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Protein

Cadmium, zinc and cobalt-transporting ATPase

Gene

cadA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. This ion efflux may influence the activity of urease, which is essential for the survival of the bacterium in the gastric environment.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Cadmium or zinc or cobaltPROSITE-ProRule annotation1
Metal bindingi14Cadmium or zinc or cobaltPROSITE-ProRule annotation1
Active sitei3884-aspartylphosphate intermediateBy similarity1
Metal bindingi583MagnesiumPROSITE-ProRule annotation1
Metal bindingi587MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • metal ion transport Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP0791-MONOMER.

Protein family/group databases

TCDBi3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cadmium, zinc and cobalt-transporting ATPase (EC:3.6.3.3, EC:3.6.3.5)
Gene namesi
Name:cadA
Ordered Locus Names:HP_0791
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 72Cytoplasmic1 PublicationAdd BLAST72
Transmembranei73 – 92Helical; Name=1Add BLAST20
Topological domaini93 – 102Extracellular1 Publication10
Transmembranei103 – 124Helical; Name=2Add BLAST22
Topological domaini125 – 131Cytoplasmic1 Publication7
Transmembranei132 – 151Helical; Name=3Add BLAST20
Topological domaini152 – 154Extracellular1 Publication3
Transmembranei155 – 174Helical; Name=4Add BLAST20
Topological domaini175 – 308Cytoplasmic1 PublicationAdd BLAST134
Transmembranei309 – 327Helical; Name=5Add BLAST19
Topological domaini328 – 332Extracellular1 Publication5
Transmembranei333 – 350Helical; Name=6Add BLAST18
Topological domaini351 – 635Cytoplasmic1 PublicationAdd BLAST285
Transmembranei636 – 657Helical; Name=7Add BLAST22
Topological domaini658 – 665Extracellular1 Publication8
Transmembranei666 – 681Helical; Name=8Add BLAST16
Topological domaini682 – 686Cytoplasmic1 Publication5

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000461761 – 686Cadmium, zinc and cobalt-transporting ATPaseAdd BLAST686

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ59465.

Interactioni

Protein-protein interaction databases

DIPiDIP-3082N.
IntActiQ59465. 3 interactors.
MINTiMINT-188226.
STRINGi85962.HP0791.

Structurei

3D structure databases

ProteinModelPortaliQ59465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 63HMAPROSITE-ProRule annotationAdd BLAST63

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C59. Bacteria.
COG2217. LUCA.
KOiK01534.
OMAiHQHDIKN.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEYHIHNLD CPDCASKLER DLNELDYVKK AQINFSTSKL FLDTSDFEKV
60 70 80 90 100
KAFIKQNEPH LSLSFKEATE KPLSFTPLII TIMVFLGAIL ILHLNPSPLI
110 120 130 140 150
EKAMFFVLAL VYLVSGKDVI LGAFRGLRKG QFFDENALML IATIAAFFVG
160 170 180 190 200
AYEESVSIMV FYSAGEFLQK LAVSRSKKSL KALVDVAPNL AYLKKGDELV
210 220 230 240 250
SVAPEDLRVN DIVVVKVGEK VPVDGVVVKG ESLLDERALS GESMPVNVSE
260 270 280 290 300
NSKVLGGSLN LKAVLEIQVE KMYKDSSIAK VVDLVQQATN EKSETEKFIT
310 320 330 340 350
KFSRYYTPSV LFIALMIAVL PPLFSMGSFD EWIYRGLVAL MVSCPCALVI
360 370 380 390 400
SVPLGYFGGV GAASRKGILM KGVHVLEVLT QAKSIAFDKT GTLTKGVFKV
410 420 430 440 450
TDIVPQNGHS KEEVLHYASC SQLLSTHPIA LSIQKACEEM LKDDKHQHDI
460 470 480 490 500
KNYEEVSGMG VKAQCHTDLI IAGNEKMLDQ FHIAHSPSKE NGTIVHVAFN
510 520 530 540 550
QTYVGYIVIS DEIKDDAIEC LRDLKVQGIE NFCILSGDRK SATESIAQTL
560 570 580 590 600
GCEYHASLLP EEKTSVFKTF KERYKAPAIF VGDGINDAPT LASADVGIGM
610 620 630 640 650
GKGSELSKQS ADIVITNDSL NSLVKVLAIA KKTKSIIWQN ILFALGIKAV
660 670 680
FIVLGLMGVA SLWEAVFGDV GVTLLALANS MRAMRA
Length:686
Mass (Da):75,012
Last modified:November 1, 1997 - v2
Checksum:i8777A49D80397E19
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti24E → K in strain: 69A. 1
Natural varianti39K → R in strain: 69A. 1
Natural varianti69T → A in strain: 69A. 1
Natural varianti80I → V in strain: 69A. 1
Natural varianti83M → A in strain: 69A. 1
Natural varianti148F → C in strain: 69A. 1
Natural varianti173 – 174VS → IA in strain: 69A. 2
Natural varianti198E → A in strain: 69A. 1
Natural varianti209V → I in strain: 69A. 1
Natural varianti228V → I in strain: 69A. 1
Natural varianti251N → R in strain: 69A. 1
Natural varianti435K → E in strain: 69A. 1
Natural varianti456V → L in strain: 69A. 1
Natural varianti504V → I in strain: 69A. 1
Natural varianti526V → A in strain: 69A. 1
Natural varianti555H → Y in strain: 69A. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46864 Genomic DNA. Translation: AAA93043.1.
AE000511 Genomic DNA. Translation: AAD07839.1.
PIRiG64618.
RefSeqiNP_207584.1. NC_000915.1.
WP_001158808.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07839; AAD07839; HP_0791.
GeneIDi899347.
KEGGiheo:C694_04055.
hpy:HP0791.
PATRICi20592869. VBIHelPyl33062_0823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46864 Genomic DNA. Translation: AAA93043.1.
AE000511 Genomic DNA. Translation: AAD07839.1.
PIRiG64618.
RefSeqiNP_207584.1. NC_000915.1.
WP_001158808.1. NC_018939.1.

3D structure databases

ProteinModelPortaliQ59465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3082N.
IntActiQ59465. 3 interactors.
MINTiMINT-188226.
STRINGi85962.HP0791.

Protein family/group databases

TCDBi3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiQ59465.

Protocols and materials databases

DNASUi899347.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07839; AAD07839; HP_0791.
GeneIDi899347.
KEGGiheo:C694_04055.
hpy:HP0791.
PATRICi20592869. VBIHelPyl33062_0823.

Phylogenomic databases

eggNOGiENOG4105C59. Bacteria.
COG2217. LUCA.
KOiK01534.
OMAiHQHDIKN.

Enzyme and pathway databases

BioCyciHPY:HP0791-MONOMER.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMCT_HELPY
AccessioniPrimary (citable) accession number: Q59465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.