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Q59465

- HMCT_HELPY

UniProt

Q59465 - HMCT_HELPY

Protein

Cadmium, zinc and cobalt-transporting ATPase

Gene

cadA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. This ion efflux may influence the activity of urease, which is essential for the survival of the bacterium in the gastric environment.

    Catalytic activityi

    ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
    ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111Cadmium or zinc or cobaltPROSITE-ProRule annotation
    Metal bindingi14 – 141Cadmium or zinc or cobaltPROSITE-ProRule annotation
    Active sitei388 – 38814-aspartylphosphate intermediateBy similarity
    Metal bindingi583 – 5831MagnesiumPROSITE-ProRule annotation
    Metal bindingi587 – 5871MagnesiumPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cadmium-exporting ATPase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. zinc-exporting ATPase activity Source: UniProtKB-EC

    GO - Biological processi

    1. metal ion transport Source: CACAO

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Cadmium, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHPY:HP0791-MONOMER.

    Protein family/group databases

    TCDBi3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cadmium, zinc and cobalt-transporting ATPase (EC:3.6.3.3, EC:3.6.3.5)
    Gene namesi
    Name:cadA
    Ordered Locus Names:HP_0791
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 686686Cadmium, zinc and cobalt-transporting ATPasePRO_0000046176Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ59465.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-3082N.
    IntActiQ59465. 3 interactions.
    MINTiMINT-188226.
    STRINGi85962.HP0791.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59465.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7272Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini93 – 10210Extracellular1 Publication
    Topological domaini125 – 1317Cytoplasmic1 Publication
    Topological domaini152 – 1543Extracellular1 Publication
    Topological domaini175 – 308134Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini328 – 3325Extracellular1 Publication
    Topological domaini351 – 635285Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini658 – 6658Extracellular1 Publication
    Topological domaini682 – 6865Cytoplasmic1 Publication

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei73 – 9220Helical; Name=1Add
    BLAST
    Transmembranei103 – 12422Helical; Name=2Add
    BLAST
    Transmembranei132 – 15120Helical; Name=3Add
    BLAST
    Transmembranei155 – 17420Helical; Name=4Add
    BLAST
    Transmembranei309 – 32719Helical; Name=5Add
    BLAST
    Transmembranei333 – 35018Helical; Name=6Add
    BLAST
    Transmembranei636 – 65722Helical; Name=7Add
    BLAST
    Transmembranei666 – 68116Helical; Name=8Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6363HMAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HMA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2217.
    KOiK01534.
    OMAiLMVSCPC.
    OrthoDBiEOG6742RM.

    Family and domain databases

    Gene3Di2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR027256. Cation_transp_P-typ_ATPase_IB.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR017969. Heavy-metal-associated_CS.
    IPR006121. HeavyMe-assoc_HMA.
    [Graphical view]
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    PF00403. HMA. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00941. CDATPASE.
    SUPFAMiSSF55008. SSF55008. 1 hit.
    SSF56784. SSF56784. 2 hits.
    TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
    TIGR01494. ATPase_P-type. 1 hit.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    PS01047. HMA_1. 1 hit.
    PS50846. HMA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59465-1 [UniParc]FASTAAdd to Basket

