Q59294 (HEM4_CLOJO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porphyrin biosynthesis protein HemD

Including the following 2 domains:

1. Uroporphyrinogen-III C-methyltransferase
   Short name=Urogen III methylase
   EC=2.1.1.107
   Alternative name(s):
   SUMT
   Uroporphyrinogen III methylase
   Short name=UROM
2. Uroporphyrinogen-III synthase
   Short name=UROS
   EC=4.2.1.75
   Alternative name(s):
   Hydroxymethylbilane hydrolyase [cyclizing]
   Uroporphyrinogen-III cosynthase

Gene names

Name:

hemD

Organism

Clostridium josui

Taxonomic identifier

1499 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses.
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. Hydroxymethylbilane = uroporphyrinogen III + H2O.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway siroheme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: InterPro uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: EC uroporphyrinogen-III synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Porphyrin biosynthesis protein HemD		PRO_0000150383
Regions
	Region	1 – 248	248	Uroporphyrinogen-III C-methyltransferase
	Region	249 – 504	256	Uroporphyrinogen-III synthase

Sequences

	Sequence		Length	Mass (Da)
	Q59294 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 86FB0DACEFD641D7	FASTA	504	55,211
	10 20 30 40 50 60 MEHGFVALVG AGPGDKGLIT IRGAELLSQA DVVVYDRLVS QEIIKMIPTT AEKIDVGKEN 70 80 90 100 110 120 KFHPVKQEEI NHILLEKSLE GKKVIRLKGG DPFVFGRGGE ELELLYENNI PFEVVPGVTS 130 140 150 160 170 180 AVAALCYGGI PATHRDFCSS LHIITGHARE GGQLSIPFHE LKELNGTIVF LMGDSSLSYL 190 200 210 220 230 240 MNGLINAGME KDMPAAIVEN GTRPNQRKLV ATVGTLEQKA LEMEIKSPAI IAVGKVCSLS 250 260 270 280 290 300 EKFSWFMKKP LFGTKILVTR PKESSGTLVE KLRQLGAEPV EYPCIEVVPI PQNEKLYHAC 310 320 330 340 350 360 ENIREYGWIL LTSKNGIQIF FDYLNSKGLD ARVLANTKIG TVGSQTAKAL KEVGLISDFT 370 380 390 400 410 420 PEIFDGRHLA LGIAERVGEN EKVLICDAAI ASDDIVNILR SNNIKFDRVP LYNTNYINEN 430 440 450 460 470 480 SNKVKKSIVH GELKYITFTS ASTVEGFIAS MKDIPLESLT AVCIGNKTAE AAKKYNLRYV 490 500 VAEKSTIDSM IDKLLEIGGG NIYD

References

[1]

"Cloning and sequencing of some genes responsible for porphyrin biosynthesis from the anaerobic bacterium Clostridium josui." Fujino E., Fujino T., Karita S., Sakka K., Ohmiya K. J. Bacteriol. 177:5169-5175(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FERM P-9684.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28503 Genomic DNA. Translation: BAA05862.1.
PIR	I40811.
3D structure databases
ProteinModelPortal	Q59294.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00148; UER00211. UPA00262; UER00211.
Family and domain databases
Gene3D	3.30.950.10. 1 hit. 3.40.1010.10. 1 hit.
InterPro	IPR000878. 4pyrrol_Mease. IPR003754. 4pyrrol_synth_uPrphyn_synth. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA/CysG_C. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Pfam	PF02602. HEM4. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
SUPFAM	SSF53790. Cor/por_Metransf. 1 hit. SSF69618. HEM4_synth. 1 hit.
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM4_CLOJO

Accession

Primary (citable) accession number: Q59294

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program