Q59290 (Q59290_CLOJO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-1,4-endoglucanase EMBL BAA12826.2
EC=3.2.1.4 EMBL BAA12826.2

Gene names

Name:

celA EMBL BAA12826.2

Organism

Clostridium josui EMBL BAA12826.2

Taxonomic identifier

1499 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	930 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI Metal-binding PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
Molecular function	Glycosidase EMBL BAA12826.2 Hydrolase
Technical term	3D-structure PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	580	1	Calcium; via carbonyl oxygen PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
	Metal binding	582	1	Calcium PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
	Metal binding	609	1	Calcium PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
	Metal binding	610	1	Calcium; via carbonyl oxygen PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI
	Metal binding	747	1	Calcium PDB 3ACF PDB 3ACG PDB 3ACH PDB 3ACI

Sequences

	Sequence		Length	Mass (Da)
	Q59290 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 6EBCAA364541F902	FASTA	930	101,643
	10 20 30 40 50 60 MFRNTNKRIL AFVIVVAMLM YFIPTMTFAV EEDSSHLITN QAKKPSTAGA LQLLNKNGVK 70 80 90 100 110 120 TLCDKDGNPI QLRGMSTHGL QWFPEIINNN AFAALSKDWG SNVIRLAMYV AEGGYSKDPE 130 140 150 160 170 180 IIKKRVIDGI DLAIANDMYV IVDWHVLTPG DPNADVYKGA MDFFKEISQK YPNNPHIIYE 190 200 210 220 230 240 LANEPSPNDP GVTNDAAGWA KVKSYAEPII KILRDSGNKN LIIVGSPNWS QRPDLAAENP 250 260 270 280 290 300 INDNNTAYSF HFYSGTHKTS TDSTDRGNIM SNARYALEHG VAVFCSEWGT SEASGNNGPY 310 320 330 340 350 360 LKEADEWLEF LNANNISWIN WSLTNKNETS GSFIPFISGK SDATNLNPGD DQVWSLKELS 370 380 390 400 410 420 VSGEYARARI KGIKYEPIER SEKEEFTTNV WDFNDGTTQG FGINGDSPIK ADSITLANEK 430 440 450 460 470 480 NALKITGLNN SNDLTEGNYW ANVRLSADGT SNKPNIFGAE KLTMDVITAA PATVSIAAIP 490 500 510 520 530 540 QSSTHGWANP TRAIAVKPAD FVKQEDGTYK AVLTITPADS PNFDSIAKDS KDSTMTNIIL 550 560 570 580 590 600 FVGADTDVIS LDNITVSGNR AVVEAPVEHA PIGKATLPST FEDSTRQGWA WDATSGVQSA 610 620 630 640 650 660 LTIKDANESK AISWEVKYPE VKPVDGWASA PRIMLGNVNT TRGNNKYLTF DFYLKPTQAS 670 680 690 700 710 720 KGSLTISLAF APPSLGFWAQ ATGDVNIPLS SLSKMKKTTD GLYHFQVKYD LDKINDGKVL 730 740 750 760 770 780 TANTVLRDIT IVVADGNSDF AGTMYLDNIR FENDSKTELN NSITMLVSKG IINNADVKKI 790 800 810 820 830 840 NFNSSISRGE FLMWIVKTLD LNAKFSSNFS DVNKKGSYYN SVGIAKALGI TSGVGNNKFN 850 860 870 880 890 900 PNKAISREDM LVLTYKALKI VNKNLAKGNA NDLKQFTDAS KVSKNAVESV ATIVKNGFYS 910 920 930 GDAKKLNPKA SVAKSEAALM LYKIYSSLHK

References

[1]	"Revised sequence of celA gene encoding endoglucanase (EG)-1 from Clostridium josui." Fujino T., Fujino E., Karita S., Ohmiya K. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Purification and properties of an endo-1,4-beta-glucanase translated from a Clostridium josui gene in Escherichia coli." Fujino T., Sasaki T., Ohmiya K., Shimizu S. Appl. Environ. Microbiol. 56:1175-1178(1990) [PubMed: 2187404] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module." Tsukimoto K., Takada R., Araki Y., Suzuki K., Karita S., Wakagi T., Shoun H., Watanabe T., Fushinobu S. FEBS Lett. 584:1205-1211(2010) [PubMed: 20159017] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 560-752 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D85526 Genomic DNA. Translation: BAA12826.2.

PIR

A60156.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ACF	X-ray	1.60	A	560-752	[»]
3ACG	X-ray	1.50	A	560-752	[»]
3ACH	X-ray	1.40	A	560-752	[»]
3ACI	X-ray	1.60	A	560-752	[»]

ProteinModelPortal

Q59290.

SMR

Q59290. Positions 43-382, 384-554.

ModBase

Search...

Protein family/group databases

CAZy

CBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. S-layer_homology_dom.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q59290_CLOJO

Accession

Primary (citable) accession number: Q59290

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	July 25, 2006
Last modified:	September 21, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)