Q59280 (ARGJ_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate N-acetyltransferase
EC=2.3.1.35

Alternative name(s):
Ornithine acetyltransferase
Ornithine transacetylase
Short name=OATase

Cleaved into the following 2 chains:

1. Glutamate N-acetyltransferase alpha chain
2. Glutamate N-acetyltransferase beta chain

Gene names

Name:	argJ
Ordered Locus Names:	Cgl1395, cg1581

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the ArgJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Autocatalytic cleavage
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: InterPro glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Glutamate N-acetyltransferase alpha chain By similarity		PRO_0000002161
	Chain	183 – 388	206	Glutamate N-acetyltransferase beta chain By similarity		PRO_0000002162
Sites
	Site	182 – 183	2	Cleavage; by autolysis By similarity
Experimental info
	Sequence conflict	135 – 136	2	GA → AP in CAA60097. Ref.1
	Sequence conflict	135 – 136	2	GA → AP in AAC24813. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q59280 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 031643D9D320A433	FASTA	388	39,714
	10 20 30 40 50 60 MAEKGITAPK GFVASATTAG IKASGNPDMA LVVNQGPEFS AAAVFTRNRV FAAPVKVSRE 70 80 90 100 110 120 NVADGQIRAV LYNAGNANAC NGLQGEKDAR ESVSHLAQNL GLEDSDIGVC STGLIGELLP 130 140 150 160 170 180 MDKLNAGIDQ LTAEGALGDN GAAAAKAIMT TDTVDKETVV FADGWTVGGM GKGVGMMAPS 190 200 210 220 230 240 LATMLVCLTT DASVTQEMAQ IALANATAVT FDTLDIDGST STNDTVFLLA SGASGITPTQ 250 260 270 280 290 300 DELNDAVYAA CSDIAAKLQA DAEGVTKRVA VTVVGTTNNE QAINAARTVA RDNLFKCAMF 310 320 330 340 350 360 GSDPNWGRVL AAVGMADADM EPEKISVFFN GQAVCLDSTG APGAREVDLS GADIDVRIDL 370 380 GTSGEGQATV RTTDLSFSYV EINSAYSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway." Sakanyan V., Petrosyan P., Lecocq M., Boyen A., Legrain C., Demarez M.N., Hallet J.-N., Glansdorff N. Microbiology 142:99-108(1996) [PubMed: 8581175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum." Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86157 Genomic DNA. Translation: CAA60097.1. AF049897 Genomic DNA. Translation: AAC24813.1. BA000036 Genomic DNA. Translation: BAB98788.1. BX927152 Genomic DNA. Translation: CAF21406.1.
RefSeq	NP_600614.1. NC_003450.3. YP_225682.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q59280.
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019371. 3343818.
GenomeReviews	Gene locus Cgl1395 in contig BA000036_GR. Gene locus cg1581 in contig BX927147_GR.
KEGG	cgb:cg1581. cgl:NCgl1341.
PATRIC	21494829. VBICorGlu203724_1364.
Phylogenomic databases
HOGENOM	HBG284202.
OMA	GRDPNWG.
PhylomeDB	Q59280.
ProtClustDB	PRK05388.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1581-MONOMER.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
KO	K00620.
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_CORGL

Accession

Primary (citable) accession number: Q59280

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2002
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program