Exo-alpha-sialidase precursor - Clostridium perfringens

Preferred order of sections

Names and origin

Protein names

Submitted name:
Exo-alpha-sialidase EMBL CAA60796.1
EC=3.2.1.18 EMBL CAA60796.1

Gene names

Name:

nanH EMBL CAA60796.1

Organism

Clostridium perfringens EMBL CAA60796.1

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL CAA60796.1
Ligand	Calcium PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7 Metal-binding PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7
Molecular function	Glycosidase EMBL CAA60796.1 Hydrolase
Technical term	3D-structure PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 41

41

Potential EMBL CAA60796.1

Chain

42 – 694

653

Potential EMBL CAA60796.1

PRO_5000146477

Sites

Metal binding

296

1

Calcium PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7

Metal binding

298

1

Calcium PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7

Metal binding

319

1

Calcium PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7

Metal binding

320

1

Calcium; via carbonyl oxygen PDB 2BF6 PDB 2VK5 PDB 2VK6 PDB 2VK7

Sequences

Sequence

Length

Mass (Da)

Tools

Q59310 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 22D23E25A0A8DC72
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FASTA

694

77,327

        10         20         30         40         50         60 
MNYKGITLIL TAAMVISGGN YVLVKGSTLD SGKNNSGYEI KVNNSENLSS LGEYKDINLE 

        70         80         90        100        110        120 
SSNASNITYD LEKYKNLDEG TIVVRFNSKD SKIQSLLGIS NSKTKNGYFN FYVTNSRVGF 

       130        140        150        160        170        180 
ELRNQKNEGN TQSGTENLVH MYKDVALNDG DNTVALKIEK NKGYKLFLNG KIIKEVKDTN 

       190        200        210        220        230        240 
TKFLNNIENL DSAFIGKTNR YGQSNEYNFK GNIGFMNIYN EPLGDDYLLS KTGETKAKEE 

       250        260        270        280        290        300 
VLVEGAVKTE PVDLFHPGFL NSSNYRIPAL FKTKEGTLIA SIDARRHGGA DAPNNDIDTA 

       310        320        330        340        350        360 
VRRSEDGGKT WDEGQIIMDY PDKSSVIDTT LIQDDETGRI FLLVTHFPSK YGFWNAGLGS 

       370        380        390        400        410        420 
GFKNIDGKEY LCLYDSSGKE FTVRENVVYD KDGNKTEYTT NALGDLFKNG TKIDNINSST 

       430        440        450        460        470        480 
APLKAKGTSY INLVYSDDDG KTWSEPQNIN FQVKKDWMKF LGIAPGRGIQ IKNGEHKGRI 

       490        500        510        520        530        540 
VVPVYYTNEK GKQSSAVIYS DDSGKNWTIG ESPNDNRKLE NGKIINSKTL SDDAPQLTEC 

       550        560        570        580        590        600 
QVVEMPNGQL KLFMRNLSGY LNIATSFDGG ATWDETVEKD TNVLEPYCQL SVINYSQKVD 

       610        620        630        640        650        660 
GKDAVIFSNP NARSRSNGTV RIGLINQVGT YENGEPKYEF DWKYNKLVKP GYYAYSCLTE 

       670        680        690 
LSNGNIGLLY EGTPSEEMSY IEMNLKYLES GANK

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References

[1]	"Gene structure of the 'large' sialidase isoenzyme from Clostridium perfringens A99 and its relationship with other clostridial nanH proteins." Traving C., Schauer R., Roggentin P. Glycoconj. J. 11:141-151(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: A99 EMBL CAA60796.1.
[2]	Schauer R. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A99 EMBL CAA60796.1.
[3]	"The structure of Clostridium perfringens NanI sialidase and its catalytic intermediates." Newstead S.L., Potter J.A., Wilson J.C., Xu G., Chien C.H., Watts A.G., Withers S.G., Taylor G.L. J. Biol. Chem. 283:9080-9088(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 243-691 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X87369 Genomic DNA. Translation: CAA60796.1.

PIR

I40866.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BF6	X-ray	0.97	A	243-691	[»]
2VK5	X-ray	0.97	A	243-694	[»]
2VK6	X-ray	1.50	A	243-694	[»]
2VK7	X-ray	1.20	A/B	243-694	[»]

ProteinModelPortal

Q59310.

SMR

Q59310. Positions 42-236, 243-690.

ModBase

Search...

Protein family/group databases

CAZy

CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.120.10.10. 2 hits.
2.60.120.200. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]

PANTHER

PTHR10628. PTHR10628. 1 hit.

Pfam

PF02973. Sialidase. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.
SSF50939. Sialidase. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q59310.

Entry information

Entry name

Q59310_CLOPF

Accession

Primary (citable) accession number: Q59310

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)