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Q59465 (HMCT_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cadmium, zinc and cobalt-transporting ATPase

EC=3.6.3.3
EC=3.6.3.5
Gene names
Name:cadA
Ordered Locus Names:HP_0791
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. This ion efflux may influence the activity of urease, which is essential for the survival of the bacterium in the gastric environment.

Catalytic activity

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).

ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification]

Contains 1 HMA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 686686Cadmium, zinc and cobalt-transporting ATPase
PRO_0000046176

Regions

Topological domain1 – 7272Cytoplasmic Ref.3
Transmembrane73 – 9220Helical; Name=1
Topological domain93 – 10210Extracellular Ref.3
Transmembrane103 – 12422Helical; Name=2
Topological domain125 – 1317Cytoplasmic Ref.3
Transmembrane132 – 15120Helical; Name=3
Topological domain152 – 1543Extracellular Ref.3
Transmembrane155 – 17420Helical; Name=4
Topological domain175 – 308134Cytoplasmic Ref.3
Transmembrane309 – 32719Helical; Name=5
Topological domain328 – 3325Extracellular Ref.3
Transmembrane333 – 35018Helical; Name=6
Topological domain351 – 635285Cytoplasmic Ref.3
Transmembrane636 – 65722Helical; Name=7
Topological domain658 – 6658Extracellular Ref.3
Transmembrane666 – 68116Helical; Name=8
Topological domain682 – 6865Cytoplasmic Ref.3
Domain1 – 6363HMA

Sites

Active site38814-aspartylphosphate intermediate By similarity
Metal binding111Cadmium or zinc or cobalt Potential
Metal binding141Cadmium or zinc or cobalt Potential
Metal binding5831Magnesium By similarity
Metal binding5871Magnesium By similarity

Natural variations

Natural variant241E → K in strain: 69A.
Natural variant391K → R in strain: 69A.
Natural variant691T → A in strain: 69A.
Natural variant801I → V in strain: 69A.
Natural variant831M → A in strain: 69A.
Natural variant1481F → C in strain: 69A.
Natural variant173 – 1742VS → IA in strain: 69A.
Natural variant1981E → A in strain: 69A.
Natural variant2091V → I in strain: 69A.
Natural variant2281V → I in strain: 69A.
Natural variant2511N → R in strain: 69A.
Natural variant4351K → E in strain: 69A.
Natural variant4561V → L in strain: 69A.
Natural variant5041V → I in strain: 69A.
Natural variant5261V → A in strain: 69A.
Natural variant5551H → Y in strain: 69A.

Sequences

Sequence LengthMass (Da)Tools
Q59465 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 8777A49D80397E19

FASTA68675,012
        10         20         30         40         50         60 
MQEYHIHNLD CPDCASKLER DLNELDYVKK AQINFSTSKL FLDTSDFEKV KAFIKQNEPH 

        70         80         90        100        110        120 
LSLSFKEATE KPLSFTPLII TIMVFLGAIL ILHLNPSPLI EKAMFFVLAL VYLVSGKDVI 

       130        140        150        160        170        180 
LGAFRGLRKG QFFDENALML IATIAAFFVG AYEESVSIMV FYSAGEFLQK LAVSRSKKSL 

       190        200        210        220        230        240 
KALVDVAPNL AYLKKGDELV SVAPEDLRVN DIVVVKVGEK VPVDGVVVKG ESLLDERALS 

       250        260        270        280        290        300 
GESMPVNVSE NSKVLGGSLN LKAVLEIQVE KMYKDSSIAK VVDLVQQATN EKSETEKFIT 

       310        320        330        340        350        360 
KFSRYYTPSV LFIALMIAVL PPLFSMGSFD EWIYRGLVAL MVSCPCALVI SVPLGYFGGV 

       370        380        390        400        410        420 
GAASRKGILM KGVHVLEVLT QAKSIAFDKT GTLTKGVFKV TDIVPQNGHS KEEVLHYASC 

       430        440        450        460        470        480 
SQLLSTHPIA LSIQKACEEM LKDDKHQHDI KNYEEVSGMG VKAQCHTDLI IAGNEKMLDQ 

       490        500        510        520        530        540 
FHIAHSPSKE NGTIVHVAFN QTYVGYIVIS DEIKDDAIEC LRDLKVQGIE NFCILSGDRK 

       550        560        570        580        590        600 
SATESIAQTL GCEYHASLLP EEKTSVFKTF KERYKAPAIF VGDGINDAPT LASADVGIGM 

       610        620        630        640        650        660 
GKGSELSKQS ADIVITNDSL NSLVKVLAIA KKTKSIIWQN ILFALGIKAV FIVLGLMGVA 

       670        680 
SLWEAVFGDV GVTLLALANS MRAMRA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and membrane topology of a P type ATPase from Helicobacter pylori."
Melchers K., Weitzenegger T., Buhmann A., Steinhilber W., Sachs G., Schaefer K.P.
J. Biol. Chem. 271:446-457(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 69A.
[2]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
[3]"Helicobacter pylori cadA encodes an essential Cd(II)-Zn(II)-Co(II) resistance factor influencing urease activity."
Herrmann L., Schwan D., Garner R., Mobley H.L., Haas R., Schaefer K.P., Melchers K.
Mol. Microbiol. 33:524-536(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
Strain: 69A.
[4]"Membrane topology of CadA homologous P-type ATPase of Helicobacter pylori as determined by expression of phoA fusions in Escherichia coli and the positive inside rule."
Melchers K., Schuhmacher A., Buhmann A., Weitzenegger T., Belin D., Grau S., Ehrmann M.
Res. Microbiol. 150:507-520(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY.
Strain: 69A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L46864 Genomic DNA. Translation: AAA93043.1.
AE000511 Genomic DNA. Translation: AAD07839.1.
PIRG64618.
RefSeqNP_207584.1. NC_000915.1.
YP_006934705.1. NC_018939.1.

3D structure databases

ProteinModelPortalQ59465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3082N.
IntActQ59465. 3 interactions.
MINTMINT-188226.
STRING85962.HP0791.

Protein family/group databases

TCDB3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PRIDEQ59465.

Protocols and materials databases

DNASU899347.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07839; AAD07839; HP_0791.
GeneID13869974.
899347.
KEGGheo:C694_04055.
hpy:HP0791.
PATRIC20592869. VBIHelPyl33062_0823.

Phylogenomic databases

eggNOGCOG2217.
KOK01534.
OMALMVSCPC.
OrthoDBEOG6742RM.
ProtClustDBCLSK496215.

Enzyme and pathway databases

BioCycHPY:HP0791-MONOMER.

Family and domain databases

Gene3D2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR027256. Cation_transp_P-typ_ATPase_IB.
IPR001757. Cation_transp_P_typ_ATPase.
IPR027265. Di_cation_transp_P_ATPase.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
[Graphical view]
PANTHERPTHR24093:SF126. PTHR24093:SF126. 1 hit.
PfamPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00941. CDATPASE.
SUPFAMSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMCT_HELPY
AccessionPrimary (citable) accession number: Q59465
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names