Reviewed, UniProtKB/Swiss-Prot Q59229 (AK2_BACSG)

Names and origin

Protein names

Recommended name:
    Aspartokinase 2
    EC=2.7.2.4
Alternative name(s):
    Aspartokinase II
    Aspartate kinase 2

Gene names

Name:

lysC

Organism

Bacillus sp. (strain MGA3)

Taxonomic identifier

126782 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Lysine-sensitive.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known).
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: Q59229-1)

Also known as: Aspartokinase 2 subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q59229-2)

Also known as: Aspartokinase 2 subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Aspartokinase 2		PRO_0000002369
Regions
	Domain	263 – 336	74	ACT 1
	Domain	343 – 408	66	ACT 2
Natural variations
	Alternative sequence	1 – 245	245	Missing in isoform Beta.		VSP_018653

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase 2 subunit alpha) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 94061572E369CD48	FASTA	411	44,343
	10 20 30 40 50 60 MGLIVQKFGG TSVGSVERIL NVANRVIEEK KNGNDVVVVV SAMGKTTDEL VDLAKQISAH 70 80 90 100 110 120 PPKREMDMLL TTGEQVTISL LAMALNEKGY EAISYTGWQA GITTEPVFGN ARILNIETEK 130 140 150 160 170 180 IQKQLNEGKI VVVAGFQGID EHGEITTLGR GGSDTTAVAL AAALKAEKCD IYTDVTGVFT 190 200 210 220 230 240 TDPRYVKSAR KLASISYDEM LELANLGAGV LHPRAVEFAK NYGITLEVRS SMEREEGTII 250 260 270 280 290 300 EEEVTMEQNL VVRGVAFEDE ITRVTVFGLP NSLTSLSTIF TTLAQNRINV DIIIQSATDA 310 320 330 340 350 360 ETTNLSFSIK SDDLEETMAV LENNKNLLNY QGIESETGLA KVSIVGSGMI SNPGVAAKMF 370 380 390 400 410 EVLALNGIQV KMVSTSEIKV STVVEESQMI KAVEALHQAF ELSGSAVKSE R
	Isoform Beta (Aspartokinase 2 subunit beta). Checksum: E93DD40FB43DBA8E	FASTA	166	17,898
	10 20 30 40 50 60 MEQNLVVRGV AFEDEITRVT VFGLPNSLTS LSTIFTTLAQ NRINVDIIIQ SATDAETTNL 70 80 90 100 110 120 SFSIKSDDLE ETMAVLENNK NLLNYQGIES ETGLAKVSIV GSGMISNPGV AAKMFEVLAL 130 140 150 160 NGIQVKMVST SEIKVSTVVE ESQMIKAVEA LHQAFELSGS AVKSER

References

[1]	"Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp." Schendel F.J., Flickinger M.C. Appl. Environ. Microbiol. 58:2806-2814(1992) [PubMed: 1444390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M93419 Genomic DNA. Translation: AAA22251.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK2_BACSG

Accession

Primary (citable) accession number: Q59229

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)