Q59401 (Q59401_ENTCL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Class C beta-lactamase EMBL BAA07922.1 EC=3.5.2.6 EMBL BAA07922.1
Organism	Enterobacter cloacae EMBL BAA07922.1
Taxonomic identifier	550 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL BAA07922.1
Molecular function	Hydrolase EMBL BAA07922.1
Technical term	3D-structure PDB 1GA0 PDB 1ONH PDB 1Q2Q
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential EMBL BAA07922.1
	Chain	21 – 384	364	class C beta-lactamase EMBL BAA07922.1		PRO_5000139888

Sequences

	Sequence		Length	Mass (Da)
	Q59401 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B3BC13B02531372F	FASTA	384	41,612
	10 20 30 40 50 60 MMKKSLCCAL LLGISCSALA TPVSEKQLAE VVANTVTPLM KAQSVPGMAV AVIYQGKPHY 70 80 90 100 110 120 YTFGKADIAA NKPVTPQTLF ELGSISKTFT GVLGGDAIAR GEISLDDPVT RYWPQLTGKQ 130 140 150 160 170 180 WQGIRMLDLA TYTAGGLPLQ VPDEVTDNAS LLRFYQNWQP QWKPGTTRLY ANASIGLFGA 190 200 210 220 230 240 LAVKPSGMPY EQAMTTRVLK PLKLDHTWIN VPKAEEAHYA WGYRDGKAVR AVRVSPGMLD 250 260 270 280 290 300 AQAYGVKTNV QDMANWVMAN MAPENVADAS LKQGIALAQS RYWRIGSMYQ GLGWEMLNWP 310 320 330 340 350 360 VEANTVVEGS DSKVALAPLP VAEVNPPAPP VKASWVHKTG STGGFGSYVA FIPEKQIGIV 370 380 MLANTSYPNP ARVEAAYHIL EALQ

References

[1]	"Molecular evolution of a class C beta-lactamase extending its substrate specificity." Nukaga M., Haruta S., Tanimoto K., Kogure K., Taniguchi K., Tamaki M., Sawai T. J. Biol. Chem. 270:5729-5735(1995) [PubMed: 7890700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: GC1 EMBL BAA07922.1.
[2]	"Biochemical characterization of type A and type B beta-lactamase from Enterobacter cloacae." Then R.L., Charnas R.L., Kocher H.P., Manneberg M., Rothlisberger U., Stocker J. Rev. Infect. Dis. 10:714-720(1988) [PubMed: 3263683] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Inhibition of class C beta-lactamases: structure of a reaction intermediate with a cephem sulfone." Crichlow G.V., Nukaga M., Doppalapudi V.R., Buynak J.D., Knox J.R. Biochemistry 40:6233-6239(2001) [PubMed: 11371184] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 21-384.
[4]	"Inhibition of class A and class C beta-lactamases by penems: crystallographic structures of a novel 1,4-thiazepine intermediate." Nukaga M., Abe T., Venkatesan A.M., Mansour T.S., Bonomo R.A., Knox J.R. Biochemistry 42:13152-13159(2003) [PubMed: 14609325] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.38 ANGSTROMS) OF 21-384.
[5]	"Structure-activity relationship of 6-methylidene penems bearing tricyclic heterocycles as broad-spectrum beta-lactamase inhibitors: crystallographic structures show unexpected binding of 1,4-thiazepine intermediates." Venkatesan A.M., Gu Y., Dos Santos O., Abe T., Agarwal A., Yang Y., Petersen P.J., Weiss W.J., Mansour T.S., Nukaga M., Hujer A.M., Bonomo R.A., Knox J.R. J. Med. Chem. 47:6556-6568(2004) [PubMed: 15588091] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 22-384.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D44479 Genomic DNA. Translation: BAA07922.1.

PIR

B60908.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GA0	X-ray	1.60	A	21-384	[»]
1ONH	X-ray	1.38	A	21-384	[»]
1Q2Q	X-ray	1.40	A	22-384	[»]

ProteinModelPortal

Q59401.

SMR

Q59401. Positions 22-384.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]

Gene3D

G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q59401_ENTCL

Accession

Primary (citable) accession number: Q59401

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	April 5, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)