Reviewed, UniProtKB/Swiss-Prot Q59212 (TYSY_BACLI)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45
Alternative name(s):
    Thymidylate synthase A
      Short name=TS A
      Short name=TSase A

Gene names

Name:	thyA
Synonyms:	thyBL

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Thymidylate synthase HAMAP MF_00008		PRO_0000140927
Sites
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59212-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 479D3E24E452EA8F	FASTA	279	32,760
	10 20 30 40 50 60 MSHYDQQYNA IIQKIIESGI SDEEYQVRTK WDSDGTPAHT LSIMSEKMRF DNSEVPILTT 70 80 90 100 110 120 KKVAWKTAIK ELLWIWQLKS NDVQVLNDMG VHIWDQWRLE DGTIGAAYGY QLGKKNRTVN 130 140 150 160 170 180 GQKVDQVDYL LHQLKHNPSS RRHLTMLWNP DDLDGMALTP CVYETQWYVK EGKLSLEVRA 190 200 210 220 230 240 RSNDMALGNP FNVFQYNVLQ RMIAQVLGYE LGEYIFNIGD CHIYTRHIDN LNIQMKREQY 250 260 270 EAPKLWINPD IKNFYDFTID DFKLIDYKHG DKLTFEVAV

References

[1]	"Cloning of the Bacillus thymidylate synthase genes thyBA and thyBL." Steinborn G., Richter K. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9789 / DSM 8785 / IMET 11025 / NCTC 6346.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50165 Genomic DNA. Translation: CAA90533.1.
3D structure databases
HSSP	HSSP built from PDB template 1BSP based on UniProtKB P42326.
SMR	Q59212. Positions 2-279.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.45. 1017.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACLI

Accession

Primary (citable) accession number: Q59212

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)