Reviewed, UniProtKB/Swiss-Prot Q59185 (LON1_BORBU)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	BB_0253

Organism

Borrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	806 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 806	806	ATP-dependent protease La		PRO_0000076118
Regions
	Domain	30 – 233	204	Lon
	Nucleotide binding	389 – 396	8	ATP Potential
Sites
	Active site	714	1	By similarity
	Active site	757	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59185-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 71F488D719B1424E	FASTA	806	90,710
	10 20 30 40 50 60 MDESKKARSG DKKKEKAVAG ILPHSNKPAR VPLIAVPSHP VFPGMFIPIV LISDSDMKAI 70 80 90 100 110 120 DYAMKGNGII ALFVLNDKFL EKNNNNAQQK LIIDYSKDIY SVGVTGKIIK KINLPDGGYN 130 140 150 160 170 180 IFVSTFDRIK FVKVVLNDKF PIIEIDYLKQ IPVRKDDIQS KAVYGSILLR TKEIFAHRKM 190 200 210 220 230 240 PEVQLNMVNI EDKGKLCDIV ASTISSSKND HQIVLETLNV KDRLKKVLEL IYEELNLIEI 250 260 270 280 290 300 QNKIAKGIQE RLEKQQKEFF LKEQLKAIKA ELGIGDKKSS DLEKLKTKLK ALELKGEPLE 310 320 330 340 350 360 VVEKELEKFS LLETSSAEYI VVRNYLELIT ELPWRDFKIN FDKLDLQKSK KILDKTHYGM 370 380 390 400 410 420 NEVKDRIIEY ISVLKLRKTQ KGAIILLVGP PGVGKTSIGA AIAKVLRTKF FRFSVGGMRD 430 440 450 460 470 480 ESEIKGHRRT YVGALPGKII QGLRITKTNS PVFLIDEVDK ISASSYGDPF SVLLEVLDPE 490 500 510 520 530 540 QNVRFRDHYL DLPFDISNVF FILTANSVET IPRPLLNRME IIEISGYIDN EKIEIARKYL 550 560 570 580 590 600 IPKVLSENGV DKDSLKFQSS SLVQIAQEYA RDNGVRNFEK YLNKIVRKVA RKLIENTEVK 610 620 630 640 650 660 SYQISNDNLE EYVGVPVFRK ESMPNAMYSG MVMGLAWTNY GGSTLMIETV KTESKVGGIK 670 680 690 700 710 720 LTGRLGDVMK ESANIAYTYV NSIKGDLSIS KSFFEKNIIH LHIPEGATPK DGPSAGITIA 730 740 750 760 770 780 SAFISLALNK VVRPHLAMTG ELSLTGNVMM IGGLKEKIIA AKRSGVEHII VPKANRVDLE 790 800 EIPTNIKSGI NFYLVDNMRE VIKLLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the Borrelia burgdorferi lon gene." Cloud J.L., Marconi R.T., Eggers C.H., Garon C.F., Tilly K., Samuels D.S. Gene 194:137-141(1997) [PubMed: 9266683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77216 Genomic DNA. Translation: AAB72011.1. AE000783 Genomic DNA. Translation: AAB91493.1.
PIR	E70131.
RefSeq	NP_212387.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195090.
GenomeReviews	Gene locus BB_0253 in contig AE000783_GR.
KEGG	bbu:BB0253.
NMPDR	fig|224326.1.peg.637.
TIGR	BB_0253.
Phylogenomic databases
HOGENOM	Q59185.
OMA	Q59185. VRNYLEL.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0253-MON.
BRENDA	3.4.21.53. 142596.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON1_BORBU

Accession

Primary (citable) accession number: Q59185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)