydtase_re.
    IPR025541. Ppandiol/glycerol_DHydtase_msu.
    [Graphical view]
    PfamiPF02288. Dehydratase_MU. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018506. Prpndl_dhdrts_md. 1 hit.
    SUPFAMiSSF52968. SSF52968. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q59471-1 [UniParc]FASTAAdd to Basket

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    MEINEKLLRQ IIEDVLSEMK GSDKPVSFNA PAASAAPQAT PPAGDGFLTE    50
    VGEARQGTQQ DEVIIAVGPA FGLAQTVNIV GIPHKSILRE VIAGIEEEGI 100
    KARVIRCFKS SDVAFVAVEG NRLSGSGISI GIQSKGTTVI HQQGLPPLSN 150
    LELFPQAPLL TLETYRQIGK NAARYAKRES PQPVPTLNDQ MARPKYQAKS 200
    AILHIKETKY VVTGKNPQEL RVAL 224
    Length:224
    Mass (Da):24,114
    Last modified:November 1, 1996 - v1
    Checksum:iC0A7C45A55CFAC7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45071 Genomic DNA. Translation: BAA08100.1.
    PIRiB56111.
    RefSeqiYP_005016276.1. NC_016612.1.

    Genome annotation databases

    GeneIDi11660426.
    KEGGikox:KOX_01460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45071 Genomic DNA. Translation: BAA08100.1 .
    PIRi B56111.
    RefSeqi YP_005016276.1. NC_016612.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DIO X-ray 2.20 B/E 1-224 [» ]
    1EEX X-ray 1.70 B/E 1-224 [» ]
    1EGM X-ray 1.85 B/E 1-224 [» ]
    1EGV X-ray 1.75 B/E 1-224 [» ]
    1IWB X-ray 1.85 B/E 1-224 [» ]
    1UC4 X-ray 1.80 B/E 1-224 [» ]
    1UC5 X-ray 2.30 B/E 1-224 [» ]
    3AUJ X-ray 2.10 B/E 1-224 [» ]
    ProteinModelPortali Q59471.
    SMRi Q59471. Positions 46-224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q59471. 2 interactions.
    MINTi MINT-7997115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 11660426.
    KEGGi kox:KOX_01460.

    Phylogenomic databases

    KOi K13919.

    Miscellaneous databases

    EvolutionaryTracei Q59471.

    Family and domain databases

    Gene3Di 3.40.50.10150. 1 hit.
    InterProi IPR010254. B12-dep_deHydtase_bsu.
    IPR003208. Dehydtase/Dehydtase_re.
    IPR025541. Ppandiol/glycerol_DHydtase_msu.
    [Graphical view ]
    Pfami PF02288. Dehydratase_MU. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018506. Prpndl_dhdrts_md. 1 hit.
    SUPFAMi SSF52968. SSF52968. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca."
      Tobimatsu T., Hara T., Sakaguchi M., Kishimoto Y., Wada Y., Isoda M., Sakai T., Toraya T.
      J. Biol. Chem. 270:7142-7148(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 8724Imported.
    2. "A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase."
      Shibata N., Masuda J., Tobimatsu T., Toraya T., Suto K., Morimoto Y., Yasuoka N.
      Structure 7:997-1008(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
    3. "How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex."
      Masuda J., Shibata N., Morimoto Y., Toraya T., Yasuoka N.
      Structure 8:775-788(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
    4. "Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase."
      Shibata N., Masuda J., Morimoto Y., Yasuoka N., Toraya T.
      Biochemistry 41:12607-12617(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
    5. "Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."
      Shibata N., Nakanishi Y., Fukuoka M., Yamanishi M., Yasuoka N., Toraya T.
      J. Biol. Chem. 278:22717-22725(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
    6. "Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols."
      Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T.
      FEBS J. 279:793-804(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).

    Entry informationi

    Entry nameiQ59471_KLEOX
    AccessioniPrimary (citable) accession number: Q59471
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3