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Q59202

- MDH_BACIS

UniProt

Q59202 - MDH_BACIS

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Protein
Malate dehydrogenase
Gene
mdh
Organism
Bacillus israeli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361NAD By similarity
Binding sitei87 – 871Substrate By similarity
Binding sitei93 – 931Substrate By similarity
Binding sitei100 – 1001NAD By similarity
Binding sitei125 – 1251Substrate By similarity
Binding sitei156 – 1561Substrate By similarity
Active sitei180 – 1801Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NAD By similarity
Nucleotide bindingi123 – 1253NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
OrganismiBacillus israeli
Taxonomic identifieri42189 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Malate dehydrogenaseUniRule annotation
PRO_0000113431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei149 – 1491Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliQ59202.
SMRiQ59202. Positions 5-311.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59202-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKRKKISV IGAGFTGATT AFLLAKKELG DVVLVDIPQA ENPTKGKALD    50
MLESSPVLGF DANIIGTSNY EETADSDIVV ITAGIARKPG MSRDDLVQTN 100
QKVMKSVTKE VVKYSPNSII IVLTNPVDAM TYTVYKESGF PKHRVIGQSG 150
VLDTARFRTF VAQELNLSVK DITGFVLGGH GDDMVPLVRY SYAGGIPLEK 200
LIPKERLEAI VERTRKGGGE IVNLLGNGSA YYAPAASLVE MVEAIVKDQR 250
RVLPAIAYLE GEYGFEGIYL GVPTILGGNG LEQIIELELT DEEKAALEKS 300
AESVRNVMKA LI 312
Length:312
Mass (Da):33,648
Last modified:November 1, 1997 - v1
Checksum:i64CE3BFF1B3C02D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90527 Genomic DNA. Translation: CAA62129.1.
PIRiS61213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90527 Genomic DNA. Translation: CAA62129.1 .
PIRi S61213.

3D structure databases

ProteinModelPortali Q59202.
SMRi Q59202. Positions 5-311.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_00487. Malate_dehydrog_3.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
PRINTSi PR00086. LLDHDRGNASE.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Tetrameric malate dehydrogenase from a thermophilic Bacillus: cloning, sequence and overexpression of the gene encoding the enzyme and isolation and characterization of the recombinant enzyme."
    Wynne S.A., Nicholls D.J., Scawen M.D., Sundaram T.K.
    Biochem. J. 317:235-245(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMDH_BACIS
AccessioniPrimary (citable) accession number: Q59202
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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