Reviewed, UniProtKB/Swiss-Prot Q59397 (DYR9_ECOLX)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase type 9
    EC=1.5.1.3
Alternative name(s):
    Dihydrofolate reductase type IX

Gene names

Name:

dhfrIX

Encoded on

Plasmid

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Confers trimethoprim resistance.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Subunit structure	Homodimer By similarity.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Dihydrofolate reductase type 9		PRO_0000186427
Regions
	Domain	3 – 167	165	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q59397-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F478DBA8E745ABD0	FASTA	177	19,879
	10 20 30 40 50 60 MASLNMIVAV NKTGGIGFEN QIPWHEPEDL KHFKAVTMNS VLIMGRKTFA SLPKVLPGRL 70 80 90 100 110 120 HVVVSKTVPP TQNTDQVVYV STYQIAVRTA SLLVDKPEYS QIFVIGGKSA YENLAAYVDK 130 140 150 160 170 LYLTRVQLNT QQDTELDLSL FKSWKLVSEV PTITENKTKL IFQIWINPNP ISEEPTC

References

[1]	"Appearance of a new trimethoprim resistance gene, dhfrIX, in Escherichia coli from swine." Jansson C., Skoeld O. Antimicrob. Agents Chemother. 35:1891-1899(1991) [PubMed: 1659308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3926.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57730 Genomic DNA. Translation: CAA40897.1.
PIR	A49788.
3D structure databases
HSSP	HSSP built from PDB template 1DHI based on UniProtKB P00379.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR9_ECOLX

Accession

Primary (citable) accession number: Q59397

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)