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Q59408 (DYR13_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase type A13

EC=1.5.1.3
Alternative name(s):
Dihydrofolate reductase type XIII
Short name=DHFRXIII
Gene names
Name:dfrA13
Synonyms:dfr13
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Dihydrofolate reductase type A13
PRO_0000186429

Regions

Domain7 – 162156DHFR

Sequences

Sequence LengthMass (Da)Tools
Q59408 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 656E9E214DDFDA9D

FASTA16517,925
        10         20         30         40         50         60 
MNPESVRIYL VAAMGANRVI GNGPDIPWKI PGEQKIFRRL TESKVVVMGR KTFESIGKPL 

        70         80         90        100        110        120 
PNRHTVVLSR QAGYSAPGCA VVSTLSHVSP STAEHGKELY VARGAEVYAL ALPHANGVFL 

       130        140        150        160 
SEVHQTFEGD AFFPVLNAAE FEVVSSETIQ GTITYTHSVY ARRNG 

« Hide

References

[1]"New gene cassettes for trimethoprim resistance, dfr13, and Streptomycin-spectinomycin resistance, aadA4, inserted on a class 1 integron."
Adrian P.V., Thomson C.J., Klugman K.P., Amyes S.G.
Antimicrob. Agents Chemother. 44:355-361(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RA33.2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50802 Genomic DNA. Translation: CAA90683.1.
PIRS60665.

3D structure databases

ProteinModelPortalQ59408.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ59408.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR13_ECOLX
AccessionPrimary (citable) accession number: Q59408
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways