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Q59395 (GUNW_PECSS) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 6

EC=3.2.1.4
Alternative name(s):
Cellulase V1
Endo-1,4-beta-glucanase V1
Endoglucanase V1
Gene names
Name:celV1
Ordered Locus Names:W5S_2582
OrganismPectobacterium sp. (strain SCC3193) [Complete proteome] [HAMAP]
Taxonomic identifier1166016 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 505474Endoglucanase 6
PRO_0000007859

Regions

Domain353 – 505153CBM3
Region32 – 334303Catalytic
Region335 – 35218Linker

Sites

Active site1681Proton donor By similarity
Active site2561Nucleophile By similarity

Experimental info

Sequence conflict4461Missing in CAA55823. Ref.1
Sequence conflict4821V → I in CAA55823. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q59395 [UniParc].

Last modified March 6, 2013. Version 2.
Checksum: 1E714E05E56453D3

FASTA50555,020
        10         20         30         40         50         60 
MWLRRKQIVR KLTLGVVTTM LGMSLSFSAL SATPVETHGQ LSIENGRLVD EQGKRVQLRG 

        70         80         90        100        110        120 
ISSNGLQWVG DYVNKDSMKW LRDDWGINVF RVAMYTAENG YIANPSLANK VKEAVAAAQG 

       130        140        150        160        170        180 
LGVYIIIDWH TLSDNDPNTY KAQAKIFFAE MAGLYGNSPN VIYEIANEPN GSVTWNGQIR 

       190        200        210        220        230        240 
PYALEVTDTI RSKDPDNLII VGSGTWSQDI HDAADNQLPD PNTLYALHFY AGTHGQFLRD 

       250        260        270        280        290        300 
RIDYAQSRGA AIFVSEWGTS DASGNGGPFL PESQTWIDFL NNRGISWVNW SLSDKSETSA 

       310        320        330        340        350        360 
ALVAGASKSG GWTEQNLSTS GKFVREQIRA GAGLSGGDTP TMPTEPTNPG NGTTGDIVLQ 

       370        380        390        400        410        420 
YRNVDNNPSD DAIRMAFNIK NTGSTPIKLS DLQVRYYFHD DGKPGANLFV DWANVGPNNI 

       430        440        450        460        470        480 
VTSTGTPAAS TDKANRYVLV TFASGAGSLQ PGAETGEVQV RIHAGDWSNV NETNDYSYGP 

       490        500 
NVTSYTNWDK ITVHDKGTLV WGTEP 

« Hide

References

« Hide 'large scale' references
[1]"Structure and regulation of the Erwinia carotovora subspecies carotovora SCC3193 cellulase gene celV1 and the role of cellulase in phytopathogenicity."
Mae A., Heikinheimo R., Palva E.T.
Mol. Gen. Genet. 247:17-26(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SCC3193.
[2]"Genome sequence of Pectobacterium sp. strain SCC3193."
Koskinen J.P., Laine P., Niemi O., Nykyri J., Harjunpaa H., Auvinen P., Paulin L., Pirhonen M., Palva T., Holm L.
J. Bacteriol. 194:6004-6004(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCC3193.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79241 Genomic DNA. Translation: CAA55823.1.
CP003415 Genomic DNA. Translation: AFI90670.1.
PIRS54744.
RefSeqYP_006283542.1. NC_017845.1.

3D structure databases

ProteinModelPortalQ59395.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID14760216.
KEGGpec:W5S_2582.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01179.

Family and domain databases

Gene3D2.60.40.710. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS51172. CBM3. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNW_PECSS
AccessionPrimary (citable) accession number: Q59395
Secondary accession number(s): K4FVJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 6, 2013
Last modified: October 16, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries