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Q59193

- THER_BACCL

UniProt

Q59193 - THER_BACCL

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Protein

Thermolysin

Gene
npr
Organism
Bacillus caldolyticus
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactori

Binds 4 calcium ions per subunit.
Binds 1 zinc ion per subunit.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi287 – 2871Calcium 1 By similarity
Metal bindingi289 – 2891Calcium 1 By similarity
Metal bindingi291 – 2911Calcium 1; via carbonyl oxygen By similarity
Metal bindingi368 – 3681Calcium 2 By similarity
Metal bindingi372 – 3721Zinc; catalytic By similarity
Active sitei373 – 3731 By similarity
Metal bindingi376 – 3761Zinc; catalytic By similarity
Metal bindingi396 – 3961Zinc; catalytic By similarity
Metal bindingi413 – 4131Calcium 3; via carbonyl oxygen By similarity
Metal bindingi415 – 4151Calcium 2 By similarity
Metal bindingi415 – 4151Calcium 3 By similarity
Metal bindingi417 – 4171Calcium 2; via carbonyl oxygen By similarity
Metal bindingi420 – 4201Calcium 2 By similarity
Metal bindingi420 – 4201Calcium 3 By similarity
Metal bindingi423 – 4231Calcium 4; via carbonyl oxygen By similarity
Metal bindingi424 – 4241Calcium 4 By similarity
Metal bindingi427 – 4271Calcium 4; via carbonyl oxygen By similarity
Metal bindingi430 – 4301Calcium 4 By similarity
Active sitei461 – 4611Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolysin (EC:3.4.24.27)
Alternative name(s):
Thermostable neutral proteinase
Gene namesi
Name:npr
OrganismiBacillus caldolyticus
Taxonomic identifieri1394 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Propeptidei26 – 228203Activation peptidePRO_0000028586Add
BLAST
Chaini229 – 546318ThermolysinPRO_0000028587Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ59193.
SMRiQ59193. Positions 228-545.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59193-1 [UniParc]FASTAAdd to Basket

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MDKRAMLGAI GLAFGLMAWP FGASAKEKSM VWNEQWKTPS FVSGSLLKGE    50
DAPEELVYRY LDQEKNTFQL GGQARERLSL IGKQTDELGH TVMRFEQRYR 100
GIPVYGAVLV AHVNDGELSS LSGTLIPNLD KRTLKTEAAI SIQQAEMIAK 150
QDVADAVTKE RPAAEEGKPT RLVIYPDGET PRLAYEVNVR FLTPVPGNWI 200
YMIDAADGKV LNKWNQMDEA KPGGGQPVAG TSTVGVGRGV LGDQKYINTT 250
YSSYYGYYYL QDNTRGSGIF TYDGRNRTVL PGSLWADGDN QFFASYDAAA 300
VDAHYYAGVV YDYYKNVHGR LSYDGSNAAI RSTVHYGRGY NNAFWNGSQM 350
VYGDGDGQTF LPFSGGIDVV GHELTHAVTD YTAGLVYQNE SGAINEAMSD 400
IFGTLVEFYA NRNPDWEIGE DIYTPGIAGD ALRSMSDPAK YGDPDHYSKR 450
YTGTQDNGGV HTNSGIINKA AYLLSQGGVH YGVSVTGIGR DKMGKIFYRA 500
LVYYLTPTSN FSQLRAACVQ AAADLYGSTS QEVNSVKQAF NAVGVY 546
Length:546
Mass (Da):59,771
Last modified:November 1, 1996 - v1
Checksum:i38019807FF32B071
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25629 Genomic DNA. Translation: AAB18652.1.
PIRiS72176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25629 Genomic DNA. Translation: AAB18652.1 .
PIRi S72176.

3D structure databases

ProteinModelPortali Q59193.
SMRi Q59193. Positions 228-545.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the gene encoding a highly thermostable neutral proteinase from Bacillus sp. strain EA1: expression in Escherichia coli and characterisation."
    Saul D.J., Williams L.C., Toogood H.S., Daniel R.M., Bergquist P.L.
    Biochim. Biophys. Acta 1308:74-80(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 405 / NBRC 15313 / YP-T.

Entry informationi

Entry nameiTHER_BACCL
AccessioniPrimary (citable) accession number: Q59193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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