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Q59219 (IABF_BACOV) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intracellular exo-alpha-L-arabinofuranosidase

Short name=ABF
EC=3.2.1.55
Alternative name(s):
Intracellular arabinan exo-alpha-L-arabinosidase
Short name=Arabinosidase
Gene names
Name:asdII
OrganismBacteroides ovatus
Taxonomic identifier28116 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-L-arabinofuranosyl residues in different hemicellulosic homopolysaccharides (branched and debranched arabinans) and heteropolysaccharides (arabinoxylans) By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathway

Glycan metabolism; L-arabinan degradation.

Subunit structure

Homohexamer; trimer of dimers By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Gene Ontology (GO)
   Biological_processL-arabinose metabolic process

Inferred from electronic annotation. Source: InterPro

arabinan catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-L-arabinofuranosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Intracellular exo-alpha-L-arabinofuranosidase
PRO_0000057703

Regions

Region194 – 1952Substrate binding By similarity

Sites

Active site1951Proton donor/acceptor By similarity
Active site3171Nucleophile By similarity
Binding site471Substrate By similarity
Binding site931Substrate; via amide nitrogen By similarity
Binding site2611Substrate By similarity
Binding site3171Substrate By similarity
Binding site3661Substrate By similarity
Site3211Important for substrate recognition By similarity
Site3661Important for substrate recognition By similarity

Amino acid modifications

Disulfide bond93 ↔ 199 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59219 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C8E1023A2E098884

FASTA51457,979
        10         20         30         40         50         60 
MKAKLLVSTA FLAASVSLSA QKSATITVHA DQGKEIIPKE IYGQFAEHLG SCIYGGLWVG 

        70         80         90        100        110        120 
ENSDIPNIKG YRTDVFNALK DLSVPVLRWP GGCFADEYHW MDGIGPKENR PKMVNNNWGG 

       130        140        150        160        170        180 
TIEDNSFGTH EFLNLCEMLG CEPYVSGNVG SGTVEELAKW VEYMTSDGDS PMANLRRKNG 

       190        200        210        220        230        240 
RDKAWKLKYL GVGNESWGCG GSMRPEYYAD LYRRYSTYCR NYDGNRLFKI ASGASDYDYK 

       250        260        270        280        290        300 
WTDVLMNRVG HRMDGLSLHY YTVTGWSGSK GSATQFNKDD YYWTMGKCLE VEDVLKKHCT 

       310        320        330        340        350        360 
IMDKYDKDKK IALLLDEWGT WWDEEPGTIK GHLYQQNTLR DAFVASLSLD VFHKYTDRLK 

       370        380        390        400        410        420 
MANIAQIVNV LQSMILTKDK EMVLTPTYYV FKMYKVHQDA TYLPIDLTCE KMSVRDNRTV 

       430        440        450        460        470        480 
PMVSATASKN KDGVIHISLS NVDADEAQEI TINLGDTKAK KAIGEILTAS KLTDYNSFEK 

       490        500        510 
PNIVKPAPFK EVKINKGTMK VKLPAKSIVT LELQ 

« Hide

References

[1]Whitehead T.R.
Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: V975.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15179 Genomic DNA. Translation: AAA50393.1.

3D structure databases

ProteinModelPortalQ59219.
SMRQ59219. Positions 23-514.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH51. Glycoside Hydrolase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00667.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR010720. Alpha-L-AF_C.
IPR013780. Glyco_hydro_13_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIABF_BACOV
AccessionPrimary (citable) accession number: Q59219
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries