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Q59214

- PFKA_PAEMC

UniProt

Q59214 - PFKA_PAEMC

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Paenibacillus macquariensis (Bacillus macquariensis)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
Active sitei129 – 1291Proton acceptorUniRule annotation
Binding sitei156 – 1561Allosteric activator ADPUniRule annotation
Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Binding sitei246 – 2461Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 752ATPUniRule annotation
Nucleotide bindingi104 – 1074ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ59214.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfk
OrganismiPaenibacillus macquariensis (Bacillus macquariensis)
Taxonomic identifieri1468 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322ATP-dependent 6-phosphofructokinasePRO_0000111933Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ59214.
SMRiQ59214. Positions 3-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 275Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni127 – 1293Substrate bindingUniRule annotation
Regioni171 – 1733Substrate bindingUniRule annotation
Regioni187 – 1893Allosteric activator ADP bindingUniRule annotation
Regioni215 – 2173Allosteric activator ADP bindingUniRule annotation
Regioni252 – 2554Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59214-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIKKIAVLT SGGDSQGMNA AVRAVVRSGL FYGLEVYGIQ RGYQGLLNDD
60 70 80 90 100
IFSMDLRSVG DIIQRGGTVL QSARCKEFMT PEGQQKGADI LRKRGIDGLV
110 120 130 140 150
VIGGDGSYHG ANKLSKLGIN TMALPGTIDN DISYTDFTIG FDTSVSIVVD
160 170 180 190 200
AINKLRDTMS SHERSSIVEV MGRHCGDIAL YAGLASGAET IIVPEVPFDM
210 220 230 240 250
DEIAERMKQN FAHGKRHSIV VVAEGAGNGE NVAKQLVERC ETLEPRVTVL
260 270 280 290 300
GHIQRGGTPT PADRNLASRL GDFAVRMLIA GESAKACGII SNELVLTDID
310 320
KVVNSKKEFN MELYELAARL SQ
Length:322
Mass (Da):34,649
Last modified:November 1, 1996 - v1
Checksum:i2C156F42669EC46C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76484 Genomic DNA. Translation: CAA54022.1.
PIRiS39248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76484 Genomic DNA. Translation: CAA54022.1 .
PIRi S39248.

3D structure databases

ProteinModelPortali Q59214.
SMRi Q59214. Positions 3-321.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK Q59214.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequence and expression in Escherichia coli of the gene encoding phosphofructokinase from Bacillus macquariensis."
    Rouwendal G.J.A., Zwiers L.H., Wolbert E.J.H., Springer J., Mooibroek H., Huizing H.J.
    Gene 171:59-63(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23464 / DSM 2 / LMG 13289 / NCIMB 9934 / NCTC 10419 / NRRL B-14306.

Entry informationi

Entry nameiPFKA_PAEMC
AccessioniPrimary (citable) accession number: Q59214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3