Q59471 (Q59471_KLEOX) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Diol dehydrase beta subunit EMBL BAA08100.1
EC=4.2.1.28 EMBL BAA08100.1

Gene names

Name:

pddB EMBL BAA08100.1

Organism

Klebsiella oxytoca EMBL BAA08100.1

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Lyase EMBL BAA08100.1
Technical term	3D-structure PDB 1UC5 PDB 1IWB PDB 1EGV PDB 1EGM PDB 3AUJ PDB 1DIO PDB 1EEX PDB 1UC4
Gene Ontology (GO)
Molecular_function	propanediol dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q59471 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C0A7C45A55CFAC7D	FASTA	224	24,114
	10 20 30 40 50 60 MEINEKLLRQ IIEDVLSEMK GSDKPVSFNA PAASAAPQAT PPAGDGFLTE VGEARQGTQQ 70 80 90 100 110 120 DEVIIAVGPA FGLAQTVNIV GIPHKSILRE VIAGIEEEGI KARVIRCFKS SDVAFVAVEG 130 140 150 160 170 180 NRLSGSGISI GIQSKGTTVI HQQGLPPLSN LELFPQAPLL TLETYRQIGK NAARYAKRES 190 200 210 220 PQPVPTLNDQ MARPKYQAKS AILHIKETKY VVTGKNPQEL RVAL

References

[1]	"Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca." Tobimatsu T., Hara T., Sakaguchi M., Kishimoto Y., Wada Y., Isoda M., Sakai T., Toraya T. J. Biol. Chem. 270:7142-7148(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 8724 EMBL BAA08100.1.
[2]	"A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase." Shibata N., Masuda J., Tobimatsu T., Toraya T., Suto K., Morimoto Y., Yasuoka N. Structure 7:997-1008(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
[3]	"How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex." Masuda J., Shibata N., Morimoto Y., Toraya T., Yasuoka N. Structure 8:775-788(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
[4]	"Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase." Shibata N., Masuda J., Morimoto Y., Yasuoka N., Toraya T. Biochemistry 41:12607-12617(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
[5]	"Structural rationalization for the lack of stereospecificity in coenzyme B12-dependent diol dehydratase." Shibata N., Nakanishi Y., Fukuoka M., Yamanishi M., Yasuoka N., Toraya T. J. Biol. Chem. 278:22717-22725(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
[6]	"Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols." Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T. FEBS J. 279:793-804(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D45071 Genomic DNA. Translation: BAA08100.1.

PIR

B56111.

RefSeq

YP_005016276.1. NC_016612.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DIO	X-ray	2.20	B/E	1-224	[»]
1EEX	X-ray	1.70	B/E	1-224	[»]
1EGM	X-ray	1.85	B/E	1-224	[»]
1EGV	X-ray	1.75	B/E	1-224	[»]
1IWB	X-ray	1.85	B/E	1-224	[»]
1UC4	X-ray	1.80	B/E	1-224	[»]
1UC5	X-ray	2.30	B/E	1-224	[»]
3AUJ	X-ray	2.10	B/E	1-224	[»]

ProteinModelPortal

Q59471.

SMR

Q59471. Positions 46-224.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q59471. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

11660426.

KEGG

kox:KOX_01460.

Phylogenomic databases

KO

K13919.

Family and domain databases

Gene3D

3.40.50.10150. 1 hit.

InterPro

IPR010254. B12-dep_deHydtase_bsu.
IPR003208. Dehydtase/Dehydtase_re.
IPR025541. Ppandiol/glycerol_DHydtase_msu.
[Graphical view]

Pfam

PF02288. Dehydratase_MU. 1 hit.
[Graphical view]

PIRSF

PIRSF018506. Prpndl_dhdrts_md. 1 hit.

SUPFAM

SSF52968. B12_dehydrtse_BU. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q59471.

Entry information

Entry name

Q59471_KLEOX

Accession

Primary (citable) accession number: Q59471

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)