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Q59294 (HEM4_CLOJO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Porphyrin biosynthesis protein HemD

Including the following 2 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Uroporphyrinogen-III synthase
    Short name=UROS
    EC=4.2.1.75
    Alternative name(s):
    Hydroxymethylbilane hydrolyase [cyclizing]
    Uroporphyrinogen-III cosynthase
Gene names
Name:hemD
OrganismClostridium josui
Taxonomic identifier1499 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses.

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

Sequence similarities

Belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Porphyrin biosynthesis protein HemD
PRO_0000150383

Regions

Region1 – 248248Uroporphyrinogen-III C-methyltransferase
Region249 – 504256Uroporphyrinogen-III synthase

Sequences

Sequence LengthMass (Da)Tools
Q59294 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 86FB0DACEFD641D7

FASTA50455,211
        10         20         30         40         50         60 
MEHGFVALVG AGPGDKGLIT IRGAELLSQA DVVVYDRLVS QEIIKMIPTT AEKIDVGKEN 

        70         80         90        100        110        120 
KFHPVKQEEI NHILLEKSLE GKKVIRLKGG DPFVFGRGGE ELELLYENNI PFEVVPGVTS 

       130        140        150        160        170        180 
AVAALCYGGI PATHRDFCSS LHIITGHARE GGQLSIPFHE LKELNGTIVF LMGDSSLSYL 

       190        200        210        220        230        240 
MNGLINAGME KDMPAAIVEN GTRPNQRKLV ATVGTLEQKA LEMEIKSPAI IAVGKVCSLS 

       250        260        270        280        290        300 
EKFSWFMKKP LFGTKILVTR PKESSGTLVE KLRQLGAEPV EYPCIEVVPI PQNEKLYHAC 

       310        320        330        340        350        360 
ENIREYGWIL LTSKNGIQIF FDYLNSKGLD ARVLANTKIG TVGSQTAKAL KEVGLISDFT 

       370        380        390        400        410        420 
PEIFDGRHLA LGIAERVGEN EKVLICDAAI ASDDIVNILR SNNIKFDRVP LYNTNYINEN 

       430        440        450        460        470        480 
SNKVKKSIVH GELKYITFTS ASTVEGFIAS MKDIPLESLT AVCIGNKTAE AAKKYNLRYV 

       490        500 
VAEKSTIDSM IDKLLEIGGG NIYD 

« Hide

References

[1]"Cloning and sequencing of some genes responsible for porphyrin biosynthesis from the anaerobic bacterium Clostridium josui."
Fujino E., Fujino T., Karita S., Sakka K., Ohmiya K.
J. Bacteriol. 177:5169-5175(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FERM P-9684.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D28503 Genomic DNA. Translation: BAA05862.1.
PIRI40811.

3D structure databases

ProteinModelPortalQ59294.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00148; UER00211.
UPA00262; UER00211.

Family and domain databases

Gene3D3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProIPR000878. 4pyrrol_Mease.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMSSF53790. SSF53790. 1 hit.
SSF69618. SSF69618. 1 hit.
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM4_CLOJO
AccessionPrimary (citable) accession number: Q59294
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 16, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways