Q59279 (ARGC_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-gamma-glutamyl-phosphate reductase
Short name=AGPR
EC=1.2.1.38

Alternative name(s):
N-acetyl-glutamate semialdehyde dehydrogenase
Short name=NAGSA dehydrogenase

Gene names

Name:	argC
Ordered Locus Names:	Cgl1394, cg1580

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH. HAMAP MF_00150
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. HAMAP MF_00150
Subcellular location	Cytoplasm Probable HAMAP MF_00150.
Sequence similarities	Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.
Sequence caution	The sequence CAF21405.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	N-acetyl-gamma-glutamyl-phosphate reductase HAMAP MF_00150		PRO_0000112400
Sites
	Active site	151	1	By similarity
Experimental info
	Sequence conflict	14	1	A → T in AAB62245. Ref.1
	Sequence conflict	14	1	A → T in AAC24812. Ref.2
	Sequence conflict	21	1	L → I in AAB62245. Ref.1
	Sequence conflict	21	1	L → I in AAC24812. Ref.2
	Sequence conflict	25	1	H → Q in AAB62245. Ref.1
	Sequence conflict	25	1	H → Q in AAC24812. Ref.2
	Sequence conflict	102	1	C → R in AAB62245. Ref.1
	Sequence conflict	102	1	C → R in AAC24812. Ref.2
	Sequence conflict	277	1	L → S in AAB62245. Ref.1
	Sequence conflict	277	1	L → S in AAC24812. Ref.2
	Sequence conflict	341	1	P → A in AAB62245. Ref.1
	Sequence conflict	341	1	P → A in AAC24812. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q59279 [UniParc]. Last modified July 11, 2002. Version 3. Checksum: 44A73448E7BF4864	FASTA	347	35,888
	10 20 30 40 50 60 MTIKVAIAGA SGYAGGEILR LLLGHPAYAS GELEIGALTA ASTAGSTLGE LMPHIPQLAD 70 80 90 100 110 120 RVIQDTTAET LAGHDVVFLG LPHGFSAEIA LQLGPDVTVI DCAADFRLQN AADWEKFYGS 130 140 150 160 170 180 EHQGTWPYGI PEMPGHREAL RGAKRVAVPG CFPTGATLAL LPAVQAGLIE PDVSVVSITG 190 200 210 220 230 240 VSGAGKKASV ALLGSETMGS LKAYNTSGKH RHTPEIAQNL GEVSDKPVKV SFTPVLAPLP 250 260 270 280 290 300 RGILTTATAP LKEGVTAEQA RAVYEEFYAQ ETFVHVLPEG AQPQTQAVLG SNMCHVQVEI 310 320 330 340 DEEAGKVLVT SAIDNLTKGT AGAAVQCMNL SVGFDEAAGL PQVGVAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Chun J.Y., Lee E.J., Cheon C.I., Min K.H., Lee M.-S. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum." Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]	"Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway." Sakanyan V., Petrosyan P., Lecocq M., Boyen A., Legrain C., Demarez M.N., Hallet J.-N., Glansdorff N. Microbiology 142:99-108(1996) [PubMed: 8581175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 243-347. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005242 Genomic DNA. Translation: AAB62245.1. AF049897 Genomic DNA. Translation: AAC24812.1. BA000036 Genomic DNA. Translation: BAB98787.1. BX927152 Genomic DNA. Translation: CAF21405.1. Different initiation. X86157 Genomic DNA. Translation: CAA60096.1.
RefSeq	NP_600613.1. NC_003450.3. YP_225681.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q59279.
SMR	Q59279. Positions 4-347.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019370. 3344983.
GenomeReviews	Gene locus Cgl1394 in contig BA000036_GR. Gene locus cg1580 in contig BX927147_GR.
KEGG	cgb:cg1580. cgl:NCgl1340.
PATRIC	21494827. VBICorGlu203724_1363.
Phylogenomic databases
HOGENOM	HBG294213.
OMA	VCRIAVH.
PhylomeDB	Q59279.
ProtClustDB	PRK00436.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1580-MONOMER.
Family and domain databases
HAMAP	MF_00150. ArgC_type1. [Tree]
InterPro	IPR023013. AGPR_AS. IPR000706. AGPR_type-1. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_dimer_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00145.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01850. ArgC. 1 hit.
PROSITE	PS01224. ARGC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGC_CORGL

Accession

Primary (citable) accession number: Q59279
Secondary accession number(s): O32353

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2002
Last modified:	January 25, 2012
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program