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    MQEYHIHNLD CPDCASKLER DLNELDYVKK AQINFSTSKL FLDTSDFEKV    50
    KAFIKQNEPH LSLSFKEATE KPLSFTPLII TIMVFLGAIL ILHLNPSPLI 100
    EKAMFFVLAL VYLVSGKDVI LGAFRGLRKG QFFDENALML IATIAAFFVG 150
    AYEESVSIMV FYSAGEFLQK LAVSRSKKSL KALVDVAPNL AYLKKGDELV 200
    SVAPEDLRVN DIVVVKVGEK VPVDGVVVKG ESLLDERALS GESMPVNVSE 250
    NSKVLGGSLN LKAVLEIQVE KMYKDSSIAK VVDLVQQATN EKSETEKFIT 300
    KFSRYYTPSV LFIALMIAVL PPLFSMGSFD EWIYRGLVAL MVSCPCALVI 350
    SVPLGYFGGV GAASRKGILM KGVHVLEVLT QAKSIAFDKT GTLTKGVFKV 400
    TDIVPQNGHS KEEVLHYASC SQLLSTHPIA LSIQKACEEM LKDDKHQHDI 450
    KNYEEVSGMG VKAQCHTDLI IAGNEKMLDQ FHIAHSPSKE NGTIVHVAFN 500
    QTYVGYIVIS DEIKDDAIEC LRDLKVQGIE NFCILSGDRK SATESIAQTL 550
    GCEYHASLLP EEKTSVFKTF KERYKAPAIF VGDGINDAPT LASADVGIGM 600
    GKGSELSKQS ADIVITNDSL NSLVKVLAIA KKTKSIIWQN ILFALGIKAV 650
    FIVLGLMGVA SLWEAVFGDV GVTLLALANS MRAMRA 686
    Length:686
    Mass (Da):75,012
    Last modified:November 1, 1997 - v2
    Checksum:i8777A49D80397E19
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241E → K in strain: 69A.
    Natural varianti39 – 391K → R in strain: 69A.
    Natural varianti69 – 691T → A in strain: 69A.
    Natural varianti80 – 801I → V in strain: 69A.
    Natural varianti83 – 831M → A in strain: 69A.
    Natural varianti148 – 1481F → C in strain: 69A.
    Natural varianti173 – 1742VS → IA in strain: 69A.
    Natural varianti198 – 1981E → A in strain: 69A.
    Natural varianti209 – 2091V → I in strain: 69A.
    Natural varianti228 – 2281V → I in strain: 69A.
    Natural varianti251 – 2511N → R in strain: 69A.
    Natural varianti435 – 4351K → E in strain: 69A.
    Natural varianti456 – 4561V → L in strain: 69A.
    Natural varianti504 – 5041V → I in strain: 69A.
    Natural varianti526 – 5261V → A in strain: 69A.
    Natural varianti555 – 5551H → Y in strain: 69A.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L46864 Genomic DNA. Translation: AAA93043.1.
    AE000511 Genomic DNA. Translation: AAD07839.1.
    PIRiG64618.
    RefSeqiNP_207584.1. NC_000915.1.
    YP_006934705.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07839; AAD07839; HP_0791.
    GeneIDi13869974.
    899347.
    KEGGiheo:C694_04055.
    hpy:HP0791.
    PATRICi20592869. VBIHelPyl33062_0823.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L46864 Genomic DNA. Translation: AAA93043.1 .
    AE000511 Genomic DNA. Translation: AAD07839.1 .
    PIRi G64618.
    RefSeqi NP_207584.1. NC_000915.1.
    YP_006934705.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali Q59465.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3082N.
    IntActi Q59465. 3 interactions.
    MINTi MINT-188226.
    STRINGi 85962.HP0791.

    Protein family/group databases

    TCDBi 3.A.3.6.3. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    PRIDEi Q59465.

    Protocols and materials databases

    DNASUi 899347.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07839 ; AAD07839 ; HP_0791 .
    GeneIDi 13869974.
    899347.
    KEGGi heo:C694_04055.
    hpy:HP0791.
    PATRICi 20592869. VBIHelPyl33062_0823.

    Phylogenomic databases

    eggNOGi COG2217.
    KOi K01534.
    OMAi LMVSCPC.
    OrthoDBi EOG6742RM.

    Enzyme and pathway databases

    BioCyci HPY:HP0791-MONOMER.

    Family and domain databases

    Gene3Di 2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProi IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR027256. Cation_transp_P-typ_ATPase_IB.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR017969. Heavy-metal-associated_CS.
    IPR006121. HeavyMe-assoc_HMA.
    [Graphical view ]
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    PF00403. HMA. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00941. CDATPASE.
    SUPFAMi SSF55008. SSF55008. 1 hit.
    SSF56784. SSF56784. 2 hits.
    TIGRFAMsi TIGR01525. ATPase-IB_hvy. 1 hit.
    TIGR01494. ATPase_P-type. 1 hit.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    PS01047. HMA_1. 1 hit.
    PS50846. HMA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and membrane topology of a P type ATPase from Helicobacter pylori."
      Melchers K., Weitzenegger T., Buhmann A., Steinhilber W., Sachs G., Schaefer K.P.
      J. Biol. Chem. 271:446-457(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 69A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    3. "Helicobacter pylori cadA encodes an essential Cd(II)-Zn(II)-Co(II) resistance factor influencing urease activity."
      Herrmann L., Schwan D., Garner R., Mobley H.L., Haas R., Schaefer K.P., Melchers K.
      Mol. Microbiol. 33:524-536(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
      Strain: 69A.
    4. "Membrane topology of CadA homologous P-type ATPase of Helicobacter pylori as determined by expression of phoA fusions in Escherichia coli and the positive inside rule."
      Melchers K., Schuhmacher A., Buhmann A., Weitzenegger T., Belin D., Grau S., Ehrmann M.
      Res. Microbiol. 150:507-520(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY.
      Strain: 69A.

    Entry informationi

    Entry nameiHMCT_HELPY
    AccessioniPrimary (citable) accession number: Q59465
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3