Q59468 (HMDH_HALVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Short name=HMG-CoA reductase
EC=1.1.1.34

Gene names

Name:	hmgA
Synonyms:	hmg
Ordered Locus Names:	HVO_2583

Organism

Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Complete proteome] [HAMAP]

Taxonomic identifier

309800 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Induction	Inhibited by mevinolin.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114460
Sites
	Active site	99	1	Charge relay system By similarity
	Active site	303	1	Charge relay system By similarity
	Active site	398	1	Proton donor By similarity
Experimental info
	Sequence conflict	264 – 266	3	AVA → RGR in AAA73174. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59468 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: A85C7F7FE7E94833	FASTA	403	41,085
	10 20 30 40 50 60 MTDAASLADR VREGDLRLHE LEAHADADTA AEARRLLVES QSGASLDAVG NYGFPAEAAE 70 80 90 100 110 120 SAIENMVGSI QVPMGVAGPV SVDGGSVAGE KYLPLATTEG ALLASVNRGC SVINSAGGAT 130 140 150 160 170 180 ARVLKSGMTR APVFRVADVA EAEALVSWTR DNFAALKEAA EETTNHGELL DVTPYVVGNS 190 200 210 220 230 240 VYLRFRYDTK DAMGMNMATI ATEAVCGVVE AETAASLVAL SGNLCSDKKP AAINAVEGRG 250 260 270 280 290 300 RSVTADVRIP REVVEERLHT TPEAVAELNT RKNLVGSAKA ASLGFNAHVA NVVAAMFLAT 310 320 330 340 350 360 GQDEAQVVEG ANAITTAEVQ DGDLYVSVSI ASLEVGTVGG GTKLPTQSEG LDILGVSGGG 370 380 390 400 DPAGSNADAL AECIAVGSLA GELSLLSALA SRHLSSAHAE LGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii." Lam W.L., Doolittle W.F. J. Biol. Chem. 267:5829-5834(1992) [PubMed: 1556098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DS2 / WFD11.
[2]	"The complete genome sequence of Haloferax volcanii DS2, a model archaeon." Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A. PLoS ONE 5:E9605-E9605(2010) [PubMed: 20333302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83531 Genomic DNA. Translation: AAA73174.1. CP001956 Genomic DNA. Translation: ADE02906.1.
PIR	A42149.
RefSeq	YP_003536599.1. NC_013967.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8926248.
KEGG	hvo:HVO_2583.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits.
KO	K00021.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_HALVD

Accession

Primary (citable) accession number: Q59468
Secondary accession number(s): D4GU18

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program