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Q59471 (Q59471_KLEOX) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
EC=4.2.1.28 EMBL BAA08100.1
Gene names
Name:pddB EMBL BAA08100.1
OrganismKlebsiella oxytoca EMBL BAA08100.1
Taxonomic identifier571 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site1741Phosphate PDB 3AUJ
Binding site1821Phosphate; via amide nitrogen PDB 3AUJ

Sequences

Sequence LengthMass (Da)Tools
Q59471 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C0A7C45A55CFAC7D

FASTA22424,114
        10         20         30         40         50         60 
MEINEKLLRQ IIEDVLSEMK GSDKPVSFNA PAASAAPQAT PPAGDGFLTE VGEARQGTQQ 

        70         80         90        100        110        120 
DEVIIAVGPA FGLAQTVNIV GIPHKSILRE VIAGIEEEGI KARVIRCFKS SDVAFVAVEG 

       130        140        150        160        170        180 
NRLSGSGISI GIQSKGTTVI HQQGLPPLSN LELFPQAPLL TLETYRQIGK NAARYAKRES 

       190        200        210        220 
PQPVPTLNDQ MARPKYQAKS AILHIKETKY VVTGKNPQEL RVAL 

« Hide

References

[1]"Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca."
Tobimatsu T., Hara T., Sakaguchi M., Kishimoto Y., Wada Y., Isoda M., Sakai T., Toraya T.
J. Biol. Chem. 270:7142-7148(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 8724 EMBL BAA08100.1.
[2]"A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase."
Shibata N., Masuda J., Tobimatsu T., Toraya T., Suto K., Morimoto Y., Yasuoka N.
Structure 7:997-1008(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
[3]"How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex."
Masuda J., Shibata N., Morimoto Y., Toraya T., Yasuoka N.
Structure 8:775-788(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
[4]"Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase."
Shibata N., Masuda J., Morimoto Y., Yasuoka N., Toraya T.
Biochemistry 41:12607-12617(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
[5]"Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase."
Shibata N., Nakanishi Y., Fukuoka M., Yamanishi M., Yasuoka N., Toraya T.
J. Biol. Chem. 278:22717-22725(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
[6]"Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols."
Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T.
FEBS J. 279:793-804(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PHOSPHATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D45071 Genomic DNA. Translation: BAA08100.1.
PIRB56111.
RefSeqYP_005016276.1. NC_016612.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DIOX-ray2.20B/E1-224[»]
1EEXX-ray1.70B/E1-224[»]
1EGMX-ray1.85B/E1-224[»]
1EGVX-ray1.75B/E1-224[»]
1IWBX-ray1.85B/E1-224[»]
1UC4X-ray1.80B/E1-224[»]
1UC5X-ray2.30B/E1-224[»]
3AUJX-ray2.10B/E1-224[»]
ProteinModelPortalQ59471.
SMRQ59471. Positions 46-224.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ59471. 2 interactions.
MINTMINT-7997115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11660426.
KEGGkox:KOX_01460.

Phylogenomic databases

KOK13919.

Family and domain databases

Gene3D3.40.50.10150. 1 hit.
InterProIPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view]
PfamPF02288. Dehydratase_MU. 1 hit.
[Graphical view]
PIRSFPIRSF018506. Prpndl_dhdrts_md. 1 hit.
SUPFAMSSF52968. SSF52968. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ59471.

Entry information

Entry nameQ59471_KLEOX
AccessionPrimary (citable) accession number: Q59471
Entry history
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)