Reviewed, UniProtKB/Swiss-Prot Q59468 (HMDH_HALVO)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Synonyms:	hmg

Organism

Halobacterium volcanii (Haloferax volcanii)

Taxonomic identifier

2246 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Induction	Inhibited by mevinolin.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114460
Sites
	Active site	99	1	Charge relay system By similarity
	Active site	303	1	Charge relay system By similarity
	Active site	398	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59468-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7EB79919587BD724	FASTA	403	41,213
	10 20 30 40 50 60 MTDAASLADR VREGDLRLHE LEAHADADTA AEARRLLVES QSGASLDAVG NYGFPAEAAE 70 80 90 100 110 120 SAIENMVGSI QVPMGVAGPV SVDGGSVAGE KYLPLATTEG ALLASVNRGC SVINSAGGAT 130 140 150 160 170 180 ARVLKSGMTR APVFRVADVA EAEALVSWTR DNFAALKEAA EETTNHGELL DVTPYVVGNS 190 200 210 220 230 240 VYLRFRYDTK DAMGMNMATI ATEAVCGVVE AETAASLVAL SGNLCSDKKP AAINAVEGRG 250 260 270 280 290 300 RSVTADVRIP REVVEERLHT TPERGRELNT RKNLVGSAKA ASLGFNAHVA NVVAAMFLAT 310 320 330 340 350 360 GQDEAQVVEG ANAITTAEVQ DGDLYVSVSI ASLEVGTVGG GTKLPTQSEG LDILGVSGGG 370 380 390 400 DPAGSNADAL AECIAVGSLA GELSLLSALA SRHLSSAHAE LGR

References

[1]

"Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii." Lam W.L., Doolittle W.F. J. Biol. Chem. 267:5829-5834(1992) [PubMed: 1556098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WFD11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83531 Genomic DNA. Translation: AAA73174.1.
PIR	A42149.
3D structure databases
SMR	Q59468. Positions 7-394.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.34. 430.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_HALVO

Accession

Primary (citable) accession number: Q59468

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)