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Q59334

- HEM2_CHLP8

UniProt

Q59334 - HEM2_CHLP8

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Protein
Delta-aminolevulinic acid dehydratase
Gene
hemB, Cpar_0732
Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Enzyme regulationi

Stimulated by magnesium, inhibited by zinc.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001Schiff-base intermediate with substrate2 Publications
Binding sitei210 – 2101Substrate 1
Binding sitei222 – 2221Substrate 1
Metal bindingi238 – 2381Magnesium
Active sitei253 – 2531Schiff-base intermediate with substrate2 Publications
Binding sitei279 – 2791Substrate 2
Binding sitei318 – 3181Substrate 2

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCPAR517417:GH95-761-MONOMER.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:Cpar_0732
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000008811: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Delta-aminolevulinic acid dehydratase
PRO_0000140499Add
BLAST

Interactioni

Subunit structurei

Homooctamer.1 Publication

Protein-protein interaction databases

STRINGi517417.Cpar_0732.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 164
Helixi20 – 267
Helixi33 – 353
Beta strandi36 – 4712
Beta strandi49 – 524
Beta strandi55 – 628
Helixi63 – 7513
Beta strandi80 – 856
Helixi95 – 984
Helixi103 – 11412
Beta strandi118 – 1247
Turni127 – 1293
Beta strandi135 – 1417
Helixi145 – 16218
Beta strandi165 – 1695
Helixi176 – 18611
Beta strandi192 – 20110
Helixi209 – 2124
Turni223 – 2253
Helixi233 – 24513
Beta strandi248 – 2547
Helixi256 – 2583
Helixi259 – 26911
Beta strandi273 – 2775
Helixi279 – 29012
Helixi296 – 31015
Beta strandi313 – 3175
Helixi320 – 3278

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W1ZX-ray2.60A/B1-328[»]
2C1HX-ray2.60A/B1-328[»]
ProteinModelPortaliQ59334.
SMRiQ59334. Positions 10-328.

Miscellaneous databases

EvolutionaryTraceiQ59334.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OMAiEVLCKMA.
OrthoDBiEOG6VXFCB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59334-1 [UniParc]FASTAAdd to Basket

« Hide

MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE    50
EVSSMPGSFR FTIDRAVEEC KELYDLGIQG IDLFGIPEQK TEDGSEAYND 100
NGILQQAIRA IKKAVPELCI MTDVALDPFT PFGHDGLVKD GIILNDETVE 150
VLQKMAVSHA EAGADFVSPS DMMDGRIGAI REALDETDHS DVGILSYAAK 200
YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTEEAMKEVE LDIVEGADIV 250
MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA KGWIDEDRVM 300
MESLLCMKRA GADIIFTYYA KEAAKKLR 328
Length:328
Mass (Da):36,395
Last modified:November 1, 1996 - v1
Checksum:iD31B234328ABBE7B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38348 Genomic DNA. Translation: AAC43975.1.
CP001099 Genomic DNA. Translation: ACF11151.1.
RefSeqiYP_001998351.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF11151; ACF11151; Cpar_0732.
GeneIDi6419660.
KEGGicpc:Cpar_0732.
PATRICi21364722. VBIChlPar72705_0731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38348 Genomic DNA. Translation: AAC43975.1 .
CP001099 Genomic DNA. Translation: ACF11151.1 .
RefSeqi YP_001998351.1. NC_011027.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W1Z X-ray 2.60 A/B 1-328 [» ]
2C1H X-ray 2.60 A/B 1-328 [» ]
ProteinModelPortali Q59334.
SMRi Q59334. Positions 10-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 517417.Cpar_0732.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF11151 ; ACF11151 ; Cpar_0732 .
GeneIDi 6419660.
KEGGi cpc:Cpar_0732.
PATRICi 21364722. VBIChlPar72705_0731.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OMAi EVLCKMA.
OrthoDBi EOG6VXFCB.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
BioCyci CPAR517417:GH95-761-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q59334.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the Chlorobium vibrioforme hemB gene and characterization of its encoded enzyme, porphobilinogen synthase."
    Rhie G.-E., Avissar Y.J., Beale S.I.
    J. Biol. Chem. 271:8176-8182(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIB 8327.
  3. "The X-ray structure of the plant like 5-aminolaevulinic acid dehydratase from Chlorobium vibrioforme complexed with the inhibitor laevulinic acid at 2.6 A resolution."
    Coates L., Beaven G., Erskine P.T., Beale S.I., Avissar Y.J., Gill R., Mohammed F., Wood S.P., Shoolingin-Jordan P., Cooper J.B.
    J. Mol. Biol. 342:563-570(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND LAEVULINIC ACID, ACTIVE SITE, SUBUNIT, ABSENCE OF CATALYTIC METAL COFACTOR.
  4. "Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor."
    Coates L., Beaven G., Erskine P.T., Beale S.I., Wood S.P., Shoolingin-Jordan P.M., Cooper J.B.
    Acta Crystallogr. D 61:1594-1598(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND 4,7-DIOXOSEBACIC ACID, ACTIVE SITE.

Entry informationi

Entry nameiHEM2_CHLP8
AccessioniPrimary (citable) accession number: Q59334
Secondary accession number(s): B3QMJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not seem to have a metal requirement for activity.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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