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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Paenibacillus macquariensis (Bacillus macquariensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi105Magnesium; catalyticUniRule annotation1
Active sitei129Proton acceptorUniRule annotation1
Binding sitei156Allosteric activator ADPUniRule annotation1
Binding sitei164Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei224SubstrateUniRule annotation1
Binding sitei246Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75ATPUniRule annotation2
Nucleotide bindingi104 – 107ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ59214
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfk
OrganismiPaenibacillus macquariensis (Bacillus macquariensis)
Taxonomic identifieri1468 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119331 – 322ATP-dependent 6-phosphofructokinaseAdd BLAST322

Proteomic databases

PRIDEiQ59214

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ59214
SMRiQ59214
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 27Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni127 – 129Substrate bindingUniRule annotation3
Regioni171 – 173Substrate bindingUniRule annotation3
Regioni187 – 189Allosteric activator ADP bindingUniRule annotation3
Regioni215 – 217Allosteric activator ADP bindingUniRule annotation3
Regioni252 – 255Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q59214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKKIAVLT SGGDSQGMNA AVRAVVRSGL FYGLEVYGIQ RGYQGLLNDD
60 70 80 90 100
IFSMDLRSVG DIIQRGGTVL QSARCKEFMT PEGQQKGADI LRKRGIDGLV
110 120 130 140 150
VIGGDGSYHG ANKLSKLGIN TMALPGTIDN DISYTDFTIG FDTSVSIVVD
160 170 180 190 200
AINKLRDTMS SHERSSIVEV MGRHCGDIAL YAGLASGAET IIVPEVPFDM
210 220 230 240 250
DEIAERMKQN FAHGKRHSIV VVAEGAGNGE NVAKQLVERC ETLEPRVTVL
260 270 280 290 300
GHIQRGGTPT PADRNLASRL GDFAVRMLIA GESAKACGII SNELVLTDID
310 320
KVVNSKKEFN MELYELAARL SQ
Length:322
Mass (Da):34,649
Last modified:November 1, 1996 - v1
Checksum:i2C156F42669EC46C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76484 Genomic DNA Translation: CAA54022.1
PIRiS39248
RefSeqiWP_068587249.1, NZ_LVJF01000025.1

Entry informationi

Entry nameiPFKA_PAEMC
AccessioniPrimary (citable) accession number: Q59214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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