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Q59394

- GUNN_PECAT

UniProt

Q59394 - GUNN_PECAT

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Protein

Endoglucanase N

Gene

celN

Organism
Pectobacterium atrosepticum (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton donorBy similarity
Active sitei256 – 2561NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase N (EC:3.2.1.4)
Alternative name(s):
Cellulase N
Endo-1,4-beta-glucanase N
Gene namesi
Name:celN
OrganismiPectobacterium atrosepticum (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri29471 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 444413Endoglucanase NPRO_0000007857Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ59394.
SMRiQ59394. Positions 35-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini356 – 44489CBM3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2730.

Family and domain databases

Gene3Di2.60.40.710. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59394-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWMRRNQIVR KLTLGVVTTV LGMSLSFSAL SATPVETHGQ LSIENGRLVD
60 70 80 90 100
EQGKRVQLRG VSSHGLQWFG DYVNKDSMKW LRDDWGINVF RVAMYTAADG
110 120 130 140 150
YISNPSLANK VKEAVAAAQS LGVYIIIDWH ILSDNDPNIY KAQAKTFFAE
160 170 180 190 200
MAGLYGSSPN VIYEIANEPN GGVTWNGQIR PYALEVTDTI RSKDPDNLII
210 220 230 240 250
VGTGTWSQDI HDAADNQLPD PNTLYALHFY AGTHGQFLRD RIDYAQSRGA
260 270 280 290 300
AIFVSEWGTS DASGNGGPFL PESQTWIDFL NNRGVSWVNW SLTDKSEASA
310 320 330 340 350
ALAPGASKSG GWTEQNLSTS GKFVREQIRA GANLGGGDTP TTPTTPTEPT
360 370 380 390 400
NPGNGTTGDV VLQYRNVDNN PSDDAIRMAV NIKNTGSTPI KLSDLQVRYY
410 420 430 440
FHDDGKPGAN LFVDWANVGP NNIVTSTGTP AASTDKANRY VLVT
Length:444
Mass (Da):48,301
Last modified:November 1, 1996 - v1
Checksum:iFA7E4179004CBB43
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L39788 Genomic DNA. Translation: AAC37033.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L39788 Genomic DNA. Translation: AAC37033.1 .

3D structure databases

ProteinModelPortali Q59394.
SMRi Q59394. Positions 35-332.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2730.

Family and domain databases

Gene3Di 2.60.40.710. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SMARTi SM01067. CBM_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS51172. CBM3. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transplanting two unique beta-glucanase catalytic activities into one multienzyme, which forms glucose."
    Olsen O., Thomsen K.K., Weber J., Duus J.O., Svendsen I., Wegener C., von Wettstein D.
    Biotechnology (N.Y.) 14:71-76(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUNN_PECAT
AccessioniPrimary (citable) accession number: Q59394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3