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Protein

Cadmium, zinc and cobalt-transporting ATPase

Gene

cadA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. This ion efflux may influence the activity of urease, which is essential for the survival of the bacterium in the gastric environment.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111Cadmium or zinc or cobaltPROSITE-ProRule annotation
Metal bindingi14 – 141Cadmium or zinc or cobaltPROSITE-ProRule annotation
Active sitei388 – 38814-aspartylphosphate intermediateBy similarity
Metal bindingi583 – 5831MagnesiumPROSITE-ProRule annotation
Metal bindingi587 – 5871MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cadmium-exporting ATPase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. zinc-exporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. metal ion transport Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP0791-MONOMER.

Protein family/group databases

TCDBi3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cadmium, zinc and cobalt-transporting ATPase (EC:3.6.3.3, EC:3.6.3.5)
Gene namesi
Name:cadA
Ordered Locus Names:HP_0791
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7272Cytoplasmic1 PublicationAdd
BLAST
Transmembranei73 – 9220Helical; Name=1Add
BLAST
Topological domaini93 – 10210Extracellular1 Publication
Transmembranei103 – 12422Helical; Name=2Add
BLAST
Topological domaini125 – 1317Cytoplasmic1 Publication
Transmembranei132 – 15120Helical; Name=3Add
BLAST
Topological domaini152 – 1543Extracellular1 Publication
Transmembranei155 – 17420Helical; Name=4Add
BLAST
Topological domaini175 – 308134Cytoplasmic1 PublicationAdd
BLAST
Transmembranei309 – 32719Helical; Name=5Add
BLAST
Topological domaini328 – 3325Extracellular1 Publication
Transmembranei333 – 35018Helical; Name=6Add
BLAST
Topological domaini351 – 635285Cytoplasmic1 PublicationAdd
BLAST
Transmembranei636 – 65722Helical; Name=7Add
BLAST
Topological domaini658 – 6658Extracellular1 Publication
Transmembranei666 – 68116Helical; Name=8Add
BLAST
Topological domaini682 – 6865Cytoplasmic1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Cadmium, zinc and cobalt-transporting ATPasePRO_0000046176Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ59465.

Interactioni

Protein-protein interaction databases

DIPiDIP-3082N.
IntActiQ59465. 3 interactions.
MINTiMINT-188226.
STRINGi85962.HP0791.

