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Q59294

- HEM4_CLOJO

UniProt

Q59294 - HEM4_CLOJO

Protein

Porphyrin biosynthesis protein HemD

Gene

hemD

Organism
Clostridium josui
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.
    Hydroxymethylbilane = uroporphyrinogen III + H2O.

    Pathwayi

    GO - Molecular functioni

    1. precorrin-2 dehydrogenase activity Source: InterPro
    2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC
    3. uroporphyrinogen-III synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. siroheme biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase, Methyltransferase, Transferase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00148; UER00211.
    UPA00262; UER00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Porphyrin biosynthesis protein HemD
    Including the following 2 domains:
    Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
    Short name:
    Urogen III methylase
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name:
    UROM
    Uroporphyrinogen-III synthase (EC:4.2.1.75)
    Short name:
    UROS
    Alternative name(s):
    Hydroxymethylbilane hydrolyase [cyclizing]
    Uroporphyrinogen-III cosynthase
    Gene namesi
    Name:hemD
    OrganismiClostridium josui
    Taxonomic identifieri1499 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Porphyrin biosynthesis protein HemDPRO_0000150383Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ59294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 248248Uroporphyrinogen-III C-methyltransferaseAdd
    BLAST
    Regioni249 – 504256Uroporphyrinogen-III synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the precorrin methyltransferase family.Curated
    In the C-terminal section; belongs to the uroporphyrinogen-III synthase family.Curated

    Family and domain databases

    Gene3Di3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR003754. 4pyrrol_synth_uPrphyn_synth.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF02602. HEM4. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53790. SSF53790. 1 hit.
    SSF69618. SSF69618. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59294-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEHGFVALVG AGPGDKGLIT IRGAELLSQA DVVVYDRLVS QEIIKMIPTT    50
    AEKIDVGKEN KFHPVKQEEI NHILLEKSLE GKKVIRLKGG DPFVFGRGGE 100
    ELELLYENNI PFEVVPGVTS AVAALCYGGI PATHRDFCSS LHIITGHARE 150
    GGQLSIPFHE LKELNGTIVF LMGDSSLSYL MNGLINAGME KDMPAAIVEN 200
    GTRPNQRKLV ATVGTLEQKA LEMEIKSPAI IAVGKVCSLS EKFSWFMKKP 250
    LFGTKILVTR PKESSGTLVE KLRQLGAEPV EYPCIEVVPI PQNEKLYHAC 300
    ENIREYGWIL LTSKNGIQIF FDYLNSKGLD ARVLANTKIG TVGSQTAKAL 350
    KEVGLISDFT PEIFDGRHLA LGIAERVGEN EKVLICDAAI ASDDIVNILR 400
    SNNIKFDRVP LYNTNYINEN SNKVKKSIVH GELKYITFTS ASTVEGFIAS 450
    MKDIPLESLT AVCIGNKTAE AAKKYNLRYV VAEKSTIDSM IDKLLEIGGG 500
    NIYD 504
    Length:504
    Mass (Da):55,211
    Last modified:November 1, 1997 - v1
    Checksum:i86FB0DACEFD641D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28503 Genomic DNA. Translation: BAA05862.1.
    PIRiI40811.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28503 Genomic DNA. Translation: BAA05862.1 .
    PIRi I40811.

    3D structure databases

    ProteinModelPortali Q59294.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00262 ; UER00211 .

    Family and domain databases

    Gene3Di 3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR003754. 4pyrrol_synth_uPrphyn_synth.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF02602. HEM4. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53790. SSF53790. 1 hit.
    SSF69618. SSF69618. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of some genes responsible for porphyrin biosynthesis from the anaerobic bacterium Clostridium josui."
      Fujino E., Fujino T., Karita S., Sakka K., Ohmiya K.
      J. Bacteriol. 177:5169-5175(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: FERM P-9684.

    Entry informationi

    Entry nameiHEM4_CLOJO
    AccessioniPrimary (citable) accession number: Q59294
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3