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Q59263

- RIBF_CORAM

UniProt

Q59263 - RIBF_CORAM

Protein

Riboflavin biosynthesis protein RibF

Gene

ribF

Organism
Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.
    ATP + FMN = diphosphate + FAD.

    Enzyme regulationi

    Higher divalent cation concentrations lead to decrease in the turnover of riboflavin and the 5'-phosphotransferase activity, while the adenylyltransferase activity increases. With magnesium ions the highest turnover of riboflavin is observed between pH 6 and 7.5, while that of FAD is between 7.0 and 9.0. With zinc ions a steady increase in riboflavin turnover is observed between pH 4.5 and 10, while FAD is the major product at pH 7.0 and decreases rapidly above pH 8.0.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. FMN adenylyltransferase activity Source: UniProtKB-EC
    3. riboflavin kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. FAD biosynthetic process Source: UniProtKB-UniPathway
    2. FMN biosynthetic process Source: UniProtKB-UniPathway
    3. riboflavin biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.7.2. 1639.
    UniPathwayiUPA00276; UER00406.
    UPA00277; UER00407.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin biosynthesis protein RibF
    Including the following 2 domains:
    Riboflavin kinase (EC:2.7.1.26)
    Alternative name(s):
    Flavokinase
    FMN adenylyltransferase (EC:2.7.7.2)
    Alternative name(s):
    FAD pyrophosphorylase
    FAD synthase
    Gene namesi
    Name:ribF
    OrganismiCorynebacterium ammoniagenes (Brevibacterium ammoniagenes)
    Taxonomic identifieri1697 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338Riboflavin biosynthesis protein RibFPRO_0000194136Add
    BLAST

    Structurei

    Secondary structure

    1
    338
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi7 – 93
    Beta strandi17 – 226
    Helixi29 – 4517
    Beta strandi48 – 569
    Helixi58 – 625
    Beta strandi70 – 723
    Helixi74 – 8310
    Beta strandi87 – 926
    Turni94 – 963
    Beta strandi97 – 1004
    Helixi103 – 1097
    Helixi110 – 1145
    Beta strandi117 – 1237
    Beta strandi127 – 1293
    Helixi130 – 1323
    Helixi136 – 1427
    Turni143 – 1464
    Beta strandi147 – 1526
    Helixi164 – 1729
    Helixi176 – 1838
    Beta strandi188 – 1925
    Beta strandi200 – 2023
    Beta strandi208 – 2136
    Beta strandi222 – 23110
    Beta strandi239 – 2413
    Beta strandi247 – 25711
    Turni258 – 2603
    Beta strandi264 – 2718
    Beta strandi282 – 29211
    Helixi300 – 32324
    Turni325 – 3273
    Beta strandi330 – 3334

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2X0KX-ray1.95A/B1-338[»]
    3ZUGX-ray2.05A/B1-338[»]
    ProteinModelPortaliQ59263.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ59263.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RibF family.Curated

    Family and domain databases

    Gene3Di2.40.30.30. 1 hit.
    3.40.50.620. 1 hit.
    InterProiIPR015864. FAD_synthase.
    IPR023468. Riboflavin_kinase.
    IPR002606. Riboflavin_kinase_bac.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR22749. PTHR22749. 1 hit.
    PfamiPF06574. FAD_syn. 1 hit.
    PF01687. Flavokinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004491. FAD_Synth. 1 hit.
    SMARTiSM00904. Flavokinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF82114. SSF82114. 1 hit.
    TIGRFAMsiTIGR00083. ribF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q59263-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIWYGTAAV PKDLDNSAVT IGVFDGVHRG HQKLINATVE KAREVGAKAI    50
    MVTFDPHPVS VFLPRRAPLG ITTLAERFAL AESFGIDGVL VIDFTRELSG 100
    TSPEKYVEFL LEDTLHASHV VVGANFTFGE NAAGTADSLR QICQSRLTVD 150
    VIDLLDDEGV RISSTTVREF LSEGDVARAN WALGRHFYVT GPVVRGAGRG 200
    GKELGFPTAN QYFHDTVALP ADGVYAGWLT ILPTEAPVSG NMEPEVAYAA 250
    AISVGTNPTF GDEQRSVESF VLDRDADLYG HDVKVEFVDH VRAMEKFDSV 300
    EQLLEVMAKD VQKTRTLLAQ DVQAHKMAPE TYFLQAES 338
    Length:338
    Mass (Da):36,844
    Last modified:November 1, 1996 - v1
    Checksum:iB714FD885412CC68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37967 Genomic DNA. Translation: BAA07182.1.
    PIRiJC4008.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37967 Genomic DNA. Translation: BAA07182.1 .
    PIRi JC4008.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2X0K X-ray 1.95 A/B 1-338 [» ]
    3ZUG X-ray 2.05 A/B 1-338 [» ]
    ProteinModelPortali Q59263.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00276 ; UER00406 .
    UPA00277 ; UER00407 .
    BRENDAi 2.7.7.2. 1639.

    Miscellaneous databases

    EvolutionaryTracei Q59263.

    Family and domain databases

    Gene3Di 2.40.30.30. 1 hit.
    3.40.50.620. 1 hit.
    InterProi IPR015864. FAD_synthase.
    IPR023468. Riboflavin_kinase.
    IPR002606. Riboflavin_kinase_bac.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR22749. PTHR22749. 1 hit.
    Pfami PF06574. FAD_syn. 1 hit.
    PF01687. Flavokinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004491. FAD_Synth. 1 hit.
    SMARTi SM00904. Flavokinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82114. SSF82114. 1 hit.
    TIGRFAMsi TIGR00083. ribF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the FAD synthetase gene from Corynebacterium ammoniagenes and its expression in Escherichia coli."
      Nakagawa S., Igarashi A., Ohta T., Hagihara T., Fujio T., Aisaka K.
      Biosci. Biotechnol. Biochem. 59:694-702(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.
    2. "Purification and characterization of FAD synthetase from Brevibacterium ammoniagenes."
      Manstein D.J., Pai E.F.
      J. Biol. Chem. 261:16169-16173(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-18, CHARACTERIZATION.
      Strain: ATCC 6872 / DSM 20305 / KCTC 1019 / NCTC 2399.

    Entry informationi

    Entry nameiRIBF_CORAM
    AccessioniPrimary (citable) accession number: Q59263
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3