Structurei

3D structure databases

ProteinModelPortaliQ59465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6363HMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2217.
KOiK01534.
OMAiEKAMFFV.
OrthoDBiEOG6742RM.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEYHIHNLD CPDCASKLER DLNELDYVKK AQINFSTSKL FLDTSDFEKV
60 70 80 90 100
KAFIKQNEPH LSLSFKEATE KPLSFTPLII TIMVFLGAIL ILHLNPSPLI
110 120 130 140 150
EKAMFFVLAL VYLVSGKDVI LGAFRGLRKG QFFDENALML IATIAAFFVG
160 170 180 190 200
AYEESVSIMV FYSAGEFLQK LAVSRSKKSL KALVDVAPNL AYLKKGDELV
210 220 230 240 250
SVAPEDLRVN DIVVVKVGEK VPVDGVVVKG ESLLDERALS GESMPVNVSE
260 270 280 290 300
NSKVLGGSLN LKAVLEIQVE KMYKDSSIAK VVDLVQQATN EKSETEKFIT
310 320 330 340 350
KFSRYYTPSV LFIALMIAVL PPLFSMGSFD EWIYRGLVAL MVSCPCALVI
360 370 380 390 400
SVPLGYFGGV GAASRKGILM KGVHVLEVLT QAKSIAFDKT GTLTKGVFKV
410 420 430 440 450
TDIVPQNGHS KEEVLHYASC SQLLSTHPIA LSIQKACEEM LKDDKHQHDI
460 470 480 490 500
KNYEEVSGMG VKAQCHTDLI IAGNEKMLDQ FHIAHSPSKE NGTIVHVAFN
510 520 530 540 550
QTYVGYIVIS DEIKDDAIEC LRDLKVQGIE NFCILSGDRK SATESIAQTL
560 570 580 590 600
GCEYHASLLP EEKTSVFKTF KERYKAPAIF VGDGINDAPT LASADVGIGM
610 620 630 640 650
GKGSELSKQS ADIVITNDSL NSLVKVLAIA KKTKSIIWQN ILFALGIKAV
660 670 680
FIVLGLMGVA SLWEAVFGDV GVTLLALANS MRAMRA
Length:686
Mass (Da):75,012
Last modified:October 31, 1997 - v2
Checksum:i8777A49D80397E19
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241E → K in strain: 69A.
Natural varianti39 – 391K → R in strain: 69A.
Natural varianti69 – 691T → A in strain: 69A.
Natural varianti80 – 801I → V in strain: 69A.
Natural varianti83 – 831M → A in strain: 69A.
Natural varianti148 – 1481F → C in strain: 69A.
Natural varianti173 – 1742VS → IA in strain: 69A.
Natural varianti198 – 1981E → A in strain: 69A.
Natural varianti209 – 2091V → I in strain: 69A.
Natural varianti228 – 2281V → I in strain: 69A.
Natural varianti251 – 2511N → R in strain: 69A.
Natural varianti435 – 4351K → E in strain: 69A.
Natural varianti456 – 4561V → L in strain: 69A.
Natural varianti504 – 5041V → I in strain: 69A.
Natural varianti526 – 5261V → A in strain: 69A.
Natural varianti555 – 5551H → Y in strain: 69A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46864 Genomic DNA. Translation: AAA93043.1.
AE000511 Genomic DNA. Translation: AAD07839.1.
PIRiG64618.
RefSeqiNP_207584.1. NC_000915.1.
YP_006934705.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07839; AAD07839; HP_0791.
GeneIDi13869974.
899347.
KEGGiheo:C694_04055.
hpy:HP0791.
PATRICi20592869. VBIHelPyl33062_0823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46864 Genomic DNA. Translation: AAA93043.1.
AE000511 Genomic DNA. Translation: AAD07839.1.
PIRiG64618.
RefSeqiNP_207584.1. NC_000915.1.
YP_006934705.1. NC_018939.1.

3D structure databases

ProteinModelPortaliQ59465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3082N.
IntActiQ59465. 3 interactions.
MINTiMINT-188226.
STRINGi85962.HP0791.

Protein family/group databases

TCDBi3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PRIDEiQ59465.

Protocols and materials databases

DNASUi899347.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07839; AAD07839; HP_0791.
GeneIDi13869974.
899347.
KEGGiheo:C694_04055.
hpy:HP0791.
PATRICi20592869. VBIHelPyl33062_0823.

Phylogenomic databases

eggNOGiCOG2217.
KOiK01534.
OMAiEKAMFFV.
OrthoDBiEOG6742RM.

Enzyme and pathway databases

BioCyciHPY:HP0791-MONOMER.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and membrane topology of a P type ATPase from Helicobacter pylori."
    Melchers K., Weitzenegger T., Buhmann A., Steinhilber W., Sachs G., Schaefer K.P.
    J. Biol. Chem. 271:446-457(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 69A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  3. "Helicobacter pylori cadA encodes an essential Cd(II)-Zn(II)-Co(II) resistance factor influencing urease activity."
    Herrmann L., Schwan D., Garner R., Mobley H.L., Haas R., Schaefer K.P., Melchers K.
    Mol. Microbiol. 33:524-536(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
    Strain: 69A.
  4. "Membrane topology of CadA homologous P-type ATPase of Helicobacter pylori as determined by expression of phoA fusions in Escherichia coli and the positive inside rule."
    Melchers K., Schuhmacher A., Buhmann A., Weitzenegger T., Belin D., Grau S., Ehrmann M.
    Res. Microbiol. 150:507-520(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY.
    Strain: 69A.

Entry informationi

Entry nameiHMCT_HELPY
AccessioniPrimary (citable) accession number: Q59465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1997
Last modified: January 6, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.