Q59447 (CYSK_FLAS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:

cysK

Organism

Flavobacterium sp. (strain K3-15 / DSM ID92-509)

Taxonomic identifier

268949 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Cysteine synthase		PRO_0000167091
Regions
	Region	176 – 180	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	72	1	Pyridoxal phosphate By similarity
	Binding site	263	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	42	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59447 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 5A1D8199718BF2BD	FASTA	307	33,340
	10 20 30 40 50 60 MKFQNALETI GNTPVVKINN LFNSDHEIWI KLEKSNPGGS IKDRIALAMI EDAEAKGLLN 70 80 90 100 110 120 KDSTIIEPTS GNTGIGLALV AAVKGYKLIL VMPESMSIER RKIMEAYGAE FVLTPREKGM 130 140 150 160 170 180 KGAIEKANEL AEETPNSWIP RQFDNPANVK IHVETTAQEI LQDFPEGLDY VITGVGTGGH 190 200 210 220 230 240 ITGIAKALKE KYPNLKVIAV EPELSPVLSG GSPAPHPLQG LGAGFVPSIL DITLLDGVIT 250 260 270 280 290 300 VGKDEAYEYA INAAKKEGLF CGSFHRSRLS RYRKTFTGNT AWSKNSLPLI TTPEKGIFLL RDSSKIL

References

[1]	"Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15." Mueller R., Kuttler E., Lanz C., Drewks C., Schmidt K. FEMS Microbiol. Lett. 136:305-308(1996) [PubMed: 8867384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50729 Genomic DNA. Translation: CAA90597.1.
PIR	S58299.
3D structure databases
ProteinModelPortal	Q59447.
SMR	Q59447. Positions 4-264.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_FLAS3

Accession

Primary (citable) accession number: Q59447

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program