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Q59214

- PFKA_PAEMC

UniProt

Q59214 - PFKA_PAEMC

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, pfk
Organism
Paenibacillus macquariensis (Bacillus macquariensis)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogen By similarity
Metal bindingi105 – 1051Magnesium; catalytic By similarity
Active sitei129 – 1291Proton acceptor By similarity
Binding sitei156 – 1561Allosteric activator ADP By similarity
Binding sitei164 – 1641Substrate; shared with dimeric partner By similarity
Binding sitei224 – 2241Substrate By similarity
Binding sitei246 – 2461Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 752ATP By similarity
Nucleotide bindingi104 – 1074ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ59214.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
OrganismiPaenibacillus macquariensis (Bacillus macquariensis)
Taxonomic identifieri1468 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000111933Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ59214.
SMRiQ59214. Positions 3-321.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 275Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni127 – 1293Substrate binding By similarity
Regioni171 – 1733Substrate binding By similarity
Regioni187 – 1893Allosteric activator ADP binding By similarity
Regioni215 – 2173Allosteric activator ADP binding By similarity
Regioni252 – 2554Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59214-1 [UniParc]FASTAAdd to Basket

« Hide

MTIKKIAVLT SGGDSQGMNA AVRAVVRSGL FYGLEVYGIQ RGYQGLLNDD    50
IFSMDLRSVG DIIQRGGTVL QSARCKEFMT PEGQQKGADI LRKRGIDGLV 100
VIGGDGSYHG ANKLSKLGIN TMALPGTIDN DISYTDFTIG FDTSVSIVVD 150
AINKLRDTMS SHERSSIVEV MGRHCGDIAL YAGLASGAET IIVPEVPFDM 200
DEIAERMKQN FAHGKRHSIV VVAEGAGNGE NVAKQLVERC ETLEPRVTVL 250
GHIQRGGTPT PADRNLASRL GDFAVRMLIA GESAKACGII SNELVLTDID 300
KVVNSKKEFN MELYELAARL SQ 322
Length:322
Mass (Da):34,649
Last modified:November 1, 1996 - v1
Checksum:i2C156F42669EC46C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76484 Genomic DNA. Translation: CAA54022.1.
PIRiS39248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76484 Genomic DNA. Translation: CAA54022.1 .
PIRi S39248.

3D structure databases

ProteinModelPortali Q59214.
SMRi Q59214. Positions 3-321.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK Q59214.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequence and expression in Escherichia coli of the gene encoding phosphofructokinase from Bacillus macquariensis."
    Rouwendal G.J.A., Zwiers L.H., Wolbert E.J.H., Springer J., Mooibroek H., Huizing H.J.
    Gene 171:59-63(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23464 / DSM 2 / LMG 13289 / NCIMB 9934 / NCTC 10419 / NRRL B-14306.

Entry informationi

Entry nameiPFKA_PAEMC
AccessioniPrimary (citable) accession number: Q59214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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