Reviewed, UniProtKB/Swiss-Prot Q59334 (HEM2_CHLP8)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	Cpar_0732

Organism

Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]

Taxonomic identifier

517417 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Enzyme regulation	Stimulated by magnesium, inhibited by zinc.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Miscellaneous	Does not seem to have a metal requirement for activity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 328

328

Delta-aminolevulinic acid dehydratase

PRO_0000140499

Sites

Active site

253

1

By similarity

Secondary structure

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328

Helix Strand Turn

Details...

Helix

13 – 16

4

Helix

20 – 26

7

Helix

33 – 35

3

Beta strand

36 – 47

12

Beta strand

49 – 52

4

Beta strand

55 – 62

8

Helix

63 – 75

13

Beta strand

80 – 85

6

Helix

95 – 98

4

Helix

103 – 114

12

Beta strand

118 – 124

7

Turn

127 – 129

3

Beta strand

135 – 141

7

Helix

145 – 162

18

Beta strand

165 – 169

5

Helix

176 – 186

11

Beta strand

192 – 201

10

Helix

209 – 212

4

Turn

223 – 225

3

Helix

233 – 245

13

Beta strand

248 – 254

7

Helix

256 – 258

3

Helix

259 – 269

11

Beta strand

273 – 277

5

Helix

279 – 290

12

Helix

296 – 310

15

Beta strand

313 – 317

5

Helix

320 – 327

8

Sequences

	Sequence		Length	Mass (Da)
	Q59334-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D31B234328ABBE7B	FASTA	328	36,395
	10 20 30 40 50 60 MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE EVSSMPGSFR 70 80 90 100 110 120 FTIDRAVEEC KELYDLGIQG IDLFGIPEQK TEDGSEAYND NGILQQAIRA IKKAVPELCI 130 140 150 160 170 180 MTDVALDPFT PFGHDGLVKD GIILNDETVE VLQKMAVSHA EAGADFVSPS DMMDGRIGAI 190 200 210 220 230 240 REALDETDHS DVGILSYAAK YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTEEAMKEVE 250 260 270 280 290 300 LDIVEGADIV MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA KGWIDEDRVM 310 320 MESLLCMKRA GADIIFTYYA KEAAKKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the Chlorobium vibrioforme hemB gene and characterization of its encoded enzyme, porphobilinogen synthase." Rhie G.-E., Avissar Y.J., Beale S.I. J. Biol. Chem. 271:8176-8182(1996) [PubMed: 8626508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Chlorobaculum parvum NCIB 8327." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U38348 Genomic DNA. Translation: AAC43975.1.
CP001099 Genomic DNA. Translation: ACF11151.1.

RefSeq

YP_001998351.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W1Z	X-ray	2.60	A/B	1-328	[»]
2C1H	X-ray	2.60	A/B	1-328	[»]

ModBase

Search...

Genome annotation databases

GeneID

6419660.

GenomeReviews

Gene locus Cpar_0732 in contig CP001099_GR.

KEGG

cpc:Cpar_0732.

Organism-specific databases

CMR

Search...

Phylogenomic databases

OMA

PELGVIT.

Enzyme and pathway databases

BRENDA

4.2.1.24. 190276.

Family and domain databases

InterPro

IPR001731. 4pyrrol_synth_porphobiln_synth.
IPR013785. Aldolase_TIM.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR11458. AlaD_dehydratase. 1 hit.

Pfam

PF00490. ALAD. 1 hit.
[Graphical view]

PIRSF

PIRSF001415. Porphbilin_synth. 1 hit.

PRINTS

PR00144. DALDHYDRTASE.

ProDom

PD002304. AlaD_dehydratase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HEM2_CHLP8

Accession

Primary (citable) accession number: Q59334
Secondary accession number(s): B3QMJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)