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Q59223

- THER_BACSO

UniProt

Q59223 - THER_BACSO

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Protein

Thermolysin

Gene

npr

Organism
Bacillus sp. (strain EA1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease. Has collagenase activity.

Catalytic activityi

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactori

Binds 4 calcium ions per subunit.
Binds 1 zinc ion per subunit.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi287 – 2871Calcium 1By similarity
Metal bindingi289 – 2891Calcium 1By similarity
Metal bindingi291 – 2911Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi368 – 3681Calcium 2By similarity
Metal bindingi372 – 3721Zinc; catalyticPROSITE-ProRule annotation
Active sitei373 – 3731PROSITE-ProRule annotation
Metal bindingi376 – 3761Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi396 – 3961Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi413 – 4131Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi415 – 4151Calcium 2By similarity
Metal bindingi415 – 4151Calcium 3By similarity
Metal bindingi417 – 4171Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi420 – 4201Calcium 2By similarity
Metal bindingi420 – 4201Calcium 3By similarity
Metal bindingi423 – 4231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi424 – 4241Calcium 4By similarity
Metal bindingi427 – 4271Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi430 – 4301Calcium 4By similarity
Active sitei461 – 4611Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolysin (EC:3.4.24.27)
Alternative name(s):
Thermostable neutral proteinase
Gene namesi
Name:npr
OrganismiBacillus sp. (strain EA1)
Taxonomic identifieri246599 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 228203Activation peptidePRO_0000028588Add
BLAST
Chaini229 – 546318ThermolysinPRO_0000028589Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ59223.
SMRiQ59223. Positions 228-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKRAMLGAI GLAFGLMAWP FGASAKEKSM VWNEQWKTPS FVSGSLLKGE
60 70 80 90 100
DAPEELVYRY LDQEKNTFQL GGQARERLSL IGKQTDELGH TVMRFEQRYR
110 120 130 140 150
GIPVYGAVLV AHVNDGELSS LSGTLIPNLD KRTLKTEAAI SIQQAEMIAK
160 170 180 190 200
QDVADAVTKE RPAAEEGKPT RLVIYPDGET PRLAYEVNVR FLTPVPGNWI
210 220 230 240 250
YMIDAADGKV LNKWNQMDEA KPGGGQPVAG TSTVGVGRGV LGDQKYINTT
260 270 280 290 300
YSSYYGYYYL QDNTRGSGIF TYDGRNRTVL PGSLWADVDN QFFASYDAAA
310 320 330 340 350
VDAHYYAGVV YDYYKNVHGR LSYDGSNAAI RSTVHYGRGY NNAFWNGSQM
360 370 380 390 400
VYGDGDGQTF LPFSGGIDVV GHELTHAVTD YTAGLVYQNE SGAINEAMSD
410 420 430 440 450
IFGTLVEFYA NRNPDWEIGE DIYTPGIAGD ALRSMSDPAK YGDPDHYSKR
460 470 480 490 500
YTGTQDNGGV HTNSGIINKA AYLLSQGGVH YGVSVTGIGR DKMGKIFYRA
510 520 530 540
LVYYLTPTSN FSQLRAACVQ AAADLYGSTS QEVNSVKQAF NAVGVY
Length:546
Mass (Da):59,813
Last modified:November 1, 1996 - v1
Checksum:i38019EDB8F32BFD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25630 Genomic DNA. Translation: AAB18653.1.
PIRiS72175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25630 Genomic DNA. Translation: AAB18653.1 .
PIRi S72175.

3D structure databases

ProteinModelPortali Q59223.
SMRi Q59223. Positions 228-545.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the gene encoding a highly thermostable neutral proteinase from Bacillus sp. strain EA1: expression in Escherichia coli and characterisation."
    Saul D.J., Williams L.C., Toogood H.S., Daniel R.M., Bergquist P.L.
    Biochim. Biophys. Acta 1308:74-80(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTHER_BACSO
AccessioniPrimary (citable) accession number: Q59223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3