Q59266 (MALQ_CLOBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-alpha-glucanotransferase
EC=2.4.1.25

Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme

Gene names

Name:

malQ

Organism

Clostridium butyricum

Taxonomic identifier

1492 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes a disproportionation reaction in which single or multiple glucose units from oligosaccharides are transferred to the 4-hydroxyl group of acceptor sugars. Glucose, maltose and maltotriose can act as acceptor, whereas of the three only maltotriose can act as donor.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the disproportionating enzyme family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	4-alpha-glucanotransferase		PRO_0000170124

Sequences

	Sequence		Length	Mass (Da)
	Q59266 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FA9FBE3E74771DBE	FASTA	487	57,178
	10 20 30 40 50 60 MHISSLPGKY GIGTFGRSAY EFCDFLEKAG QKYWQILPLG QTSYGDSPYQ SFSAFAGNPY 70 80 90 100 110 120 FIDLDILNEK NLLDKDDYEE KNFGDNKEMI NYGLIFNEKM KVLRKAYMNF NSKDDESFAK 130 140 150 160 170 180 FIEDEKKWLD DYSLFMALKY KFNFISWNSW NKDIKLRKNE EIEKYKDELK EDVNYWKFLQ 190 200 210 220 230 240 YEFFSQWKNL KDYANKKNIK IIGDIPIYIA QDSSDVWSNP DIFLLNKETL EPLKVSGCPP 250 260 270 280 290 300 DAFSETGQLW GNPIYDWGYL EKTNFEWWVD RIKSSLKLYD ILRIDHFRGF EAYWSVDYGE 310 320 330 340 350 360 KTAQNGKWIK GPEMKLFNVI KEKIGDIEII AEDLGYLTEE TLEFKKRTGF PGMKIIQFAF 370 380 390 400 410 420 GGDSSNPYLP HNYEKNCVAY TGTHDNDTVR GWFEVTGSKE EKEKAVEYFK LTEEEGYNWG 430 440 450 460 470 480 VIRGVWSSVA NTSIGVMQDF LNLGNEARIN KPSTLASNWS WRAKDNVFTN ELANKIYRLT RIYGRCE

References

[1]

"Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-alpha-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli." Goda S.K., Eissa O., Akhtar M., Minton N.P. Microbiology 143:3287-3294(1997) [PubMed: 9353929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 19398 / DSM 10702 / JCM 1391 / NCIMB 7423.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37874 Genomic DNA. Translation: AAB84229.1.
3D structure databases
ProteinModelPortal	Q59266.
ModBase	Search...
Protein family/group databases
CAZy	GH77. Glycoside Hydrolase Family 77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.4.1.25. 1465.
Family and domain databases
InterPro	IPR003385. Glyco_hydro_77. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02446. Glyco_hydro_77. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR00217. MalQ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MALQ_CLOBU

Accession

Primary (citable) accession number: Q59266

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program