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Q59294

- HEM4_CLOJO

UniProt

Q59294 - HEM4_CLOJO

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Protein

Porphyrin biosynthesis protein HemD

Gene

hemD

Organism
Clostridium josui
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.
Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC
  3. uroporphyrinogen-III synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. siroheme biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphyrin biosynthesis protein HemD
Including the following 2 domains:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
OrganismiClostridium josui
Taxonomic identifieri1499 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Porphyrin biosynthesis protein HemDPRO_0000150383Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ59294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 248248Uroporphyrinogen-III C-methyltransferaseAdd
BLAST
Regioni249 – 504256Uroporphyrinogen-III synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin methyltransferase family.Curated
In the C-terminal section; belongs to the uroporphyrinogen-III synthase family.Curated

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
SSF69618. SSF69618. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59294-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEHGFVALVG AGPGDKGLIT IRGAELLSQA DVVVYDRLVS QEIIKMIPTT
60 70 80 90 100
AEKIDVGKEN KFHPVKQEEI NHILLEKSLE GKKVIRLKGG DPFVFGRGGE
110 120 130 140 150
ELELLYENNI PFEVVPGVTS AVAALCYGGI PATHRDFCSS LHIITGHARE
160 170 180 190 200
GGQLSIPFHE LKELNGTIVF LMGDSSLSYL MNGLINAGME KDMPAAIVEN
210 220 230 240 250
GTRPNQRKLV ATVGTLEQKA LEMEIKSPAI IAVGKVCSLS EKFSWFMKKP
260 270 280 290 300
LFGTKILVTR PKESSGTLVE KLRQLGAEPV EYPCIEVVPI PQNEKLYHAC
310 320 330 340 350
ENIREYGWIL LTSKNGIQIF FDYLNSKGLD ARVLANTKIG TVGSQTAKAL
360 370 380 390 400
KEVGLISDFT PEIFDGRHLA LGIAERVGEN EKVLICDAAI ASDDIVNILR
410 420 430 440 450
SNNIKFDRVP LYNTNYINEN SNKVKKSIVH GELKYITFTS ASTVEGFIAS
460 470 480 490 500
MKDIPLESLT AVCIGNKTAE AAKKYNLRYV VAEKSTIDSM IDKLLEIGGG

NIYD
Length:504
Mass (Da):55,211
Last modified:November 1, 1997 - v1
Checksum:i86FB0DACEFD641D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28503 Genomic DNA. Translation: BAA05862.1.
PIRiI40811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28503 Genomic DNA. Translation: BAA05862.1 .
PIRi I40811.

3D structure databases

ProteinModelPortali Q59294.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00262 ; UER00211 .

Family and domain databases

Gene3Di 3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProi IPR000878. 4pyrrol_Mease.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF02602. HEM4. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53790. SSF53790. 1 hit.
SSF69618. SSF69618. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of some genes responsible for porphyrin biosynthesis from the anaerobic bacterium Clostridium josui."
    Fujino E., Fujino T., Karita S., Sakka K., Ohmiya K.
    J. Bacteriol. 177:5169-5175(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: FERM P-9684.

Entry informationi

Entry nameiHEM4_CLOJO
AccessioniPrimary (citable) accession number: Q59294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 1, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3