Q59219 (IABF_BACOV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intracellular exo-alpha-L-arabinofuranosidase
Short name=ABF
EC=3.2.1.55

Alternative name(s):
Intracellular arabinan exo-alpha-L-arabinosidase
Short name=Arabinosidase

Gene names

Name:

asdII

Organism

Bacteroides ovatus

Taxonomic identifier

28116 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleveage of terminal alpha-L-arabinofuranosyl residues in different hemicellulosic homopolysaccharides (branched and debranched arabinans) and heteropolysaccharides (arabinoxylans) By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Pathway	Glycan metabolism; L-arabinan degradation.
Subunit structure	Homohexamer; trimer of dimers By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro arabinan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Intracellular exo-alpha-L-arabinofuranosidase		PRO_0000057703
Regions
	Region	194 – 195	2	Substrate binding By similarity
Sites
	Active site	195	1	Proton donor/acceptor By similarity
	Active site	317	1	Nucleophile By similarity
	Binding site	47	1	Substrate By similarity
	Binding site	93	1	Substrate; via amide nitrogen By similarity
	Binding site	261	1	Substrate By similarity
	Binding site	317	1	Substrate By similarity
	Binding site	366	1	Substrate By similarity
	Site	321	1	Important for substrate recognition By similarity
	Site	366	1	Important for substrate recognition By similarity
Amino acid modifications
	Disulfide bond	93 ↔ 199		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59219 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C8E1023A2E098884	FASTA	514	57,979
	10 20 30 40 50 60 MKAKLLVSTA FLAASVSLSA QKSATITVHA DQGKEIIPKE IYGQFAEHLG SCIYGGLWVG 70 80 90 100 110 120 ENSDIPNIKG YRTDVFNALK DLSVPVLRWP GGCFADEYHW MDGIGPKENR PKMVNNNWGG 130 140 150 160 170 180 TIEDNSFGTH EFLNLCEMLG CEPYVSGNVG SGTVEELAKW VEYMTSDGDS PMANLRRKNG 190 200 210 220 230 240 RDKAWKLKYL GVGNESWGCG GSMRPEYYAD LYRRYSTYCR NYDGNRLFKI ASGASDYDYK 250 260 270 280 290 300 WTDVLMNRVG HRMDGLSLHY YTVTGWSGSK GSATQFNKDD YYWTMGKCLE VEDVLKKHCT 310 320 330 340 350 360 IMDKYDKDKK IALLLDEWGT WWDEEPGTIK GHLYQQNTLR DAFVASLSLD VFHKYTDRLK 370 380 390 400 410 420 MANIAQIVNV LQSMILTKDK EMVLTPTYYV FKMYKVHQDA TYLPIDLTCE KMSVRDNRTV 430 440 450 460 470 480 PMVSATASKN KDGVIHISLS NVDADEAQEI TINLGDTKAK KAIGEILTAS KLTDYNSFEK 490 500 510 PNIVKPAPFK EVKINKGTMK VKLPAKSIVT LELQ

References

[1]	Whitehead T.R. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: V975.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15179 Genomic DNA. Translation: AAA50393.1.
3D structure databases
ProteinModelPortal	Q59219.
SMR	Q59219. Positions 23-514.
ModBase	Search...
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00667.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR010720. Alpha-L-AF_C. IPR013780. Glyco_hydro_13_b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IABF_BACOV

Accession

Primary (citable) accession number: Q59219

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program