Cellulose 1,4-beta-cellobiosidase - Clostridium thermocellum

Preferred order of sections

Names and origin

Protein names

Submitted name:
Cellulose 1,4-beta-cellobiosidase EMBL CAA56918.1
EC=3.2.1.91 EMBL CAA56918.1

Gene names

Name:

cbhA EMBL CAA56918.1

Organism

Clostridium thermocellum EMBL CAA56918.1

Taxonomic identifier

1515 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	1230 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1RQ5 Metal-binding PDB 1RQ5
Molecular function	Glycosidase EMBL CAA56918.1 Hydrolase
Technical term	3D-structure PDB 1RQ5
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: InterPro cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

421

1

Calcium 1 PDB 1RQ5

Metal binding

428

1

Calcium 1; via carbonyl oxygen PDB 1RQ5

Metal binding

431

1

Calcium 1 PDB 1RQ5

Metal binding

433

1

Calcium 1; via carbonyl oxygen PDB 1RQ5

Metal binding

435

1

Calcium 1 PDB 1RQ5

Metal binding

438

1

Calcium 1 PDB 1RQ5

Metal binding

557

1

Calcium 2 PDB 1RQ5

Metal binding

559

1

Calcium 2; via carbonyl oxygen PDB 1RQ5

Metal binding

562

1

Calcium 2 PDB 1RQ5

Metal binding

563

1

Calcium 2 PDB 1RQ5

Metal binding

617

1

Calcium 2; via carbonyl oxygen PDB 1RQ5

Sequences

Sequence

Length

Mass (Da)

Tools

Q59325 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A398D9814B5D6A0E
Show »

FASTA

1,230

138,078

        10         20         30         40         50         60 
MKFRRSICTA VLLAVLLTLL VPTSVFALED NSSTLPPYKN DLLYERTFDE GLCYPWHTCE 

        70         80         90        100        110        120 
DSGGKCSFDV VDVPGQPGNK AFAVTVLDKG QNRWRVQMRH RGLTLEQGHT YRVRLKIWAD 

       130        140        150        160        170        180 
ASCKVYIKIG QMAEPYAEYW NNKWSPYTLT AGKVLEIDET FVMDKPTDDT CEFTFHLGGE 

       190        200        210        220        230        240 
LAATPPYTVY LDDVSLYDPE YTKPVEYILP QPDVRVNQVG YLPEGKKVAT VVCNSTQPVK 

       250        260        270        280        290        300 
WQLKNAAGVV VLEGYTEPKG LDKDSQDYVH WLDFSDFATE GIGYYFELPT VNSPTNYSHP 

       310        320        330        340        350        360 
FDIRKDIYTQ MKYDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGIEPN KGDTNVPTWP 

       370        380        390        400        410        420 
QDDEYAGIPQ KNYTKDVTGG WYDAGDHGKY VVNGGIAVWT LMNMYERAKI RGLDNWGPYR 

       430        440        450        460        470        480 
DGGMNIPEQN NGYPDILDEA RWEIEFFKKM QVTEKEDPSI AGMVHHKIHD FRWTALGMLP 

       490        500        510        520        530        540 
HEDPQPRYLR PVSTAATLNF AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY 

       550        560        570        580        590        600 
AEYTPGSGGP GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 

       610        620        630        640        650        660 
GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPATDIQ KARNNIAKAA DRWLENIEEQ 

       670        680        690        700        710        720 
GYRLPIKRAE DERAGYPWGS NSLHFEPDDL VMGYAYDFTG DSNISMECLT GISYLLGRNA 

       730        740        750        760        770        780 
MDQSYVTGYG ERPLQNPHDR FWTPQTSKRF PAPPPGIISG RPNSRFEDPT INAAVKKDTP 

       790        800        810        820        830        840 
PQKCFIDHTD SWSTNEITVN WNAPFAWVTA YLDEQYTDSE TDKVTIDSPV AGERFEAGKD 

       850        860        870        880        890        900 
INIRTVKSKT PVSKVEFYNG DTLISSDTTA PYTAKITGAA VGAYNLKAVA VLSDGRRIES 

       910        920        930        940        950        960 
PVTPVLVKVI VKPTVKLTAP KSNVVAYGNE FLKITATASD SDGKISRVDF LVDGEVIGSD 

       970        980        990       1000       1010       1020 
REAPYEYEWK AVEGNHEISV IAYDDDDAAS TPDSVKIFVK QARDVKVQYL CENTQTSTQE 

      1030       1040       1050       1060       1070       1080 
IKGKFNIVNT GNRDYSLKDI VLRYYFTKEH NSQLQFICYY TPIGSGNLIP SFGGSGDEHY 

      1090       1100       1110       1120       1130       1140 
LQLEFKDVKL PAGGQTGEIQ FVIRYADNSF HDQSNDYSFD PTIKAFQDYG KVTLYKNGEL 

      1150       1160       1170       1180       1190       1200 
VWGTPPGGTE PEEPEEPEEP EEPAIVYGDC NDDGKVNSTD VAVMKRYLKK ENVNINLDNA 

      1210       1220       1230 
DVNADGKVNS TDFSILKRYV MKNIEELPYR

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References

[1]	"Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA." Zverlov V.V., Velikodvorskaya G.V., Schwarz W.H., Bronnenmeier K., Kellermann J., Staudenbauer W.L. J. Bacteriol. 180:3091-3099(1998) [PubMed: 9620957] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: F7 EMBL CAA56918.1.
[2]	"Structural basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum." Schubot F.D., Kataeva I.A., Chang J., Shah A.K., Ljungdahl L.G., Rose J.P., Wang B.C. Biochemistry 43:1163-1170(2004) [PubMed: 14756552] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 208-818 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X80993 Genomic DNA. Translation: CAA56918.1.

PIR

S47466.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RQ5	X-ray	2.40	A	208-818	[»]

ProteinModelPortal

Q59325.

SMR

Q59325. Positions 1166-1230.

ModBase

Search...

Protein family/group databases

CAZy

CBM3. Carbohydrate-Binding Module Family 3.
CBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.2.1.91. 97464.

Family and domain databases

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.710. CBD_3. 1 hit.
G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF00942. CBM_3. 1 hit.
PF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]

SMART

SM01067. CBM_3. 1 hit.
[Graphical view]

SUPFAM

SSF49384. Cellul_bind. 1 hit.
SSF63446. Cellulos_enz_dockerin_1. 1 hit.
SSF49785. Gal_bind_like. 1 hit.
SSF48208. Glyco_trans_6hp. 1 hit.
SSF81296. Ig_E-set. 1 hit.

PROSITE

PS51172. CBM3. 1 hit.
PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q59325_CLOTM

Accession

Primary (citable) accession number: Q59325

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)