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Q59207 (FIXI_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitrogen fixation protein FixI
Alternative name(s):
E1-E2 type cation ATPase FixI
EC=3.6.3.-
Gene names
Name:fixI
Ordered Locus Names:blr2769
OrganismBradyrhizobium japonicum
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length730 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification]

Contains 1 HMA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 730730Nitrogen fixation protein FixI
PRO_0000046154

Regions

Topological domain1 – 101101Cytoplasmic Potential
Transmembrane102 – 12322Helical; Potential
Topological domain124 – 13815Extracellular Potential
Transmembrane139 – 16224Helical; Potential
Topological domain163 – 1686Cytoplasmic Potential
Transmembrane169 – 19022Helical; Potential
Topological domain191 – 20212Extracellular Potential
Transmembrane203 – 22321Helical; Potential
Topological domain224 – 352129Cytoplasmic Potential
Transmembrane353 – 37523Helical; Potential
Topological domain376 – 3827Extracellular Potential
Transmembrane383 – 40018Helical; Potential
Topological domain401 – 676276Cytoplasmic Potential
Transmembrane677 – 69620Helical; Potential
Topological domain697 – 7015Extracellular Potential
Transmembrane702 – 72019Helical; Potential
Topological domain721 – 73010Cytoplasmic Potential
Domain20 – 8667HMA

Sites

Active site43814-aspartylphosphate intermediate Probable
Metal binding301 Potential
Metal binding331 Potential
Metal binding6221Magnesium By similarity
Metal binding6261Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59207 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 41588689FE78AD25

FASTA73077,339
        10         20         30         40         50         60 
MHVTRDFSHY VRTAGEGIKH IDLAVEGVHC AGCMAKIERG LSAIPDVTLA RVNLTDRRVA 

        70         80         90        100        110        120 
LEWKAGTLDP GRFIDRLEEL GYKAYPFETE SAEVAEVAES RFLLRCLGVA AFATMNVMML 

       130        140        150        160        170        180 
SIPVWSGNVS DMLPEQRDFF HWLSALIALP AAAYAGQPFF RSAWRALSAK TTNMDVPISI 

       190        200        210        220        230        240 
GVILALGMSV VETIHHAEHA YFDAAIMLLT FLLVGRFLDQ NMRRRTRAVA GNLAALKAET 

       250        260        270        280        290        300 
AAKFVGPDEI SQVPVAAISP GDIVLLRPGE RCAVDGTVIE GRSEIDQSLI TGETLYVTAE 

       310        320        330        340        350        360 
QGTPVYAGSM NISGTLRVRV SAASEATLLA EIARLLDNAL QARSRYMRLA DRASRLYAPV 

       370        380        390        400        410        420 
VHATALITIL GWVIAGASWH DAIVTGVAVL IITCPCALGL AIPTVQTVAS GAMFKSGVLL 

       430        440        450        460        470        480 
NSGDAIERLA EADHVIFDKT GTLTLPDLEV MNAADIPADI FELAGRLALS SHHPVAAAVA 

       490        500        510        520        530        540 
QAAGARSPIV GAVEEAGQGV RADVDGAEIR LGRPSFCGAE ALVGDGTRLD PEASIVAFSK 

       550        560        570        580        590        600 
GAEKFILWVR QGLRPDAQAV IAALKARNIG IEILSGDREP AVKAAAHALA IPEWRAGVTP 

       610        620        630        640        650        660 
ADKIARIEEL KRRGARVLMV GDGMNDAPSL AAAHVSMSPI SAAHLSQATA DLVFLGRPLA 

       670        680        690        700        710        720 
PVAAAIDSAR KALHLMRQNL WLAIGYNVLA VPVAISGVVT PLIAAAAMSG SSILVMLNSL 

       730 
RARSDSREIV

« Hide

References

« Hide 'large scale' references
[1]"The Bradyrhizobium japonicum fixGHIS genes are required for the formation of the high-affinity cbb3-type cytochrome oxidase."
Preisig O., Zufferey R., Hennecke H.
Arch. Microbiol. 165:297-305(1996) [PubMed: 8661920] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X95634 Genomic DNA. Translation: CAA64889.1.
AJ005001 Genomic DNA. Translation: CAA06285.1.
BA000040 Genomic DNA. Translation: BAC48034.1.
RefSeqNP_769409.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ59207.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1054097.
GenomeReviewsGene locus blr2769 in contig BA000040_GR.
KEGGbja:blr2769.
NMPDRfig|224911.1.peg.2769.
PATRIC21188986. VBIBraJap65052_2751.

Phylogenomic databases

HOGENOMHBG507745.
OMADYSRKAM.
PhylomeDBQ59207.
ProtClustDBCLSK910881.

Enzyme and pathway databases

BioCycBJAP224911:BLR2769-MONOMER.

Family and domain databases

InterProIPR023306. ATPase_cation_domN.
IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR006403. ATPase_P-typ_cat/Cu-transptr.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006416. ATPase_P-typ_heavy-metal.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KOK01533.
PfamPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SUPFAMSSF81660. ATPase_cation_domN. 1 hit.
SSF56784. HAD-like_dom. 1 hit.
SSF55008. HeavyMe_transpt. 1 hit.
TIGRFAMsTIGR01511. ATPase-IB1_Cu. 1 hit.
TIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIXI_BRAJA
AccessionPrimary (citable) accession number: Q59207
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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