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Protein

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase

Gene

bphD

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).UniRule annotation

Catalytic activityi

2,6-dioxo-6-phenylhexa-3-enoate + H2O = benzoate + 2-oxopent-4-enoate.UniRule annotation

Pathwayi: biphenyl degradation

This protein is involved in step 4 of the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Biphenyl dioxygenase subunit beta (bphE), Biphenyl dioxygenase subunit alpha (bphA)
  2. Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (bphB)
  3. no protein annotated in this organism
  4. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)
This subpathway is part of the pathway biphenyl degradation, which is itself part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl, the pathway biphenyl degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511SubstrateUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Sitei112 – 1121Transition state stabilizerUniRule annotation
Binding sitei180 – 1801Substrate; via carbonyl oxygenUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation
Active sitei265 – 2651Proton acceptorUniRule annotation
Binding sitei266 – 2661SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BRENDAi3.7.1.8. 1590.
UniPathwayiUPA00155; UER00253.

Protein family/group databases

ESTHERicomte-bphD. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolaseUniRule annotation (EC:3.7.1.8UniRule annotation)
Short name:
HOPDA hydrolaseUniRule annotation
Alternative name(s):
2,6-dioxo-6-phenylhexa-3-enoate hydrolaseUniRule annotation
Gene namesi
Name:bphDUniRule annotation
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2862862-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolasePRO_0000373811Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ59324.
SMRiQ59324. Positions 2-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 432Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. BphD family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01688. Biphenyl_BphD. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR017727. HOPD_hydrolase_BphD.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03343. biphenyl_bphD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELTEGNTS KFAKISEKDL SDFLIHYNEA GEGEAVIMLH GGGPGAGGWS
60 70 80 90 100
NYYRNIGPFV DAGYRVILKD SPGFNKSDVV VMDEQRGLVN ARAVKGLMDA
110 120 130 140 150
LGIERAHLVG NSMGGATALN FAIEYPERLG KMILMGPGGL GASHFAPMPM
160 170 180 190 200
EGIKLLFKLY AEPSYETLRQ MIQVFLYDQT NITEELLQGR WEAIQRNPEH
210 220 230 240 250
LKNFLVSAQR APLSSWDVSP RLGEIKAKTL VTWGRDDRFV PLDHGLKLVW
260 270 280
GIGDARLHVF SQCGHWAQWE KADEFNRLAI DFLRQR
Length:286
Mass (Da):31,951
Last modified:November 1, 1996 - v1
Checksum:iD1A1A7F3D59439B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34338 Genomic DNA. Translation: AAA56853.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34338 Genomic DNA. Translation: AAA56853.1.

3D structure databases

ProteinModelPortaliQ59324.
SMRiQ59324. Positions 2-285.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERicomte-bphD. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00155; UER00253.
BRENDAi3.7.1.8. 1590.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01688. Biphenyl_BphD. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR017727. HOPD_hydrolase_BphD.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03343. biphenyl_bphD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBPHD_COMTE
AccessioniPrimary (citable) accession number: Q59324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.