ID   DYR9_ECOLX              Reviewed;         177 AA.
AC   Q59397;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=Dihydrofolate reductase type 9;
DE            EC=1.5.1.3;
DE   AltName: Full=Dihydrofolate reductase type IX;
GN   Name=dhfrIX;
OS   Escherichia coli.
OG   Plasmid.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=3926;
RX   MEDLINE=92060953; PubMed=1659308;
RA   Jansson C., Skoeld O.;
RT   "Appearance of a new trimethoprim resistance gene, dhfrIX, in
RT   Escherichia coli from swine.";
RL   Antimicrob. Agents Chemother. 35:1891-1899(1991).
CC   -!- FUNCTION: Confers trimethoprim resistance.
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; X57730; CAA40897.1; -; Genomic_DNA.
DR   PIR; A49788; A49788.
DR   HSSP; P00379; 1DHI.
DR   BRENDA; 1.5.1.3; 246.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0031427; P:response to methotrexate; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   InterPro; IPR017925; Dihydrofolate_reductase_CS.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Methotrexate resistance; NADP;
KW   One-carbon metabolism; Oxidoreductase; Plasmid;
KW   Trimethoprim resistance.
FT   CHAIN         1    177       Dihydrofolate reductase type 9.
FT                                /FTId=PRO_0000186427.
FT   DOMAIN        3    167       DHFR.
SQ   SEQUENCE   177 AA;  19879 MW;  F478DBA8E745ABD0 CRC64;
     MASLNMIVAV NKTGGIGFEN QIPWHEPEDL KHFKAVTMNS VLIMGRKTFA SLPKVLPGRL
     HVVVSKTVPP TQNTDQVVYV STYQIAVRTA SLLVDKPEYS QIFVIGGKSA YENLAAYVDK
     LYLTRVQLNT QQDTELDLSL FKSWKLVSEV PTITENKTKL IFQIWINPNP ISEEPTC
//