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Protein

RNA polymerase sigma factor RpoS

Gene

KatF

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.SAAS annotation
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi300 – 31920H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Sigma factorUniRule annotationSAAS annotation

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoSUniRule annotation
Alternative name(s):
Sigma SUniRule annotation
Sigma-38UniRule annotation
Gene namesi
Name:KatFImported
Synonyms:rpoSUniRule annotation
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Interacts with the RNA polymerase core enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi469008.ECD_02591.

Structurei

3D structure databases

ProteinModelPortaliQ59409.
SMRiQ59409. Positions 101-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 14314RNA_pol_sigma70InterPro annotationAdd
BLAST
Domaini299 – 32527RNA_pol_sigma70InterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 10134Sigma-70 factor domain-1UniRule annotationAdd
BLAST
Regioni106 – 17671Sigma-70 factor domain-2UniRule annotationAdd
BLAST
Regioni186 – 26176Sigma-70 factor domain-3UniRule annotationAdd
BLAST
Regioni274 – 32754Sigma-70 factor domain-4UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi130 – 1334Interaction with polymerase core subunit RpoCUniRule annotation

Sequence similaritiesi

Belongs to the sigma-70 factor family.SAAS annotation
Belongs to the sigma-70 factor family. RpoS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00959. Sigma70_RpoS.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQGITGRS HLMSQNTLKV HDLNEDAEFD ENGVEVLDEK ALVEQEPSDN
60 70 80 90 100
DLAEEELLSQ GATQRVLDAT QLYLGEIGYS PLLTAEEEVY FARRALRGDV
110 120 130 140 150
ASRRRMIESN LRLVVKIARR YGNRGLALLD LIEEGNLGLI RAVEKFDPER
160 170 180 190 200
GFRFSTYATW WIRQTIERAI MNQTRTIRLP IHIVKELNVY LRTARELSHK
210 220 230 240 250
LDHEPSAEEI AEQLDKPVDD VSRMLRLNER ITSVDTPLGG DSEKALLDIL
260 270 280 290 300
ADEKENGPED TTQDDDMKQS IVKWLFELNA KQREVLARRF GLLGYEAATL
310 320 330 340 350
EDVGREIGLT RERVRQIQVE GLRRLREILQ TQGLNIEAPF ARNPANAGAE
360 370
YRSAVPRVSK HLSERPVSSE AGLFCAQ
Length:377
Mass (Da):42,930
Last modified:November 1, 1996 - v1
Checksum:i4B89C0670DBBBDEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14966 Genomic DNA. Translation: CAA78689.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14966 Genomic DNA. Translation: CAA78689.1.

3D structure databases

ProteinModelPortaliQ59409.
SMRiQ59409. Positions 101-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi469008.ECD_02591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00959. Sigma70_RpoS.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor."
    Mulvey M.R., Loewen P.C.
    Nucleic Acids Res. 17:9979-9991(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KatF 1137Imported.
  2. "DNA base sequence variability in katF (putative sigma factor) gene of Escherichia coli."
    Ivanova A., Renshaw M., Guntaka R.V., Eisenstark A.
    Nucleic Acids Res. 20:5479-5480(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KatF 1137Imported.
  3. Ivanova A.N.
    Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KatF 1137Imported.

Entry informationi

Entry nameiQ59409_ECOLX
AccessioniPrimary (citable) accession number: Q59409
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.