Reviewed, UniProtKB/Swiss-Prot Q59214 (K6PF_BACMC)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk

Organism

Bacillus macquariensis (Paenibacillus macquariensis)

Taxonomic identifier

1468 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subunit structure	Homotetramer By similarity. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	6-phosphofructokinase HAMAP MF_00339		PRO_0000111933
Regions
	Nucleotide binding	23 – 27	5	ATP By similarity
	Nucleotide binding	156 – 160	5	ATP By similarity
	Nucleotide binding	173 – 189	17	ATP By similarity
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	187	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	189	1	Magnesium By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59214-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2C156F42669EC46C	FASTA	322	34,649
	10 20 30 40 50 60 MTIKKIAVLT SGGDSQGMNA AVRAVVRSGL FYGLEVYGIQ RGYQGLLNDD IFSMDLRSVG 70 80 90 100 110 120 DIIQRGGTVL QSARCKEFMT PEGQQKGADI LRKRGIDGLV VIGGDGSYHG ANKLSKLGIN 130 140 150 160 170 180 TMALPGTIDN DISYTDFTIG FDTSVSIVVD AINKLRDTMS SHERSSIVEV MGRHCGDIAL 190 200 210 220 230 240 YAGLASGAET IIVPEVPFDM DEIAERMKQN FAHGKRHSIV VVAEGAGNGE NVAKQLVERC 250 260 270 280 290 300 ETLEPRVTVL GHIQRGGTPT PADRNLASRL GDFAVRMLIA GESAKACGII SNELVLTDID 310 320 KVVNSKKEFN MELYELAARL SQ

References

[1]

"Cloning, sequence and expression in Escherichia coli of the gene encoding phosphofructokinase from Bacillus macquariensis." Rouwendal G.J.A., Zwiers L.H., Wolbert E.J.H., Springer J., Mooibroek H., Huizing H.J. Gene 171:59-63(1996) [PubMed: 8675031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23464 / DSM 2 / LMG 13289 / NCIB 9934 / NCTC 10419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76484 Genomic DNA. Translation: CAA54022.1.
PIR	S39248.
3D structure databases
SMR	Q59214. Positions 3-321.
ModBase	Search...
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_BACMC

Accession

Primary (citable) accession number: Q59214

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)