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Q59266 (MALQ_CLOBU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-alpha-glucanotransferase

EC=2.4.1.25
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme
Gene names
Name:malQ
OrganismClostridium butyricum
Taxonomic identifier1492 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a disproportionation reaction in which single or multiple glucose units from oligosaccharides are transferred to the 4-hydroxyl group of acceptor sugars. Glucose, maltose and maltotriose can act as acceptor, whereas of the three only maltotriose can act as donor.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the disproportionating enzyme family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4-alpha-glucanotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

transferase activity, transferring glycosyl groups

Inferred from direct assay Ref.1. Source: CACAO

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4874874-alpha-glucanotransferase
PRO_0000170124

Sequences

Sequence LengthMass (Da)Tools
Q59266 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FA9FBE3E74771DBE

FASTA48757,178
        10         20         30         40         50         60 
MHISSLPGKY GIGTFGRSAY EFCDFLEKAG QKYWQILPLG QTSYGDSPYQ SFSAFAGNPY 

        70         80         90        100        110        120 
FIDLDILNEK NLLDKDDYEE KNFGDNKEMI NYGLIFNEKM KVLRKAYMNF NSKDDESFAK 

       130        140        150        160        170        180 
FIEDEKKWLD DYSLFMALKY KFNFISWNSW NKDIKLRKNE EIEKYKDELK EDVNYWKFLQ 

       190        200        210        220        230        240 
YEFFSQWKNL KDYANKKNIK IIGDIPIYIA QDSSDVWSNP DIFLLNKETL EPLKVSGCPP 

       250        260        270        280        290        300 
DAFSETGQLW GNPIYDWGYL EKTNFEWWVD RIKSSLKLYD ILRIDHFRGF EAYWSVDYGE 

       310        320        330        340        350        360 
KTAQNGKWIK GPEMKLFNVI KEKIGDIEII AEDLGYLTEE TLEFKKRTGF PGMKIIQFAF 

       370        380        390        400        410        420 
GGDSSNPYLP HNYEKNCVAY TGTHDNDTVR GWFEVTGSKE EKEKAVEYFK LTEEEGYNWG 

       430        440        450        460        470        480 
VIRGVWSSVA NTSIGVMQDF LNLGNEARIN KPSTLASNWS WRAKDNVFTN ELANKIYRLT 


RIYGRCE 

« Hide

References

[1]"Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-alpha-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli."
Goda S.K., Eissa O., Akhtar M., Minton N.P.
Microbiology 143:3287-3294(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: ATCC 19398 / DSM 10702 / JCM 1391 / NCIMB 7423.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37874 Genomic DNA. Translation: AAB84229.1.

3D structure databases

ProteinModelPortalQ59266.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH77. Glycoside Hydrolase Family 77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.25. 1465.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR003385. Glyco_hydro_77.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02446. Glyco_hydro_77. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR00217. malQ. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMALQ_CLOBU
AccessionPrimary (citable) accession number: Q59266
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families